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351.
Takumi Fujino Hideki Sawada Hiromichi Mitamura Reiji Masuda Nobuaki Arai Yoh Yamashita 《Fisheries Science》2017,83(3):367-372
Populations of sea cucumbers, including the Japanese common sea cucumber Apostichopus japonicus, have been seriously depleted worldwide due to overfishing. Mark–recapture study is an efficient means of collecting ecological data. However, the use of such a method in sea cucumbers is difficult because they lack hard tissues in the body wall. Here we tested the viability of various tagging methods on A. japonicus. First, we applied conventional tags using four different methods [single spaghetti (T-bar) tagging, double spaghetti tagging, ribbon tagging, and Atkins tagging] to ten individuals per method in aquaria for 14 days. Of the methods used, single spaghetti tagging had the highest retention rate. Then we examined the retention rate of single spaghetti tags on ten individual sea cucumbers for up to approximately 6 months in rearing conditions. The single spaghetti tagging method showed a retention rate of 100% over at least 7 days, and 50% of the tags remained embedded after 56 days. The longest duration of tag retention was 174 days, at which time the experiment was terminated. These results indicate that single spaghetti tagging is reliable for both short- and longer-term studies, making it a useful tool for ecological and conservation studies in sea cucumbers. 相似文献
352.
Thi Hien BUI Khanh Thuan NGUYEN Shunsuke IKEUCHI Haruka YANAGAWA Yomogi SATO Thi Hong To TRAN Minato OKUMURA Takeshi NIWA Takahide TANIGUCHI Hideki HAYASHIDANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1790
From 2012 to 2021, prevalence of pathogenic Yersinia in wild rodents captured in Fukushima Prefecture, Japan was investigated twice a year to clarify the ecology of this pathogen in wild rodent populations. Pathogenic Yersinia enterocolitica O8 was isolated from 13 (1.7%) of 755 wild rodents. The Y. enterocolitica O8 isolates harbored three virulent genes (ail, fyuA, and virF). This pathogen was isolated repeatedly from wild rodents in April 2015, 2016, and 2017, in June and November 2020, and in April 2021, which was 6 of 19 times of observations. All Y. enterocolitica O8 isolates showed the same PFGE patterns. These results indicated that the same clone of pathogenic Y. enterocolitica O8 has been maintained in wild rodent populations in Fukushima Prefecture. Therefore, wild rodent populations contribute substantially to the continuous transmission of Y. enterocolitica O8 and its persistence in the ecosystem. This is the first report on the isolation of pathogenic Y. enterocolitica O8 in wild rodents in Fukushima Prefecture, Japan. 相似文献
353.
Yukiho Sasaoka Taichi Takagi Shunta Michiba Yohei Yamamoto Yuya Kumagai Hideki Kishimura 《Marine drugs》2021,19(10)
In a previous study, we found that the collagen peptides prepared from the by-products of Bester sturgeon had an inhibitory effect on elevated blood glucose levels in a glucose tolerance test with ICR mice. In the present study, we examine the mechanism of the effect of sturgeon collagen peptides (SCPs) in detail. When glucose was orally administered to mice along with the SCPs, it was found that the glucose remained in the stomach for a longer time. In the above tests, the amount of glucose excreted in the feces of mice also increased. On the contrary, it was revealed that the SCPs have a dipeptidyl-peptidase-IV (DPP-IV) inhibitory ability in an in vitro test. In subsequent oral and intravenous glucose administration tests, glucagon-like peptide-1 (GLP-1) and insulin levels in the blood of mice were maintained at high levels. These results suggested the following three mechanisms: SCPs slow the rate of transportation of glucose from the stomach into the small intestine, resulting in delayed glucose absorption; SCPs suppress the absorption of glucose in the small intestine and excrete it from the body; SCPs inhibit DPP-IV in the blood and maintain a high GLP-1 level in blood, which in turn stimulates insulin secretion. 相似文献
354.
Shigeru Katayama Toshihiro Nishio Zensuke Iseya Hideki Kishimura Hiroki Saeki 《Fisheries Science》2009,75(2):491-497
A highly viscous polysaccharide solution was extracted from Gagome Kjellmaniella crassifolia in 20°C water. The eluted sugar concentration was 0.16%, and the extracted carbohydrates consisted of fucoidan, laminaran,
and alginate at an approximately ratio of 8.2:0.8:1.0. An increase in the extraction temperature resulted in a less viscous
solution, even though the amount of eluted sugar was higher than that obtained at a lower temperature. The most viscous extraction
solution was obtained at a neutral pH, with a more acidic or alkaline extraction solution being a less viscous. When the
highly viscous polysaccharide solution was heated, the viscosity decreased markedly with increasing temperature. The viscosity
of the polysaccharide solution increased after dialysis against water and decreased with the addition of either KCl or NaCl.
However, the viscosity was recovered to previous levels by following re-dialysis against water. The removal of divalent cations
by EDTA and the re-addition using CaCl2 or MgCl2 also caused reversible changes to the viscosity. These characterizations will be useful for widespread applications of viscous
K. crassifolia polysaccharides. 相似文献