Export of north american ozone pollution to the north atlantic ocean

Measurement of the levels of ozone and carbon monoxide (a tracer of anthropogenic pollution) at three surface sites on the Atlantic coast of Canada allow the estimation of the amount of ozone photochemically produced from anthropogenic precursors over North America and transported to the lower troposphere over the temperate North Atlantic Ocean. This amount is greater than that injected from the stratosphere, the primary natural source of ozone. This conclusion supports the contention that ozone derived from anthropogenic pollution has a hemisphere-wide effect at northern temperate latitudes.     

Potential for Asexual Propagation of Several Commercially Important Species of Tropical Sea Cucumber (Echinodermata)

Six species of tropical sea cucumbers (Echinodermata) of high to moderate commercial value were evaluated as to their potential for being propagated asexually by induction of transverse fission. The species considered were Thelenota ananas, Holothuria fuscogilva, Actinopyga mauritiana, A. miliaris, Stichopus chloronotus , and S. variegatus . Rubber bands placed midbody on the sea cncumbers provided an effective yet simple technique to induce transverse fission. Although fission could be induced in all six species, only T. ananas and S. chloronotus had the ability to regenerate both anterior and posterior parts into whole animals. Other species showed no or low potential for regeneration ( H. fuscogilva, A. mauritiana ) or regeneration of only the posterior part into whole animals ( S. variegatus, A. miliaris ). Both T. ananas and S. chloronotus had survivorship of nearly 80% or greater. S. chloronotus regenerated anterior and posterior parts into whole animals within 3 mo. In contrast, T. ananas regenerated the posterior part into a whole animal within 5 mo while the anterior part was regenerated within 7 mo. Consequently, weight recovery began earlier with S. chloronotus relative to T. ananas .     

Use of a laryngeal mask airway in rabbits during isoflurane anesthesia

The purpose of this study was to find out if an LMA (#1 LMA‐Classic) would provide a better airway than a face mask in spontaneously breathing anesthetized rabbits, and to test if it could be used for mechanically controlled ventilation. Sixteen rabbits (4.1 ± 0.8 kg, mean ± SD) were assigned randomly to three treatment groups; face mask with spontaneous ventilation (FM‐SV; n = 5), LMA with spontaneous ventilation (LMA‐SV; n = 5), and LMA with controlled ventilation (LMA‐CV; n = 6). Rabbits were anesthetized in dorsal recumbency using a circle circuit at constant ET isoflurane (2.3%, Datex airway gas monitor) and constant rectal temperature (38.85 °C) for 2 hours. PaCO₂, PaO₂, minute volume, tidal volume (Wright's respirometer), and Pe CO₂ were measured at 15 minute intervals. Two individuals in the FM‐SV group had PaCO₂ >100 mm Hg (>13.3 kPa). One rabbit in the FM‐SV had PaO₂ <80 mm Hg (<10.7 kPa). All FM‐SV rabbits showed signs of airway obstruction and two were withdrawn from the study at 45 and 90 minutes, respectively, because of cyanosis. Tidal volume could not be measured in the FM‐SV group. No signs of airway obstructions were observed in either of the LMA groups. Four rabbits in the LMA‐CV group developed gastric tympany, and one of these refluxed after 110 minutes. The significance of differences between the two spontaneously breathing groups and between the two LMA groups were measured using Wilcoxon's rank sum test (with significance assumed at p < 0.05). There were no statistical differences between FM‐SV and LMA‐SV in any variable tested. PaCO₂ and Pe ′CO₂ were less in the LMA‐CV group than in the LMA‐SV group, while PaO₂, tidal volume, and minute volume were all more. We conclude that biologically, the LMA provides a better airway than the face mask during spontaneous breathing and that it can be used for IPPV, but that gastric tympany is likely to occur during IPPV.     

Influence of preweaning nutrition on growth of Angus calves provided a postweaning nutritional system resulting in discontinuous growth

Postweaning growth patterns and preweaning milk and forage intake were observed over a 4-yr period for 137 Angus calves allowed either fescue-legume or fescue pastures preweaning. After weaning, calves grazed fescue pasture until spring for a slow growth phase and then fescue, orchard grass, bluegrass and ladino clover pastures for 4 mo. Following this period, the calves were confined and fed shelled corn and a 36% crude protein supplement until they reached 12 mm of fat over the 12th rib. The spring growing period and the finishing period were classified as a rapid growth phase. Calves that were fatter at weaning lost more fat, but gained more in wither height during the slow growth phase, than those that were thinner at weaning. Apparently, fatter calves were more capable of continuous skeletal growth on a low plane of nutrition because they could retrieve fat energy for this growth. In spring, calves that had been relatively fat at weaning held no advantage in fatness, but had larger frames. Therefore, during the rapid growth phase, they were able to fatten more rapidly than those that were thinner at weaning. Also, calves that were more efficient in preweaning growth had more capacity for rapid postweaning growth when restricted to relatively low quality pasture. Preweaning pasture type influenced pattern of postweaning growth. Part of this influence was associated with differences detected at weaning. An additional part was associated with efficiency of forage and milk utilization during the preweaning phase.     

Granulomatous meningoencephalomyelitis with peripheral nervous system involvement in a dog

A 9-year-old male neutered Labrador Retriever presented with signs consistent with multifocal neurological disease. Cerebrospinal fluid analysis revealed a mononuclear pleocytosis and electromyography revealed abnormal electrical activity in distal appendicular and masticatory muscles. Treatment was declined and necropsy revealed disseminated granulomatous meningoencephalomyelitis with extensive involvement of the peripheral nervous system.     

Molecular subtyping of feline immunodeficiency virus from cats in Melbourne

OBJECTIVE: To determine the subtypes of feline immunodeficiency virus (FIV) present in the domestic cat population in Melbourne. METHODS: Blood samples were collected from 42 cats that had serum antibodies against FIV. DNA was extracted and subjected to polymerase chain reaction (PCR) to amplify variable regions of the envelope (env) and group specific antigen (gag) genes of FIV. PCR products were directly sequenced or sequenced after cloning when direct sequencing yielded ambiguous results. Phylogenetic analysis was performed and comparisons made with representative sequences of different subtypes. RESULTS: The variable region of the env gene was successfully amplified by PCR from 41 of the 42 cats. All 41 were found to cluster with subtype A env sequences. The variable region of the gag gene was successfully amplified by PCR from all 42 cats. Forty-one were found to cluster with subtype A gag genes and one was found to cluster with subtype B sequences, suggesting that it may be derived from a recombinant env A/gag B virus. CONCLUSIONS: Subtype A is the predominant FIV type in Melbourne, although a subtype A/B recombinant was identified in the population of FIV positive cats. These results of env gene analysis were similar to those in a previous Australian study, suggesting that subtype A predominates in Australia. The results of the gag gene analysis show the importance of analysing multiple areas of the FIV genome when assigning FIV subtypes. Comparison with other major urban centres may provide useful information about the phylogenic diversity of FIV in Australia.     

Tracking the long-term decline and recovery of an isolated population

Effects of small population size and reduced genetic variation on the viability of wild animal populations remain controversial. During a 35-year study of a remnant population of greater prairie chickens, population size decreased from 2000 individuals in 1962 to fewer than 50 by 1994. Concurrently, both fitness, as measured by fertility and hatching rates of eggs, and genetic diversity declined significantly. Conservation measures initiated in 1992 with translocations of birds from large, genetically diverse populations restored egg viability. Thus, sufficient genetic resources appear to be critical for maintaining populations of greater prairie chickens.     

Synthesis of large arrays of well-aligned carbon nanotubes on glass

Free-standing aligned carbon nanotubes have previously been grown above 700 degreesC on mesoporous silica embedded with iron nanoparticles. Here, carbon nanotubes aligned over areas up to several square centimeters were grown on nickel-coated glass below 666 degreesC by plasma-enhanced hot filament chemical vapor deposition. Acetylene gas was used as the carbon source and ammonia gas was used as a catalyst and dilution gas. Nanotubes with controllable diameters from 20 to 400 nanometers and lengths from 0. 1 to 50 micrometers were obtained. Using this method, large panels of aligned carbon nanotubes can be made under conditions that are suitable for device fabrication.