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131.
Yuta NISHIYAMA Yasuhiro FUKUYAMA Takuya MARUO Shinichiro YODA Masataka IWANO Shinpei KAWARAI Hideki KAYANUMA Kensuke ORITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1206
Tegafur is a prodrug of fluoropyrimidine 5-fluorouracil (5-FU), while TS-1TM is an oral fixed-dose combination of three active drugs, tegafur, gimeracil, and oteracil. This pilot study evaluated the safety of tegafur/gimeracil/oteracil in the treatment of cancers in dogs. Tegafur/gimeracil/oteracil was administered orally at a mean dose of 1.1 mg/kg twice daily on alternate days, Monday-Wednesday-Friday, every week to 11 dogs with tumors. Partial response and stable disease were observed in one dog each, whereas six exhibited progressive disease. Three dogs were not assessed. Adverse events, the most serious being grade 2, were noted in seven dogs. Adverse events were acceptable, and the drug was effective in some dogs. Therefore, tegafur/gimeracil/oteracil may be useful for treating malignant solid tumors in canines. 相似文献
132.
Purification and characterization of trypsin from the spleen of tongol tuna (Thunnus tonggol) 总被引:1,自引:0,他引:1
Klomklao S Benjakul S Visessanguan W Kishimura H Simpson BK 《Journal of agricultural and food chemistry》2006,54(15):5617-5622
Trypsin from tongol tuna (Thunnus tonggol) spleen was purified to 402-fold by ammonium sulfate precipitation, followed by a series of chromatographic separations. The molecular mass of trypsin was estimated to be 24 kDa by size-exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Trypsin appearing as a single band on native PAGE showed the maximal activity at pH 8.5 and 65 degrees C. It was stable in a wide pH range of 6-11 but unstable at the temperatures greater than 50 degrees C. The enzyme required calcium ion for thermal stability. The activity was strongly inhibited by 1.0 g/L soybean trypsin inhibitor and 5 mM TLCK and partially inhibited by 2 mM ethylenediaminetetraacetic acid. Activity was lowered with an increasing NaCl concentration (0-30%). The enzyme had a Km for Nalpha-p-tosyl-L-arginine methyl ester hydrochloride of 0.25 mM and a Kcat of 200 s-1. The N-terminal amino acid sequence of trypsin was determined as IVGGYECQAHSQPHQVSLNA and was very homologous to other trypsins. 相似文献
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Sheauchi Cheng Yukio Hiwatashi Hideki Imai Mitsuru Naito Tatsuka Numata 《Journal of Forest Research》1998,3(4):199-204
The major causes of deforestation and degradation of natural resources in the Belete-Gera Forest of Ethiopia are coffee production
activities and encroachment into forestland to expand farmland and pasture. Population growth, and the government’s land-reform
and re-settlement programs have caused local residents to lose harmony with the land. Forest management in this area hasn’t
yet been fully developed. The objectives of this study are to identify the extent of deforestation and natural resource degradation,
in preparation for a sound management plan. Encroachment of farmland and pasture into natural forest during the past four
years has been identified through interviews and aerial photo interpretation. The encroachment rate is 1.45% per year. Encroachment
occurred mostly on areas with gentle slopes adjacent to populated villages and along roads and footpaths. The extent and impact
of coffee production activities were examined through agency documents, forest survey data and vegetation survey. It is estimated
that up to 49% of the accessible natural forest is under the influence of coffee production activities, among which collecting
of naturally grown coffee beans has the least and the coffee plantations has the most impact on the natural forest. Coffee
plantations in natural forest have reduced the forest density and species diversity. Age structure of the trees is limited
to mature and old classes only, which eventually endangers their function as shade for coffee plantations.
This project was funded by the Japan International Cooperation Agency. 相似文献
137.
Gaku Kanno Takahito Yamaguchi Hideki Kishimura Etsurou Yamaha Hiroki Saeki 《Fish physiology and biochemistry》2010,36(3):637-645
Trypsin from the pyloric ceca of masu salmon (Oncorhynchus masou) cultured in fresh water was purified by a series of chromatographies including Sephacryl S-200, Sephadex G-50 and diethylaminoethyl
cellulose to obtain a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) and native PAGE.
The molecular mass of the purified trypsin was estimated to be approximately 24,000 Da by SDS–PAGE. The enzyme activity was
strongly inhibited by phenylmethylsulfonyl fluoride, soybean trypsin inhibitor, and N
α
-p-tosyl-l-lysine chloromethyl ketone. Masu salmon trypsin was stabilized by calcium ion. The optimum pH of the masu salmon trypsin
was around pH 8.5, and the trypsin was unstable below pH 5.0. The optimum temperature of the masu salmon trypsin was around
60°C, and the trypsin was stable below 50°C, like temperate-zone and tropical-zone fish trypsins. The N-terminal 20 amino acid sequence of the masu salmon trypsin was IVGGYECKAYSQPHQVSLNS, and its charged amino acid content was
lower than those of trypsins from frigid-zone fish and similar to those of trypsins from temperate-zone and tropical-zone
fish. In the phylogenetic tree, the masu salmon trypsin was classified into the group of the temperate-zone fish trypsin. 相似文献
138.
Hideki ENDO Truong Son NGUYEN Dinh Duy NGUYEN Motoki SASAKI Junpei KIMURA Tatsuo OSHIDA Masaya YAGO Ngoc Tu LY Thi Tham NGUYEN Masaharu MOTOKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1534
Morphological variation of the skull was examined in the northern treeshrew (Tupaia belangeri) from various localities across Southeast Asia. Through a multivariate analysis, the treeshrews from South Vietnam exhibited distinct morphological characteristics compared to other populations from Thailand and Laos, and Malaysia. The plots of the specimens of North Vietnam are not randomly mixed with Thailand plots segregation in the scatteregrams of canonical discriminant analysis. Since the skulls of the population from North Vietnam were morphologically similar to those form central Laos and northern and northeastern Thailand, the zoogeographical barrier effect of Mekong River was not clearly confirmed. The population of the Kanchanaburi in western Thailand is clearly smaller in size compared to the other populations. The southern border of the distribution of this species is determined by the Isthmus of Kra or Kangar-Pattani Line. In the northern treeshrew, which is distributed from southern China to Bangladesh and southern Thailand, we have detected osteometrical geographical variation driven by geography. These results indicate that the skull morphology in the Tupaia glis-belangeri complex distinctively differs in South Vietnam, western Thailand, and southern Thailand. The zoogeographical barrier and factor separating these districts are expected to clarify in the future. 相似文献
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140.
Tadahide Kurokawa Takuma Okamoto Koichiro Gen Susumu Uji Koji Murashita Tatsuya Unuma Kazuharu Nomura Hajime Matsubara Shin‐Kwon Kim Hiromi Ohta Hideki Tanaka 《Journal of the World Aquaculture Society》2008,39(6):726-735
The occurrence of morphological deformities under different rearing water temperatures (18, 20, 22, 24, 26, 28, and 30 C) was examined in Japanese eel larvae. The rates of hatching and survival until yolk resorption at 22–26 C were higher than those at other water temperatures. Fertilized eggs never hatched at 18 and 30 C. The rates of occurrence of abnormal larvae reared at the water temperatures 24–28 C were lower than those at 20 or 22 C. Pericardial edema and lower jaw deformities occurred most frequently at lower temperatures (20 and 22 C). In contrast, the incubation temperature did not significantly affect the relative frequency of some neurocranial deformities and of spinal curvature. These results imply that the optimal temperatures for rearing Japanese eel eggs and embryos are 24–26 C from the viewpoints of survival and deformity. 相似文献