上海耐冷转基因材料与黑河大豆骨干亲本杂交组合配制技术

为了能够有效利用耐冷材料,2010年黑龙江农业科学院黑河分院引进上海耐冷大豆材料SZ1,通过本地种植鉴定,该材料在黑河地区无法正常成熟,生育期长,采用温室育苗与错期播种技术,经过2a时间摸索出一套外引晚熟大豆材料与黑河骨干亲本杂交组合配制技术。     

Effects of artesunate on membrane surface morphology of human gastric cancer SGC-7901 cells observed under atomic force microscope

AIM: To detect the membrane surface morphology of cancer cells treated with artesunate (ART) under atomic force microscope (AFM). METHODS: Human gastric cancer SGC-7901 cells were cultured and treated with different concentrations of ART for 24 h. The membrane surface morphology, three-dimensional structures and ultrastructural changes of SGC-7901 cells were observed under AFM. The apoptosis of SGC-7901 cells was detected by flow cytometry. RESULTS: The AFM images revealed that the cell nuclear area was full and the surface of cell membrane was flat and smooth in control group. Compared with control group, the cell nuclear area collapsed and suffered atrophy, and the membrane surface had more pores in ART treatment groups. More pores were observed and the diameter of the pores was increased with the increase in ART concentration. The cell membrane ultrastructure showed that the particles in control group had an intensive distribution, some were cord-shaped and some gathered into a mass. The particles in ART treatment groups were fewer and distributed sparsely. Moreover, there were obvious lacunae in the surface of cell membrane. Quantitive measurement found that the height of cell nucleus area was decreased, and the surface root mean square roughness (Rq) and the surface average roughness (Ra) became smaller in ART treatment groups compared with control group. The apoptotic rates of the cells in ART treatment groups were increased with the increase in ART concentration, and were all significantly higher than that in control group. CONCLUSION: Our findings emphasize the significance of AFM in exploring the changes of the membrane surface morphology at nano-scale resolution following the treatment with anticancer drugs, which could not only identify the specific characteristics of morphological changes of the tumor cells, but also provide micromorphological references to reveal the mechanisms of anticancer drugs.     

不同浓度氮酮 薄荷醇对酮康唑体外经皮渗透作用的影响

酮康唑(ketoconazole,KET)是一种常用的广谱抗真菌药物,对浅表性真菌感染及各种急、慢性真菌病口服均有效,但长期服用可引起肝脏损害等毒副作用,在使用上一直存在局限性.     

菊花发酵乳饮料工艺优化

以菊花提取液和鲜牛奶为主要原料，杀菌后接种乳酸菌进行乳酸发酵，通过单因素与正交试验确定原料的配比、发酵条件及产品的配方。结果表明，原料最佳配比为菊花提取汁20％、蔗糖8％、鲜牛奶40％；制备该乳饮料的最佳发酵工艺条件为接种量3％、发酵时间4h、温度42℃。     

西藏高校大学生考证现状研究

随着社会主义市场经济体制的深化,市场就业竞争压力与日俱增,西藏高校大学生纷纷加入"考证"的行列.本研究针对西藏高校大学生考证现状,以西藏大学作为典例进行分析,对大一至大四的本科生发放问卷及访谈,在成因分析的基础上提出引导西藏高校大学生正确考证的建议.     

早熟金柑新品种‘金秋早’

早熟金柑新品种‘金秋早’是从福建省尤溪县‘尤溪金柑’园中优良实生单株选育而成。生长健壮。果实近球形,果皮橙黄至橙红色,单果质量17~26 g,皮肉脆嫩化渣,酸甜可口,风味浓。果汁可溶性固形物含量13%,总酸0.8%,总糖9%,维生素C 394 mg·L~(-1)。每果种子数5~7个。成熟期在11月上旬,比‘尤溪金柑’早15~20 d。适宜在福建省尤溪金柑适栽区种植。     

硬盘数据的安全防御

随着信息和网络技术的快速发展,越来越多的重要数据存储在硬盘上,硬盘数据的安全保护逐渐成为备受关注的问题。通过对计算机硬盘数据结构的分析,结合人们对计算机硬盘数据安全的认识误区,全面阐述了威胁计算机硬盘数据安全的几个因素及其防范措施。     

血红素加氧酶1基因表达调控序列的克隆及应用研究

为了获得京尼平苷调节HO-1基因表达的直接证据,克隆了ho-1基因的表达调控序列,并将其构建到以荧光素酶为报告基因的p-GL-3质粒上,通过转染HEK293细胞,对京尼平苷调控报告基因的表达进行了分析,结果证实:京尼平苷能够剂量依赖的提高报告基因的表达水平,提示京尼平苷诱导HO-1水平上调与增强其转录有关.     

外源钙水平与花生下针期不同土壤水分对植株生理特性的影响

沿海耕作风砂土土壤缺钙与干旱常相伴发生。通过盆栽试验，研究了不同钙肥水平下花生下针期至结荚初期土壤水分管理对植株生理特性及产量的影响。结果表明，下针期(553)%（LW）、 (753)%（MW）和(953)%（HW）三种田间持水量条件下，叶片SPAD值、 净光合速率、 水分利用效率及产量均随着钙肥用量的增加而提高，其中施钙肥的花生荚果产量增幅12.2% ~19.4%，而三种钙肥水平下，MW比LW与HW的荚果产量分别显著提高18.7%与56.5%，说明下针期过高或过低的水分均不利于光合作用及产量形成，尤其高水量条件下，其根系活力显著下降。花生成熟期产量与下针期叶片SPAD值、 净光合速率、 水分利用效率及根系活力呈显著正相关; 不同水分管理与钙肥水平均显著影响花生产量，但水钙间产量交互效应不明显。耕作风砂土上增施钙肥增产潜力大，且一定程度上可缓解旱情，花生下针期调控约75%的田间持水量，其光合速率与产量最高。     

去年我国木制家具进出口状况大体与2008年持平

受世界性金融危机的影响,2008年我国木制家具出口数量出现了较大幅度的下降。这种状况在2009年却有了令人可喜的扭转,从国家海关最近发布的数据（参看表1—3）和比较中,我们可以清晰地发现,去年我国木制家具贸易无论是在出口或是在进口方面,也不管是进出口数量、价值金额，以及单价水平上，基本上稳住了局势，没有出现大幅度的跌荡，总体状况与2008年持平，此种形势在当前全球经济运行仍未走出萎靡不振的泥淖的现实中，是非常难得的。