Impact of Nutrient Seed Priming on Germination,Seedling Development,Nutritional Status and Grain Yield of Maize

Effects of seed priming with zinc (Zn) plus manganese (Mn), boron (B), and phosphate (P) on growth and nutritional status of maize were studied. Nutrient seed priming significantly increased seed contents of primed nutrients. In nutrient solution (NS) lacking Zn and Mn, growth of maize plants primed with Zn?+?Mn increased by more than 50% and 100%, respectively, as compared to control treatment. The primed nutrients were efficiently translocated to the growing shoot and could maintain Zn and Mn supply for at least three weeks of the culture period. In soil culture, plants suffered from P and Zn deficiency, which was mitigated to some extent by P and Zn?+?Mn priming. Particularly, translocation of Zn seed reserves to the shoot tissue was negatively affected by the highly calcareous soil. In the field experiment, Zn?+?Mn seed priming increased grain yield by 15%, demonstrating the potential for long-lasting effects of nutrient seed priming.     

Effects of Pseudomonas aeruginosa on the diversity of culturable microfungi and nematodes associated with tomato: impact on root-knot disease and plant growth

The available information on Pseudomonas biocontrol inoculants on the non-target fungal and nematode community is scant. The current paper addresses this issue and investigates the effects of biocontrol agents Pseudomonas aeruginosa IE-6 and IE-6S⁺ (previously shown to suppress several soil-borne plant pathogens) on soil microfungi and plant-parasitic nematodes as well as on the root-knot development and growth of tomato (Lycopersicon esculentum). Furadan, a granular nematicide was included as a treatment for comparative purposes. Treatments were applied to soil at the start of each 52-day-long tomato growth cycle, and their effects on the composition and diversity of rhizosphere and endophytic microfungi and plant-parasitic nematodes were examined at the end of first and fourth growth cycle. Several diversity indices were employed to assess community diversity. A total of 16 genera comprising 23 microfungal species were isolated from the tomato rhizosphere. The most abundant fungal species belonged to the genera Aspergillus, Fusarium, and Penicillium. With a few exceptions, fungi were neither exclusively inhibited nor specifically promoted by the application of treatments at any of the growth cycles studied. However, Paecilomyces lilacinus, an egg and female parasite of root-knot nematode, though exclusively absent in the controls was isolated from the treatments. Both general diversity and equitability of rhizosphere microfungi were greater at first compared to the fourth growth cycle while species richness remained uninfluenced across the growth cycles and treatments. However, Furadan and IE-6S⁺ treatments considerably abated general diversity and equitability. Of the microfungal species isolated from the rhizosphere seven were also recovered from surface-sterilized root tissue of tomato suggesting that all the endophytes are primarily rhizosphere organisms. Diversity of endophytic fungi was consistently lower compared with that of the rhizosphere. Both general diversity and equitability declined in all three treatments relative to controls in the root tissue but species richness remained unaltered. Diversity and equitability of plant-parasitic nematodes in soil were reduced by all three treatments over the controls at fourth growth cycle whilst species richness did not change at either growth cycle. The biocontrol agents significantly reduced root-knot development and enhanced shoot growth of tomato over the controls. The possible implications of fungal composition and abundance because of biocontrol by Pseudomonas application are discussed.     

Assessment of phenotypic and molecular diversity in soybean [Glycine max (L.) Merr.] germplasm using morpho-biochemical attributes and SSR markers

Genetic Resources and Crop Evolution - Soybean is a high-nutritional crop that provides a sustainable source of dietary protein and edible oil for human consumption and animal diet. In this...     

Effect of salicylic acid (SA) on delaying fruit senescence of Huang Kum pear

This experiment was undertaken to explore the effect of salicylic acid (SA) at different concentrations on regulating fruit senescence of Huang Kum pear. Through dipping fruits and fruit discs for a series of hours in SA solution, enzyme activities and physiological characteristics of Huang Kum pear were determined. The results revealed that SA enhanced the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes at 0.02 mmol/L and at 0.002 mmol/L with the treatment of dipping fruit discs for 4 h and 12 h, respectively. The malondialdehyde (MDA) contents were reduced at 0.002 mmol/L for 12 h, and water loss ratio was decreased at 0.5 mmol/L after 48 h of treatment. It was concluded that SA at lower concentrations could delay the senescence of Huang Kum pear fruit.     

Assessment of Chemical and Manual Weed Control Approaches for Effective Weed Suppression and Maize Productivity Enhancement Under Maize-Wheat Cropping System

Effective weed management in maize-wheat (M-W) cropping systems are accountable for higher yield and plant biomass. The objective of this study was to evaluate the effectiveness of manual weeding and chemicals (pre- and post-emergence herbicide) for actual weed control to enhance maize yield and M?W system productivity. Herbicides were treated as pre-emergence (sole), post-emergence (sole) and its combined application (pre- and post-emergence) for weed eradication and suppression. Sole pre-emergence herbicide (acetyclour?+?atrazine) and sole post-emergence herbicides (nicosulfuron), and its mixed application were used in the experiments. The experiment was comprised on 6 treatments with one weedy check or control plot: W1?=?weedy check, W2?=?acetyclour?+?atrazine, W3?=?atrazine, W4?=?nicosulfuron, W5?=?nicosulfuron?+?atrazine, W6?=?acetyclour?+?atrazine?+?nicosulfuron and W7?=?manual weeding. Main weeds were Cyperus rotundus, Tribulus terrestris, Dactyloctenium aegyptium and Cynodon dactylon. Experiential findings revealed that manual weeding was found most effective in terms of controlling weed and suppression. Among the herbicides application, promising results in terms of weed destruction and suppression were shown by combined application of acetyclour?+?atrazine?+?nicosulfuron which effectively controlled weeds as compared to other treatments. It was concluded that manual weeding drastically reduced weeds frequency and biomass while among the herbicides application acetyclour?+?atrazine and nicosulfuron could be promising for weeds suppression and clamp downing as compared to all other treatments under M?W cropping system.

     

A study on the synergetic effect of Bacillus amyloliquefaciens and dipotassium phosphate on Alternaria solani causing early blight disease of tomato

European Journal of Plant Pathology - A study of the effect of bioagents and dipotassium phosphate (DPP) and their combination on early blight disease reduction under greenhouse conditions was...     

EFFECT OF DIFFERENT PHOSPHORUS SOURCES ON THE GROWTH,YIELD, ENERGY CONTENT AND PHOSPHORUS UTILIZATION EFFICIENCY IN MAIZE AT RAWALAKOT AZAD JAMMU AND KASHMIR,PAKISTAN

Effect of poultry manure (PM) and four inorganic phosphorus (P) fertilizers sources, i.e., diammonium phosphate (DAP), single super phosphate (SSP), nitrophos (NP) and triple super phosphate (TSP) on crop production and P utilization efficiency (PUE) of maize was studied. Both inorganic P fertilizers and PM applied alone or combined in 50:50 proportions at equivalent rate of 90 kg P₂O₅ ha^?1. Results indicated that inorganic P sources with PM significantly increased plant height, leaf area and chlorophyll content. Average values showed that combined application of inorganic P with PM increased grain yield by 19 and 41% over inorganic P and PM alone, respectively. Similarly, increase in P-uptake due to the combined application of inorganic P + PM was 17% compared to sole inorganic P. Phosphorus utilization efficiency of inorganic P was increased with PM and the highest PUE was recorded in DAP + PM. Generally, combination of DAP + PM proved superior over the remaining P fertilizers.     

Investigating the effect of Azospirillum brasilense and Rhizobium pisi on agronomic traits of wheat (Triticum aestivum L.)

ABSTRACT

Plant growth-promoting rhizobacteria (PGPR) play an important role in improving crop growth but have not been studied sufficiently. A wire house experiment was conducted in Pakistan to determine the combined effect of inoculating wheat seeds with PGPR on the subsequent growth and yield of the wheat. The experiment included four treatments: T_{0 –} no-inoculation (control), T₁ – Azospirillum brasilense inoculation, T₂ – Rhizobium pisi inoculation and T₃ – co-inoculation with A. brasilense and R. pisi. Development and growth attributes, as well as final yield of wheat, were studied. Co-inoculation of seeds with both strains increased significantly wheat grain yield, the number of grains per plant and 1000-grain weight by 36%, 11% and 17%, respectively, compared to non-inoculated control. While crop growth rate increased for, respectively, 5.5% and 33% at tillering and flag leaf stages, corresponding values for T₃ were about 9% and 14% higher than values for sole inoculations in T₁ and T₂. Co-inoculation also significantly increased leaf epicuticular wax and relative water content as compared to the control treatment. Thus, inoculation of wheat seeds with A. brasilense and R. pisi and their combination is a promising method to improve growth, yield and quality of wheat.     

The possible role of organic acids as allelochemicals in Tamarindus indica L. leaves

Tamarindus indica L. is well known for its acidic nature and allelopathic potential, but to date, little is known about its organic acids playing their role as allelochemicals. Hence, in the present study, identification, quantification, and contribution of organic acids present in its leaf extract were conducted using the principle of bioassay-guided procedure. High pressure liquid chromatography identified four organic acids, viz. citric, malic, oxalic, and tartaric acids, in its leaf aqueous extract with the predominance of oxalic acid (7.5 g kg^?1 leaves fresh weight) followed by tartaric acid (7.3 g kg^?1). The allelopathic activity of identified acids and aqueous extract was evaluated on lettuce seedlings growth based on the specific activity (EC₅₀). The crude extract reduced radicle growth more adversely than hypocotyl at the concentration of 2.5 g L^?1 (EC₅₀). It hindered the normal physiological growth process through weak and curly seedlings, and necrosis of their tips. Among the identified acids, oxalic acid had the highest specific activity (40 mg L^?1) and citric acid had the lowest (>1000 mg L^?1). As a consequence of its high contents, the total activity, a function of specific activity and concentration, of oxalic acid (188) was found higher followed by tartaric acid (146). The contribution of both acids influencing the specific activity of the crude extract was then turned out to be 74%. To the best of our knowledge, this is the first report identifying the oxalic and tartaric acids as growth inhibitors in tamarind leaves and quantifying their contribution in its allelopathic expression. Based on the total activity, the results suggested that oxalic and tartaric acids are the major allelochemicals in tamarind leaves. The allelopathic potential of these acids might promote the development of natural herbicides as an alternative to the synthetic ones in a most sustainable manner.