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51.
Hideki YAMAMOTO Tadahiko KIGUCHI Mineo SENDA Kenji UEDA Masayasu INOUE Tsutomu MATSUMOTO 《Journal of General Plant Pathology》2001,67(4):309-311
The three missense mutations on the gene for the 130-K protein of Tomato mosaic virus (ToMV) L11A have been thought to be responsible for the attenuation of its virulence. The Eco47I RFLP detecting the missense mutation at 2349 successfully discriminated L11A and its derivative attenuated isolates from ToMV virulent ones. RFLP analysis and mismatch amplification assay detecting
the missense mutations at 1117 and 2754, respectively, could not discriminate some of the attenuated isolates from the virulent
ones. These results indicated that, of the three missense mutations, only the one at 2349 was conserved in all the L11A-derivative attenuated isolates.
Received 16 March 2001/ Accepted in revised form 22 June 2001 相似文献
52.
cDNA microarray analysis of gene expression in rice plants treated with probenazole,a chemical inducer of disease resistance 总被引:2,自引:0,他引:2
53.
54.
Hiroshi KAMIUNTEN Satoru INOUE Yasuko YAKABE Shigeru IIDA 《Journal of General Plant Pathology》2002,68(1):75-80
Two new active insertion sequences, ISPsy2 and ISPsy3, were isolated from Pseudomonas syringae pv. eriobotryae, the causal agent of stem cankers of loquat trees. ISPsy2 is 1194-bp long, has 16-bp imperfect terminal inverted repeats, and
generates a 4-bp target site duplication upon insertion into the selective cartridge of the entrap vector pSHI1063. The nucleotide
sequence of ISPsy2 is completely identical with that of the previously identified IS-like element located adjacent to the
virulence gene psvA of Pseudomonas syringae pv. eriobotryae NAE6. The single open reading frame of ISPsy2 encodes a 323-amino-acid protein that has similarity to the transposase of the
IS5 subgroup of the IS5 family. The ISPsy3 belonging to the IS91 family is 1507 bp in length, does not duplicate its target
sequence, GAAC, and presents an 81% sequence homology with IS801 in P. s. pv. phaseolicola. The transposase of ISPsy3
possesses the conserved amino acid motifs found in the rolling-circle replication protein. Southern blot analysis indicated
that multiple copies of ISPsy2 and ISPsy3 are present in the genomes of P. s. pv. eriobotryae and some of the other P. s. pathovars tested.
Received 16 August 2001/ Accepted in revised form 19 October 2001 相似文献
55.
The aim of the present study was to determine whether bovine coronavirus (BCV) has the ability to initiate infection in a human colon carcinoma cell line, Caco‐2, that has been established to spontaneously differentiate after confluence. When Caco‐2 cells were infected with BCV, a titer of 5.5 × 106 plaque‐forming units (p.f.u.)/mL was found in the culture supernatant at 5 days postinfection. Two clones, Caco‐2/CA1 and Caco‐2/CA2, were then isolated by monitoring alkaline phosphatase (ALP) and cell proliferation activities. The ALP activity level of CA1 cells was significantly higher than that of CA2 cells, while the level of cell proliferation activity of CA1 was significantly lower than that of CA2. When CA1 and CA2 cells were infected with BCV at confluence, virus hemagglutination (HA) was detected in the culture supernatant at 5 days postinfection for CA1 cells and at 8 days postinfection for CA2 cells. Thus, BCV propagation was substantially delayed in CA2 cells, suggesting that a cellular factor(s) that appears at the differentiation stage may control BCV propagation. BCV‐susceptible CA1 and CA2 cells showing different levels of ALP activity would be useful for further experiments to elucidate the mechanism of BCV propagation. 相似文献
56.
To clarify the establishment pattern of Cryptomeria japonica seedlings, the demography of current-year seedlings was studied under various micro-environmental conditions for 2 years in a cool-temperate old-growth forest in the snowy region of Japan. In both years, more than 70% of seedlings that emerged died within a year, and differences in the survival rate of seedlings with respect to micro-environmental conditions were similar. While a number of seedlings survived at the sites on fallen logs and in mineral soil, almost no seedlings survived at the sites with either broad-leaved or coniferous litters at the end of the first growing season. The negative litter effect may kill most small seedlings of C. japonica. In addition, we found that survival rates of the seedlings were greater as the distance from the nearest adult and canopy openness increased. An analysis using Quantification Theory I showed that, of the possible micro-environmental factors, substrate conditions had the strongest effect on the survival of seedlings. The analysis also indicated that distance from the nearest adult and canopy openness also affected seedling survival significantly and to almost the same extent, although the effects were weaker than those for the substrate condition. Given the limited establishment sites for C. japonica seedlings, layering is suggested to be the main mode of regeneration in this forest. 相似文献
57.
Saki INOUE Kayoko YONEMARU Tokuma YANAI Hiroki SAKAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):225-228
A 5-year-old male ferret presented with an enlarged canalicular testis in the left inguinal region. Microscopically, the enlarged testis consisted of a diffuse intimately admixed proliferation of c-kit-positive germ cell-like and Wilms tumor-1 protein-positive Sertoli cell-like components, but no Call-Exner body was detected. In addition, the compact proliferation of steroidogenic acute regulatory protein-intense positive interstitial cells was identified in a separate peripheral area of the mass. Based on histopathological and immunohistochemical findings, the tumor was diagnosed as a mixed germ cell-sex cord-stromal tumor with a concurrent interstitial cell tumor. 相似文献
58.
Thu-Thuy NGUYEN Mono Sophie MOTSIRI Moeti Oriel TAIOE Moses Sibusiso MTSHALI Yasuyuki GOTO Shin-Ichiro KAWAZU Oriel Matlhahane Molifi THEKISOE Noboru INOUE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):217-220
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6–0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in
on-site diagnosis, but its sensitivity should be improved. 相似文献
59.
Miki H Lee J Inoue K Ogonuki N Noguchi Y Mochida K Kohda T Nagashima H Ishino F Ogura A 《The Journal of reproduction and development》2004,50(1):131-137
In several mammalian species, including mice, round spermatids have been used to produce normal offspring by means of microinsemination techniques. In this study, we examined whether mouse round spermatids retrieved from immature testes undergoing the first wave of spermatogenesis had acquired fertilizing ability comparable to cells from mature adults. Microinsemination with round spermatids was performed by direct injection into preactivated oocytes, as previously reported. About 60-85% of the successfully injected oocytes developed to the morula/blastocyst stage after 72 h in culture, irrespective of the age of the males (17-25 days old). After embryo transfer, normal pups were obtained from all age groups, including the day-17 group, the stage at which the first round spermatids appeared. A high correlation (r=0.90) was found between the birth rate and male age (P<0.01, Spearman rank correlation), indicating that the efficiency of producing offspring was dependent on the age of the donor males. Imprinted genes (H19, Igf2, Meg3, and Igf2r) were expressed from the correct parental alleles (maternal, paternal, maternal, and maternal, respectively) in all (n=12) day-9.5 fetuses derived from day-20 spermatids. These results clearly indicate that at least some first-wave spermatogenic cells have a normal haploid genome with the correct paternal imprint and are capable of supporting full-term embryo development, as do mature spermatozoa from adults. The use of male germ cells from immature animals may save time in the production of inbred/congenic strains and rescue male-factor infertility of early onset. 相似文献
60.
Nakanishi T Ishibashi N Kubota H Inoue K Ogonuki N Ogura A Kashiwabara S Baba T 《The Journal of reproduction and development》2008,54(4):244-249
Sperm-specific phospholipase C, PLCzeta, is a candidate for the Ca(2+) oscillation-inducing factor that is introduced into the ooplasm upon sperm-egg fusion. In addition to the 647-residue full-length PLCzeta, s-PLCzeta lacking the N-terminal 110 amino acids is known to be present in the mouse testis. In this study, we attempted to obtain full-term offspring from s-PLCzeta-activated eggs by round spermatid injection. Metaphase II-arrested eggs injected with a high RNA concentration of s-PLCzeta RNA normally developed to blastocysts. When the round spermatid nucleus was injected into telophase II-stage eggs previously activated by s-PLCzeta RNA, three live offspring were successfully obtained by transfer of the developed 4-cell embryos to pseudopregnant mice. These three offspring all grew to be normal adults and reproduced healthy second-generation mice. 相似文献