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51.
Marcelino Herrera Luis Vargas-Chacoff Ismael Hachero Ignacio Ruíz-Jarabo Ana Rodiles José I. Navas & Juan M. Mancera 《Aquaculture Research》2009,40(7):790-797
Physiological responses to a high stocking density were tested in juvenile wedge sole Dicologoglossa cuneata (Moreau). Fish were kept at low (1 kg m−2 ), medium (3 kg m−2 ) and high (9 kg m−2 ) stocking densities for 22 days. No differences in the weight, length, survival and hepatosomatic index were observed among treatments. Basal plasma cortisol and osmolality were found to be directly and positively related to stocking density. A mild increase in plasma glucose was seen at medium density, and plasma protein was elevated at medium and high densities. The liver glucose and glycogen content was inversely related to stocking density. The liver triglyceride level was significantly elevated at the highest density, and the α-amino acid content decreased at the highest density. In muscle, glucose levels were significantly higher in fish kept at the lowest density; the α-amino acid content was elevated in fish kept at high density. In conclusion, plasma cortisol levels indicated an increasing stress level depending on the culture density, but significant changes in energy reserves did not occur in tissue (mainly liver and muscle glycogen and glucose reserves were significantly affected). 相似文献
52.
Reproductive resilience: a paradigm shift in understanding spawner‐recruit systems in exploited marine fish 下载免费PDF全文
Susan Lowerre‐Barbieri Greg DeCelles Pierre Pepin Ignacio A Catalán Barbara Muhling Brad Erisman Steven X Cadrin Josep Alós Andres Ospina‐Alvarez Megan M Stachura Michael D Tringali Sarah Walters Burnsed Claire B Paris 《Fish and Fisheries》2017,18(2):285-312
A close relationship between adult abundance and stock productivity may not exist for many marine fish stocks, resulting in concern that the management goal of maximum sustainable yield is either inefficient or risky. Although reproductive success is tightly coupled with adult abundance and fecundity in many terrestrial animals, in exploited marine fish where and when fish spawn and consequent dispersal dynamics may have a greater impact. Here, we propose an eco‐evolutionary perspective, reproductive resilience, to understand connectivity and productivity in marine fish. Reproductive resilience is the capacity of a population to maintain the reproductive success needed to result in long‐term population stability despite disturbances. A stock's reproductive resilience is driven by the underlying traits in its spawner‐recruit system, selected for over evolutionary timescales, and the ecological context within which it is operating. Spawner‐recruit systems are species specific, have both density‐dependent and fitness feedback loops and are made up of fixed, behavioural and ecologically variable traits. They operate over multiple temporal, spatial and biological scales, with trait diversity affecting reproductive resilience at both the population and individual (i.e. portfolio) scales. Models of spawner‐recruit systems fall within three categories: (i) two‐dimensional models (i.e. spawner and recruit); (ii) process‐based biophysical dispersal models which integrate physical and environmental processes into understanding recruitment; and (iii) complex spatially explicit integrated life cycle models. We review these models and their underlying assumptions about reproductive success vs. our emerging mechanistic understanding. We conclude with practical guidelines for integrating reproductive resilience into assessments of population connectivity and stock productivity. 相似文献
53.
Katherinne Valderrama Manuel Soto‐Dvila Cristopher Segovia Ignacio Vsquez My Dang Javier Santander 《Journal of fish diseases》2019,42(11):1601-1608
Aeromonas salmonicida subsp. salmonicida (hereafter A. salmonicida) is the aetiological agent of furunculosis in marine and freshwater fish. Once A. salmonicida invade the fish host through skin, gut or gills, it spreads and colonizes the head kidney, liver, spleen and brain. A. salmonicida infects leucocytes and exhibits an extracellular phase in the blood of the host; however, it is unknown whether A. salmonicida have an intraerythrocytic phase. Here, we evaluate whether A. salmonicida infects Atlantic salmon (Salmo salar) erythrocytes in vitro and in vivo. A. salmonicida did not kill primary S. salar erythrocytes, even in the presence of high bacterial loads, but A. salmonicida invaded the S. salar erythrocytes in the absence of evident haemolysis. Naïve Atlantic salmon smolts intraperitoneally infected with A. salmonicida showed bacteraemia 5 days post‐infection and the presence of intraerythrocytic A. salmonicida. Our results reveal a novel intraerythrocytic phase during A. salmonicida infection. 相似文献
54.
Salwinder S. Dhaliwal Gurpal S. Toor Ignacio A. Rodriguez-Jorquera Todd Z. Osborne Susan Newman 《Journal of Soils and Sediments》2018,18(2):342-351
Purpose
Inorganic contaminants present a major challenge for the restoration of aquatic ecosystems. The objectives of this study were to determine the extent of trace metal contamination and investigate the influence of different plant communities on trace metal accumulation in the soils of the Florida Everglades.Materials and methods
Soil samples (n?=?117) were collected from 0 to 10-cm depth using a stainless steel coring device from sites with three dominant plant communities—cattail, sawgrass, and slough—of Water Conservation Area-2A (43,281 ha) of Florida Everglades.Results and discussion
The mean pH in soils collected from three plant communities was 6.75–6.82, whereas electrical conductivity was slightly greater in the sawgrass (0.69 dS m?1) than cattail (0.58 dS m?1) and slough (0.40 dS m?1). Mean reduction–oxidation potential was greatest in cattail (?113 mV) than sawgrass (?85.3 mV) and slough (?48.3 mV) soils. Among 11 trace metals (As, B, Co, Cr, Cu, Mn, Mo, Na, Ni, Pb, Zn) found in soil samples, Na had the greatest contents and was greater in cattail (2070 mg kg?1) and sawgrass (1735 mg kg?1) than slough (1297 mg kg?1). Four trace metals (B, Cu, Mo, Ni) were significantly greater in cattail than sawgrass and slough. Whereas, Mn was significantly lower in cattail (31 mg kg?1) than both sawgrass (84 mg kg?1) and slough (51 mg kg?1). Cattail also had significantly lower Cr (1.97 mg kg?1) and Pb (10 mg kg?1) than sawgrass (Cr 2.5 mg kg?1; Pb 20.8 mg kg?1). As (<6.9 mg kg?1), Co (<1.3 mg kg?1), and Zn (<17.2 mg kg?1) were not significantly different among soils collected from three plant community-dominant sites. Contents of Cd and Se were below the method detection limits (Cd 0.01 mg L?1; Se 0.2 mg L?1) and are not reported.Conclusions
None of the trace metals in the soils exceeded the US Environmental Protection Agency sediment toxicity thresholds. Results from this study provided baseline concentrations of trace metals, which can be used to measure the success of restoration efforts in Florida Everglades.55.
Accumulation and depletion of soil phosphorus (P) was studied in a long‐term (37 y) field experiment in Southern Finland. The loam soil had a high pH (7.5–7.7) due to an earlier liming. Spring barley, spring wheat, oat, and ryegrass, grown in rotation, were annually fertilized with 0, 32, or 67 kg P ha?1 y?1 (P0, P1, and P2K) and sufficient N. The average dry matter grain yield 2,600 kg ha?1 of the P0 plots increased by about 500 kg ha?1 at P1 treatment and another 600 kg ha?1 by P2K. Soil samples were collected in 1978 (beginning), 1995, 2005, and 2015. According to the Chang and Jackson sequential extraction, the P2K and P1 treatments increased the inorganic soil P by 732 and 32 kg P ha ?1 in 37 years, respectively, while the P0 plots were depleted by –459 kg P ha ?1. The P2K treatment increased all four P fractions, extracted with NH4Cl (easily soluble), NH4F (Al‐P), NaOH (Fe‐P), and H2SO4 (Ca‐P). Continuous depletion (P0) decreased the NH4Cl‐P and NH4F‐P pools, NaOH‐P and H2SO4‐P pools remaining stable. None of the P pools changed significantly at P1. The remarkable gap between the measured change and the balance for the P2K and P1 treatments cannot be explained solely by lateral soil movement, meaning that a significant proportion of the applied P was lost either in surface runoff or transported below the investigated depth of 40 cm. Despite large P applications, the degree of P saturation reached only 20% in the P2K topsoil, assuming a 50% reactivity of Fe and Al oxides. As derived from sorption isotherms, a high EPC0 (i.e., equilibrium P concentration at zero net P sorption or desorption) of 1.30 mg L?1 had been built up in the P2K treatment, while in the P1 treatment EPC0 (0.33 mg L?1) had remained unchanged and P depletion (P0) had caused a decrease to 0.12 mg L?1. These results demonstrate that P sorption and desorption properties respond strongly to both P fertilization and null fertilization treatments and that in a long‐term field experiment only a low proportion of the residual fertilizer P can be recovered from soil. 相似文献
56.
Mechanisms of glyphosate resistance and response to alternative herbicide‐based management in populations of the three Conyza species introduced in southern Spain 下载免费PDF全文
57.
58.
Non‐invasive fast real‐time PCR assay for detection of the enteric parasite Enteromyxum scophthalmi in cultured turbot (Scophthalmus maximus L.) 下载免费PDF全文
Mercedes Alonso Fátima C Lago María Gómez‐Reino Jacobo Fernández Casal Iris Martín Varela Juan M Vieites Montserrat Espiñeira 《Aquaculture Research》2015,46(9):2104-2115
Enteromyxum scophthalmi is a myxozoan parasite that causes severe parasitic diseases in cultured turbot affecting mainly the intestine of the host. It is characterized by producing acute enteritis, starvation and eventually death. Current diagnosis of E. scopthalmi use traditional techniques, based on the identification of the morphology of the parasite. These techniques take extended time to be carried out and do not favour the adoption of control measure at turbot farms and require the sacrifice of fish. This study develops a fast real‐time PCR molecular tool for the detection of E. scophthalmi in infected farmed turbot. This methodology is applicable for routine controls on the farm at every stage of the parasite infection. Results of the study demonstrate the robustness, specificity, efficiency and reliability of the technique. In addition, this study also provides a non‐invasive procedure of sampling through swaps. This allows control, prevention and diagnosis of the parasite infection at turbot farms while maintaining the welfare of the cultivated fish and avoiding sacrifice of the fish sampled. 相似文献
59.
Shiren Song María del Mar Hernández Ignacio Provedo Cristina M. Menéndez 《Euphytica》2014,195(2):259-277
The main objective of this research was the evaluation of the variability present in a segregating wine grape population derived from a cross between Graciano × Tempranillo, two Spanish varieties, in order to select improved genotypes with potential for producing high-quality wines in a climate change scenario. For that purpose, the phenotypic segregation of 16 agronomic traits related to production and phenology and 11 enological traits related to technical and phenolic maturity was studied in the progeny for three consecutive years. All traits presented transgressive segregation and continuous variation. Year effect was significant for all traits except total, extractable and skin anthocyanins content. However, a high level of genotype consistency for enological traits was revealed by repeatabilities and correlations between years. Significant correlations among traits were observed but most associations were weak. Furthermore, the CAPS (Cleaved Amplified Polymorphic Sequence) marker for the VvmybA genotype was tested to determine whether it would be useful in indirect selection for berry anthocyanins content. The results showed that the number of homozygous and heterozygous genotypes for the functional colour allele adjusted to a 1:1 segregation ratio, and that homozygous genotypes had significantly higher anthocyanins content. Principal component analysis found eight variables that contributed up to 80 % of the phenotypic variability present in the population. Seven groups of hybrids were distinguished based on ripening time, cluster weight, berry weight and anthocyanins content by cluster analysis; and fourteen genotypes were pre-selected for further research. 相似文献
60.
Casas M Pujols J Rosell R de Deus N Peralta B Pina S Casal J Martín M 《Veterinary microbiology》2009,135(3-4):248-252
The objective of the present work was to ascertain the date in which hepatitis E virus (HEV) was introduced in the Spanish pig population. For this, a serological retrospective study was carried out using archived sera. A total of 2871 serum samples gathered between 1985 and 1997 and collected in 208 farms of Spain were tested for anti-HEV IgG by an in-house ELISA. Of the 2871 sera analyzed by ELISA, 1390 were positive for anti-HEV antibodies (48.4%, 95% CI: 46.9-49.9%) and that corresponded to 204/208 farms (98%, 95% CI: 96.1-99.9%) having at least one positive pig. Our results show that HEV was present and widespread in Spanish swine farms at least since 1985. Any significant changes in prevalence were detected from 1 year to another and therefore, HEV infection in swine should be considered endemic in Spain. 相似文献