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排序方式: 共有174条查询结果,搜索用时 31 毫秒
131.
Mai MINAMI Takeo YAMAKAWA Akihiro YAMAMOTO Shoichiro AKAO Yuichi SAEKI 《Soil Science and Plant Nutrition》2009,55(1):65-72
The nodulation tendency of indigenous soybean bradyrhizobia on Rj -genotype soybean cultivars was investigated using approximately 260 bradyrhizobia isolated from an Andosol with 13 soybean cultivars of five Rj -genotypes (non- Rj , Rj 2 Rj 3 , Rj 3 , Rj 4 and Rj 2 Rj 3 Rj 4 ). A dendrogram was constructed based on restriction fragment length polymorphism analysis of the polymerase chain reaction products (PCR-RFLP) of the 16S–23S rDNA internal transcribed spacer (ITS) region. Bradyrhizobium USDA strains were used as a reference. The dendrogram indicated nine clusters based on similarities among the reference strains. The ratio of beta diversity to gamma diversity ( H' β / H' γ ), which represents differences in the bradyrhizobial communities by pair-wise comparison between each cultivar, was obtained from Shannon–Wiener diversity indices. The results showed that bradyrhizobial communities among the same Rj -genotype cultivars were similar to each other, whereas bradyrhizobial communities between the Rj 2 -genotype and non- Rj , Rj 3 or Rj 4 -genotype cultivars were significantly different. These results suggest that the Rj 2 -gene might not only affect the nodulation compatibility between Rj -genotype soybeans and bradyrhizobia, but also the nodulation tendency of the bradyrhizobia. 相似文献
132.
133.
Kaori Isobe Tsuneo Ito Shun-ichiro Komatsu Kentaro Asanuma Etsuko Fujii Chie Kato Kenji Adachi Atsuhiko Kato Tetsuro Sugimoto Masami Suzuki 《Journal of toxicologic pathology》2012,25(4):281-285
Increased incidence of adrenal pheochromocytoma is frequently encountered in rat
carcinogenicity studies. In some of the studies, the finding is judged to be due to a
rat-specific mechanism of carcinogenesis caused by a disturbance of calcium homeostasis.
However, direct evidence that the proliferation of chromaffin cells in the adrenal medulla is
induced solely by hypercalcemia is not available. In this study, calcium gluconate was
intravenously infused for 7 days to rat chromaffin cells by a tail cuff method, and cumulative
labeling with bromodeoxyuridine (BrdU) was carried out to evaluate the proliferative activity.
The serum calcium concentration was dose-dependently increased, and a high calcium
concentration was stably sustained from day 2 to 7. In the adrenal medulla, BrdU-positive
chromaffin cells increased in the calcium gluconate-treated animals, and the BrdU-labeling
index increased in a dose-dependent manner. In addition, an increased BrdU-labeling index of
chromaffin cells was shown to correlate with the serum calcium concentration. Our results
demonstrate that hypercalcemia directly enhances the proliferative activity of chromaffin cells
and that the proliferative activity is correlated with the serum calcium concentration. 相似文献
134.
Hara K Tsujimoto H Huang CC Kawashima Y Ando R Kusuoka O Tamura K Tsutsumi M 《Journal of toxicologic pathology》2012,25(1):19-26
Polylactide-glycolide (PLGA) nanoparticles have been developed as pulmonary drug delivery carriers. To investigate their behavior, small- (d50 = 74 nm) and large-sized (d50 = 250 nm) FITC-conjugated PLGA nanoparticles were intratracheally administered to rats and were traced for 5, 30 and 60 minutes and 24 hours after administration (HAT). Immunohistochemically, a, FITC-positive reaction was observed in type-I alveolar epithelial cells (type-I AEC), endothelial cells and alveolar macrophages in the lungs from 5 minutes after treatment (MAT) to 24 HAT in both nanoparticle groups. In the kidneys, a positive reaction was observed in proximal tubular epithelial cells at 30 MAT; the reaction peaked at 60 MAT and was reduced at 24 HAT, while no positive reaction was seen in other sites. Ultrascructurally, the number of membrane-bound vesicles, which were approximately 70 nm in size and hard to distinguish from pinocytic vesicles, apparently increased in type-I AEC and endothelial cells at 5 MAT in the small-sized group, in comparison with the control group receiving physiological saline. The number of vesicles in the large-sized group was almost same as that in the control group. On the other hand, in both nanoparticle groups, lysosomes filled with nanoparticles appeared in alveolar macrophages from 30 MAT to 24 HAT. These results indicate that PLGA nanoparticles might be quickly transferred from the alveolar space to the blood vessel via type-I alveolar epithelial cells and excreted into urine, and that there is a threshold for particle size, less than approximately 70 nm in diameter, with regard to absorption through the alveolar wall. 相似文献
135.
A novel biological activity of human recombinant interleukin-12 (rhIL-12) on canine peripheral blood mononuclear cells (PBMC) was investigated in vitro. Canine PBMC were cultured in the presence or absence of rhIL-12 for 3 days. The reactive oxygen species (ROS) production induced by opsonized-zymosan (OZ) was then measured by a luminol-dependent chemiluminescense assay and demonstrated that the ROS production was enhanced after culture with rhIL-12. A nitro blue tetrazolium test and flowcytometry analysis revealed that canine lymphocytes, eosinophils, and monocytes were capable of ROS production, but that monocytes had the highest capacity. These results suggest that rhIL-12 enhances ROS production from canine monocytes. 相似文献
136.
The absence of Rhodococcus equi in Mongolian horses 总被引:1,自引:0,他引:1
Takai S Sengee S Madarame H Hatori F Yasuoka K Ochirel E Sasaki Y Kakuda T Tsubaki S Bandi N Sodnomdarjaa R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(6):611-613
In native Mongolian horses, the incidence and distribution of Rhodococcus equi are poorly understood. One hundred and fourteen equine fecal samples and 71 soil samples were collected from the camp sites of 26 nomadic families located in three areas less than 100 km from Ulaanbaatar, Mongolia. Five fecal samples were also collected from foals of Przewalski's Horses introduced into the Hustai National Park, Mongolia. No R. equi was isolated from the Mongolian horses or the soil samples. However, three colonies of R. equi were isolated from two fecal samples collected from foals of Przewalski's Horses. These isolates were avirulent, with neither 15- to 17-kDa antigens (VapA) nor a 20-kDa antigen (VapB) genes being detected. We concluded that native Mongolian horses and their environment appear free from contamination with R. equi. 相似文献
137.
Inokuma H Fujii K Matsumoto K Okuda M Nakagome K Kosugi R Hirakawa M Onishi T 《Veterinary parasitology》2002,110(1-2):145-152
A free-roaming dog in Okinawa island showed Anaplasma (Ehrlichia) platys-like inclusions within the platelets of peripheral blood samples. The inclusions were positive in indirect fluorescence test with anti-A. phagocytophila serum. The platelet count of the dog was 170,000 microl(-1). The sequence analysis of the 16S rRNA, citrate synthase and heat shock protein genes of DNA from the infected platelets confirmed that the inclusions were A. platys. This is the first detection of A. platys inclusions in dogs in Japan. 相似文献
138.
Jun-Shan Gao Nobumitsu Sasaki Hiromi Kanegae Ken-ichi Konagaya Kaori Takizawa Naomi Hayashi Yosuke Okano Masahiro Kasahara Yasuhiko Matsushita Hiroshi Nyunoya 《Physiological and Molecular Plant Pathology》2007,71(1-3):78-87
The tobacco N protein recognizes the helicase domain (p50) of the Tobacco mosaic virus (TMV) replicase as an elicitor and mediates hypersensitive response (HR). We obtained two cDNA clones encoding novel N-like (NL) proteins NL-C26 and NL-B69 from Nicotiana tabacum cv. Samsun NN. NL-C26 and NL-B69 had a Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR–NBS–LRR) structure and showed 78% and 73% identities to N, respectively. The NL-C26 and NL-B69 genes were also expressed in N. tabacum cv. Samsun nn, which lacks the N gene. Unlike N, NL-C26 and NL-B69, when coexpressed with p50, failed to induce HR on the sites of agroinfiltration in Samsun nn leaves. However, the elicitor-dependent HR in Samsun nn was induced efficiently by chimeric N proteins with the continuous TIR–NBS domains of NL-C26 and NL-B69. On the other hand, the efficiency of HR induction varied significantly among chimeric N proteins with either of the TIR and NBS domains of the NL proteins. In contrast, chimeras carrying the LRR domains of the NL proteins did not induce HR. Thus, the TIR–NBS domains of NL-C26 and NL-B69 could functionally adapt to the LRR domain of N, which may determine the specificity for the elicitor. We speculate that the NL genes are potential HR-inducing resistance genes for undetermined pathogens other than TMV. 相似文献
139.
Isobe K Matsunaga S Nakayama H Uetsuka K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(10):1125-1128
A 1-year-and-3-month-old, male standard poodle dog with intrahepatic portosystemic shunt (PSS) was autopsied. Nineteen regions of the liver were prepared for detailed examination, and the distribution of hepatic lesions caused by PSS was studied in the liver of this dog. Histopathologically, the liver revealed a variety of hepatic lesions including lipogranulomas in the hepatic parenchyma, and a ductular reaction and microvascular proliferation in portal areas. The distribution of the lesions was not significantly different among liver regions. It is concluded that, in the present case, hepatic lesions caused by PSS are independent of shunt location, and are distributed equally in the liver. 相似文献
140.
Nucleotide sequence analysis of VP2 hypervariable domain of infectious bursal disease virus detected in Japan from 1993 to 2004 总被引:2,自引:0,他引:2