地下滴灌对棉花生理性状及产量影响的研究

根据新疆生产建设兵团农五师90团在棉花上应用地下滴灌的实践，以膜下滴灌棉花为对照，研究分析了地下滴灌对新疆棉花生理性状及产量的影响，认为地下滴灌条件下的棉花生育期有所提前、株高较低、铃数较多、叶面积系数较大、根冠比较大、根系发达并有两个根系层，生物学产量和经济产量均比较高。     

对橡胶悬架装置动特性的改进探讨

以橡胶悬架装置为研究对象,考虑其动态特性与高速车辆动力总成低频与高频要求不同之间的矛盾,试图通过改进橡胶悬架装置的结构提高其动特性。建立了改进结构的橡胶悬架装置的力学模型,并对其进行了理论分析和仿真计算,结果表明改进后的橡胶悬架装置的动特性有了显著提高,对我国橡胶悬架装置的结构改进具有借鉴和指导意义。     

电偶腐蚀和缝隙腐蚀的发生及预防

主要介绍了电偶腐蚀和缝隙腐蚀的定义、发生原理、常见形式及相应的预防措施。     

银耳子实体及菌丝体胶质化产生的双核酵母状孢子萌发条件探索

为探索由银耳(Tremella fuciformis)子实体、菌丝体胶质化产生的双核酵母状孢子(FBMds)的萌发条件,研究比较了不同来源和不同浓度的香灰菌(Annuloypoxylon stygium)浸提液及不同来源银耳孢子发酵液对银耳Tr21 FBMds萌发率的影响。试验结果表明,香灰菌浸提液和银耳孢子发酵液均有不同程度促银耳Tr21FBMds萌发的作用。不同浓度香灰菌浸提液对FBMds萌发率的试验表明,在一定浓度范围内,高浓度香灰菌浸提液可显著提高银耳FBMds萌发率,最佳浓度为40%;在该浓度下,银耳Tr21的原伴生香灰菌HTW01-5的浸提液对FBMds萌发率作用高达30.34%。银耳孢子T30-CAS、Tr01、Tr21、T8225-CAS的发酵液虽能显著促进银耳Tr21 FBMds的萌发,但效果不如香灰菌浸提液明显。研究所得银耳菌丝为纯菌丝,可稳定传代,为进一步研究银耳二型态转变以及银耳新品种选育奠定了扎实基础。     

锦橙园叶面补充镁肥控制大小年的效果

在重庆市开州区长沙镇选择栽培品种、砧木、定植时间一致的2个果园分别作为对照果园和处理果园。对照果园每年仅土壤施肥3次,株施氮磷钾比例为10-4-8的有机无机复合肥3 kg,分别于3月底、6月底和9月底施0.6、0.6和1.8 kg。处理果园土壤施肥同对照果园,同时根据上一年叶片营养诊断结果进行叶面补镁肥,若缺镁,则分别在5月上旬、5月中旬、9月中旬3次进行叶片喷施0.2%七水硫酸镁。调查结果表明,对照果园叶片镁含量长期处于偏低水平,历年平均叶片缺镁症率4.33%。处理果园叶面喷施0.2%七水硫酸镁可以显著降低大小年结果的产量差和提高单产,7年内产量差为392.86 kg,较对照低19.58%,平均年产量1 860.25 kg/667 m~2,较对照高38.15%,叶片缺镁症率控制在1.58%,较对照低59.69%。     

CD147 monoclonal antibody-mediated nanoparticles for gene therapy to target lung cancer cells

AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells.     

河北昌黎产区酿酒葡萄“赤霞珠”霜霉病发生情况调查

以酿酒葡萄"赤霞珠"为试验材料,调查了2012—2014年昌黎产区酿酒葡萄"赤霞珠"霜霉病的发生情况。结果表明:葡萄霜霉病在昌黎产区一般从6月下旬开始出现,7—8月是发病高峰期;对葡萄霜霉病的病情指数和气象因子进行相关分析表明,温度、降雨量及空气相对湿度对葡萄霜霉病发生影响较大,昼夜温差和日照时数对该病的发生没有显著影响。     

关于光学系统光心的商榷

文章主要探讨和阐述了光学系统中的光心如何确定、如何定义、如何计算,并明确指出了确定光心的重要性和实际意义.     

SPF雏鸡感染REV后IL-2 mRNA表达的动态变化

为研究SPF鸡感染禽网状内皮组织增生症病毒(REV)后白细胞介素2(IL-2) mRNA表达的动态变化,本研究应用荧光定量RT-PCR方法,对SPF鸡感染REV后主要免疫器官的IL-2 mRNA转录水平进行了初步研究.结果显示,与对照组相比,REV感染组鸡的脾脏、胸腺和法氏囊中IL-2基因的表达量在感染后7d、14d、21d、28 d、35 d、42 d和49 d均呈现升高,而且除21日龄、28日龄鸡的脾脏外,均为差异显著(p＜0.05).本研究表明REV感染后可以诱导鸡的机体表达IL-2.     

Cloning and analysis of cross-intron genomic gene encoding ACO in Paeonia suffruticosa Andr.

The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by ACC oxidase (ACO). In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO (FJ855434) has 1281 bases, containing four exons and three introns, encoding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.