The effects of rates of nitrogen application on seven perennial ryegrass varieties

The dry matter and crude protein production of the perennial ryegrass varieties S24, S23, Premo, Houba, Barlenna, Melle and Sceempter Pasture at annual nitrogen application rates of 212, 425, 637 and 1062 kg ha^?1 were studied for the years 1972–74 inclusive. The DM and CP response curves for the variety Melle were steeper than those for other varieties tested and levelled off at a higher N application rate. The results suggest that Melle is a variety with a genotype capahle of utilizing N more efficiently at higher rates than the other varieties tested.     

Supplemental iron and phosphorus increase dietary inclusion of cottonseed and soybean meal in olive flounder (Paralichthys olivaceus)

A long‐term feeding experiment was conducted to investigate the use of cottonseed and soybean meal (CS) with iron and phosphorus supplements in diets for olive flounder (Paralichthys olivaceus). Olive flounder with an initial average size of 28.5 ± 0.35 g (mean ± SD) were divided into 15 groups (three tanks per dietary treatment) and fed 480 g kg^?1 crude protein diets in which each of five isonitrogenous diets was formulated to contain different levels of cottonseed/soybean meal (1 : 1) to replace fish meal (FM) with iron and phosphorus supplementations. The five experimental diets were as follows: diet 1 (control), 0%CS; diet 2, 20%CS; diet 3, 30%CS; diet 4, 30%CS + Fe&P; and diet 5, 40%CS + Fe&P. After 26 weeks of feeding trial, no significant differences were observed in weight gain, feed utilization and survival among all the treatments. The total gossypol accumulation in liver of fish fed diets supplemented with iron was significantly lower than that of fish fed diets without supplementation of iron. The results indicate that the addition of iron in diets could prevent the absorption of free gossypol. The findings in this study suggest that dietary supplements of iron and phosphorus could increase the inclusion of cottonseed and soybean meal for FM replacement in diets for marine fish species.     

当北极不再是北极

北极在许多人心中意味着遥远、寒冷和神秘，是一个冰天雪地的童话世界。然而，近年来气候变暖和油气田开发对北极带来了很大冲击，当地特有的景观、传统和生活方式随着冰雪的消融在慢慢发生变化。     

The use of the green fluorescent protein as a biomarker for sapstain fungi

To understand wood colonization by sapstain fungi and their potential biocontrol agents, it is necessary to differentiate these organisms directly on their natural substrates. In the present study the feasibility of transforming with the green fluorescent protein (GFP), the sapstain fungus Ophiostoma piceae and a potential biocontrol agent Cartapip^®, an Ophiostoma piliferum albino strain was assessed. Transformants of the two fungal species were screened by polymerase chain reaction and Southern blot analyses. The GFP was expressed in spores, synnemata and mycelia of the transformants grown in artificial media or wood. The growth, pigmentation and wood colonization of the transformants were similar to that of the non‐transformants, suggesting that the presence of the gfp gene had no negative effect on the biology of the transformants. Using fluorescence and confocal microscopy, the GFP‐expressing fungi were easily differentiated from the wild‐type strains and other fungal species in wood, even 4 months after inoculation. The results show that the use of the GFP system is feasible to monitor Ophiostoma fungi in wood.     

花尾榛鸡(Bonasa bonasia)在韩国温带林中的季节性移动特征(英文)

This study were carried out in the Experimental Forest (37( 48' 10" N, 127( 48' 50" E) of Gangwon Forest Development Institute, Gamjeong-ri, Chuncheon, Gangwon-do Province, Korea from Dec. 1999 to Jul. 2002. Eight individuals (three males and five females) of hazel grouse were captured and they were marked with a 14-g necklace-type transmitter. The surveying results showed that females were more active than males throughout the year, but males were more mobile than females in spring. The degree of movement for females and males was similar from summer to winter. The overlap degree of habitat was very large from spring to autumn. Hazel grouse had greater shifts in area use in winter. They used similar area from spring to autumn, made a shift in their habitat use in winter, and then shifted back to the previous habitat.     

Establishment of optimal scan delay for multi-phase computed tomography using bolus-tracking technique in canine pancreas

To establish a protocol for a multi-phase computed tomography (CT) of the canine pancreas using the bolus-tracking technique, dynamic scan and multi-phase CT were performed in six normal beagle dogs. The dynamic scan was performed for 60 sec at 1-sec intervals after the injection (4 ml/sec) of a contrast medium, and intervals from aortic enhancement appearance to aortic, pancreatic parenchymal and portal vein peaks were measured. The multi-phase CT with 3 phases was performed three times using a bolus-tracking technique. Scan delays were 0, 15 and 30 in first multi-phase scan; 5, 20 and 35 in second multi-phase scan; and 10, 25 and 40 sec in third multi-phase scan, respectively. Attenuation values and contrast enhancement pattern were analyzed from the aorta, pancreas and portal vein. The intervals from aortic enhancement appearance to aortic, pancreatic parenchymal and portal vein peaks were 3.8 ± 0.7, 8.7 ± 0.9 and 13.3 ± 1.5 sec, respectively. The maximum attenuation values of the aorta, pancreatic parenchyma and portal vein were present at scan sections with no scan delay, a 5-sec delay and a 10-sec delay, respectively. When a multi-phase CT of the canine pancreas is triggered at aortic enhancement appearance using a bolus-tracking technique, the recommended optimal delay times of the arterial and pancreatic parenchymal phases are no scan delay and 5 sec, respectively.     

Mdivi-1, mitochondrial fission inhibitor,impairs developmental competence and mitochondrial function of embryos and cells in pigs

Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effects of mitochondrial fission inhibitor, mdivi-1, on developmental competence and mitochondrial function of porcine embryos and primary cells. Presumptive porcine embryos were cultured in PZM-3 medium supplemented with mdivi-1 (0, 10 and 50 μM) for 6 days. Porcine fibroblast cells were cultured in growth medium with mdivi-1 (0 and 50 μM) for 2 days. Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < 0.05). Moreover, loss of mitochondrial membrane potential in the mdivi-1 (50 μM) treated group was increased relative to the control group (P < 0.05). Subsequent evaluation revealed that the intracellular levels of reactive oxygen species (ROS) and the apoptotic index were increased by mdivi-1 (50 μM) treatment (P < 0.05). Finally, the expression of mitochondrial fission-related protein (Drp 1) was lower in the embryos and cells in the mdivi-1-treated group than the control group. Taken together, these results indicate that mdivi-1 treatment may inhibit developmental competence and mitochondrial function in porcine embryos and primary cells.     

近红外高光谱成像结合特征波长筛选识别小麦赤霉病瘪粒

为实现小麦赤霉病瘪粒快速识别,本研究使用主成分分析(Principal component analysis,PCA)结合最大类间方差法(Otsu)对小麦高光谱图像进行背景分割,以赤霉病瘪粒识别正确率为评价指标,探究判别分析方法与竞争性自适应权重取样法(Competitive adaptive reweighted sampling,CARS)的最佳组合方式.结果显示,基于全谱段构建的偏最小二乘判别分析(Partial least squares discrimination analysis,PLS-DA)和支持向量机判别分析(Sup-port vector machine discriminant analysis,SVM-DA)模型预测精度相同,外部验证集健康籽粒和赤霉病瘪粒识别正确率分别为95.2％和100.0％;基于CARS筛选的8个特征波长构建的CARS-PLS-DA模型外部验证集健康籽粒和赤霉病瘪粒识别正确率均为100.0％,预测精度高于CARS-SVM-DA模型,可有效实现赤霉病瘪粒的快速识别.研究结果将为谷物仓储和加工过程中赤霉病瘪粒高通量快速识别提供理论依据和技术支撑.