全文获取类型
收费全文 | 277篇 |
免费 | 7篇 |
专业分类
林业 | 24篇 |
农学 | 7篇 |
21篇 | |
综合类 | 21篇 |
农作物 | 7篇 |
水产渔业 | 47篇 |
畜牧兽医 | 140篇 |
园艺 | 1篇 |
植物保护 | 16篇 |
出版年
2024年 | 1篇 |
2022年 | 4篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 6篇 |
2017年 | 2篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 6篇 |
2013年 | 18篇 |
2012年 | 11篇 |
2011年 | 18篇 |
2010年 | 6篇 |
2009年 | 8篇 |
2008年 | 20篇 |
2007年 | 11篇 |
2006年 | 18篇 |
2005年 | 24篇 |
2004年 | 8篇 |
2003年 | 10篇 |
2002年 | 8篇 |
2001年 | 16篇 |
2000年 | 8篇 |
1999年 | 5篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1993年 | 2篇 |
1992年 | 9篇 |
1991年 | 4篇 |
1990年 | 4篇 |
1989年 | 8篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1969年 | 1篇 |
1967年 | 1篇 |
1940年 | 1篇 |
排序方式: 共有284条查询结果,搜索用时 15 毫秒
101.
ABSTRACT: The low salt extract from scallop striated adductor muscle contained extra proteins in addition to sarcoplasmic proteins and was turned into a gel upon standing or immediately by the addition of Ca2+ . The amount of extra protein, which was composed of a large amount of actin and a small amount of myosin, decreased with a decrease in adenosine triphosphate (ATP) content in the muscle during storage at 5°C. Actin was easily extracted from scallop myofibrils in low salt solution with ATP at 1 mM or above. Furthermore, ATP was required to induce the gelation of the low salt extract. A visual observation of the gelation of low salt extract is therefore a simple and easy method to inspect prerigor state of scallop adductor muscle. 相似文献
102.
Masatoshi Furukawa Takashi Sakakibara Kouta Itoh Kohtaro Kawamura Satoshi Sasaki Masao Matsuura 《Journal of toxicologic pathology》2015,28(4):243-248
The bovine corneal opacity and permeability (BCOP) assay is an alternative method to the in vivo Draize eye test in rabbits for evaluating eye irritation in vitro. Here, we compared the numerical results of the BCOP assay with the corresponding histopathology for three different corneas for each test substance, including commercially available shampoos, make-up removers and cleansing foams that contained surfactants and other ingredients. The histopathological score was defined based on the severity of lesions in the corneal epithelium. The histopathological findings and scores of the three sections for each test substance were comparable. The in vitro irritancy score (IVIS) generally corresponds to the corneal irritant potential of the test substances assigned on the basis of the histopathological findings in this study. In the present study, we characterized the histopathology of the corneal epithelium and stroma and especially showed that the corneal epithelial injury caused by test substances might be important in assessment of test substances that are mild eye irritants (category 2B) as classified by the United Nations (UN) Globally Harmonized System of Classification and Labelling of Chemicals (GHS), as corneal lesions suggestive of classification into category 2B were localized on the border between the corneal epithelium and stroma, which contained cell elements related to assessment of prognosis of an in vivo eye injury. Histopathological assessment might be useful in predicting in vivo ocular irritation, particularly for test substances with an IVIS >3.1 but ≤25 that are classified as mild irritants (category 2B) according to the UN GHS. 相似文献
103.
Background
The rice PLASTOCHRON (PLA) genes PLA1 and PLA2 regulate leaf maturation and the temporal pattern of leaf initiation. Although the function of PLA genes in the leaf initiation process has been analyzed, little is known about how they affect leaf growth. Previously, we suggested that PLA1 and PLA2 function downstream of the gibberellin (GA) signal transduction pathway. In the present study, we examined the phenotype of a double mutant of pla and slender rice 1 (slr1), which is a constitutive GA response mutant. By analyzing these double mutants, we discuss the relationship between PLA-related and GA-dependent pathways and the possible function of PLA genes in leaf growth.Findings
Single slr1 and pla mutants exhibited elongated and dwarf phenotypes in the vegetative stage, respectively. The stature and leaf size of the pla1/slr1 and pla2/slr1 double mutants were intermediate between those of the pla and slr1 single mutants. However, the effects of slr1 on leaf elongation were markedly suppressed in the pla1 and pla2 mutant backgrounds. On the other hand, the change in cell length in the double mutants was almost the same as that in the single mutants. An expression analysis of genes involved in GA biosynthesis and catabolism indicated that feedback regulation functioned normally in the pla/slr1 double mutants.Conclusions
Our genetic results confirm that PLA genes regulate leaf growth downstream of the GA pathway. Our findings also suggest that PLA1 and PLA2 are partly required for GA-dependent leaf elongation, mainly by affecting cellular proliferation. 相似文献104.
Summary Two potato (Solanum tuberosum L., cv. Irish Cobbler) Kunitz-type proteinase inhibitors (PKPI) were previously described to be present in the soluble fraction
of proteins from tubers in the early stages of development. One of them became insoluble in mature tubers, being extractable
from this material in presence of urea. Amino acid sequencing showed that the soluble and insoluble PKPI were identical to
each other. Also, immunolocalization using the protein A-gold method showed that both proteins were present inside the vacuole
in free (intravacuolar space) and aggregated forms. The density of PKPI in the vacuolar protein aggregates increased from
developing to mature tubers. showing that the soluble-insoluble state of this protein is related to the aggregation levels.
Purified PKPI precipitated in vitro. mainly in presence of high calcium concentrations and low pH, but this precipitated form
was not as stable as aggregates found in vivo. Based on the results obtained, a model of PKPI insolubilization in vivo is
discussed. 相似文献
105.
Kazuhito Itoh Daisuke Goto Kaoru Sueyasu Kousuke Suyama Hiroki Yamamoto 《Soil Science and Plant Nutrition》2013,59(3):333-339
Different microbial communities characterized by the Biolog pattern were developed in the rhizosphere of radish grown on a rockwool hydroponic system treated with chloropicrinfumigated and non-fumigated soil suspensions although no differences were observed in their viable counts. Different microbial communities also were developed in the rhizosphere and non-rhizosphere. After the development of microbial communities in the rhizosphere, bud cells of Fusarium oxysporum that causes vascular wilt of radish plants were inoculated, and disease symptoms were examined. Treatment with the non-fumigated soil suspension was much more effective than that with the fumigated one in controlling the disease, indicating that the Biolog method might be applicable to characterize microbial communities that control the disease caused by F. oxysporum. 相似文献
106.
Sumio Itoh 《Soil Science and Plant Nutrition》2013,59(4):653-656
Abstract A field experiment was conducted to investigate the effect of N nutrition under water stress conditions on the cell membrane stability (CMS) measured by the polyethylene glycol (PEG) test, plant water relations, and osmotic adjustment in maize (Zea mays L.). Stressed plants showed greater adaptations to water deficits at higher N levels. Leaf water potential and osmotic potential decreased, turgor potential increased, and CMS increased with increasing N levels. Osmoregulation was evident at higher N levels with sugar and K as the major osmotic contributors. Results suggest that osmoregulation is influenced by N nutrition. 相似文献
107.
Serovars of Erysipelothrix species isolated from the tonsils of healthy cattle in Japan. 总被引:2,自引:0,他引:2
Serovars of 79 Erysipelothrix isolates recovered from the tonsils of healthy slaughtered cattle over a 1-year period in Japan were determined by an agar double-diffusion precipitation system using typing sera representing all the known serovars, 1 through 23 and type N, of Erysipelothrix. A total of 43 out of the 79 Erysipelothrix isolates could be classified into nine serovars but the remaining 36 isolates were untypable. Of 42 isolates identified as Erysipelothrix rhusiopathiae, 4, 6, 2, 3, 1,12, 13 and 1 isolates belonged to serovars 1b, 2, 5, 9, 12, 13, 19 and 21, respectively. One isolate belonged to Erysipelothrix tonsillarum serovar 3. 相似文献
108.
109.
Tomomi TANAKA Satoshi OHKURA Yoshihiro WAKABAYASHI Takenobu KUROIWA Kiyosuke NAGAI Natsumi ENDO Akira TANAKA Hisanori MATSUI Masami KUSAKA Hiroaki OKAMURA 《The Journal of reproduction and development》2013,59(6):563-568
The aim of the present study was to determine if the estradiol-induced luteinizing
hormone (LH) surge is influenced by the constant exposure to TAK-683, an
investigational metastin/kisspeptin analog, that had been established to depress the
pulsatile gonadotropin-releasing hormone (GnRH) and LH secretion in goats.
Ovariectomized goats subcutaneously received TAK-683 (TAK-683 group, n=6) or vehicle
(control group, n=6) constantly via subcutaneous implantation of an osmotic pump.
Five days after the start of the treatment, estradiol was infused intravenously in
both groups to evaluate the effects on the LH surge. Blood samples were collected at
6-min intervals for 4 h prior to the initiation of either the TAK-683 treatment or
the estradiol infusion, to determine the profiles of pulsatile LH secretion. They
were also collected at 2-h intervals from –4 h to 32 h after the start of estradiol
infusion for analysis of LH surges. The frequency and mean concentrations of LH
pulses in the TAK-683 group were remarkably suppressed 5 days after the start of
TAK-683 treatment compared with those of the control group (P<0.05). On the other
hand, a clear LH surge was observed in all animals of both groups. There were no
significant differences in the LH concentrations for surge peak and the peak time of
the LH surge between the TAK-683 and control groups. These findings suggest that the
effects of continuous exposure to kisspeptin or its analog on the mechanism(s) that
regulates the pulsatile and surge mode secretion of GnRH/LH are different in
goats. 相似文献
110.
Yasuyuki Tsukamasa Keisuke Kato Bimol Chandra Roy Yasunori Ishibashi Toru Kobayashi Tomohiro Itoh Masashi Ando 《Fisheries Science》2013,79(2):349-355
The color of the red muscle of pelagic fish is used as an indicator of freshness, and sustaining a bright red color is important for maintaining the commercial value of pelagic fish. Feeding fish with antioxidant compounds can delay metmyoglobin (metMb) formation, but the process requires long-term feeding. Live cultured Japanese horse mackerel Trachurus japonicus were used in this study. After anesthetization, 2 ml of blood was drawn from blood vessels, and a parenteral solution including an antioxidant compound was injected. Browning, metMb formation, and lipid oxidation of dark muscle during chilled storage were delayed by injecting sodium l-ascorbate as an antioxidant compound in the blood vessels of live fish. 相似文献