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91.
Yasuko Kifuji Hideaki Hanzawa Yuuichi Terasawa Ashutosh Takeshi Nishio 《Euphytica》2013,190(2):289-295
One hundred sixty-one EST-SNP markers were newly developed for analysis of QTLs for resistance to black rot caused by Xanthomonas campestris pv. campestris by determining EST sequences of a resistant line obtained from cabbage ‘Early Fuji’ and a susceptible broccoli line. A linkage map consisting of nine linkage groups was constructed with a total of 209 markers, including these new SNP markers and previously reported DNA markers. F2 plants grown in a field for 1 month were inoculated by spraying bacteria of race 1, and disease severity of each plant was recorded. Three QTLs, i.e., QTL-1, QTL-2, and QTL-3, were detected on linkage group C2, C4 and C5, respectively. QTL-1, which showed the highest LOD score and additive effect, was again detected in another F2 population used the next year, suggesting QTL-1 to be a major QTL. QTL-2 and QTL-3 could be minor QTLs influenced by environmental factors. The genomic region harboring QTL-1 showed synteny with a region from 5.3 to 7.4 Mb from the short arm end of chromosome 5 of Arabidopsis thaliana, which is rich in TIR-NBS-LRR family genes. The identified SNP markers in QTL-1 are considered to be useful in marker-assisted selection for black rot resistance in Brassica oleracea lines. 相似文献
92.
93.
Takase Mai Yoneyama Yohei Murata Masataka Hibi Kyoko Ren Huifeng Endo Hideaki 《Fisheries Science》2012,78(3):691-698
We developed a wireless mediator-type biosensor system to rapidly monitor blood glucose concentrations in free-swimming fish.
Glucose oxidase was used to determine the glucose concentration, but fluctuations in the oxygen concentrations in the samples
affected the measurements. In this work, we used a mediator, a synthetic electron acceptor, as a substitute in the oxidation–reduction
reaction, thus ensuring that changes in the concentration of oxygen do not affect the measurements of glucose concentration
performed by the sensor. We especially focused on the use of ferrocene as a mediator. The resulting enzyme sensor utilized
a Pt–Ir wire (diameter 0.178 mm) as the working electrode and Ag/AgCl paste as the reference electrode. Chitosan was used
to fix ferrocene and glucose oxidase to the working electrode. The characteristics of the sensor were tested, and it was found
to be capable of measuring glucose independent of the oxygen concentration of the sample. Its reproducibility was also tested,
and the results showed that the sensor was suitable for performing measurements in vivo. The sensor was then inserted into
to eyeball interstitial sclera fluid in order to wirelessly monitor the glucose concentration in free-swimming fish. Measurements
were taken for a total of 48 h. During these measurements, artificial stress was applied to the fish. The glucose concentrations
of fish rise with their stress levels. The output current from the sensor gradually increased during the application of stress,
which hinted that the stress was monitored by this system. 相似文献
94.
Kentaro Hayashi Kentaro Takagi Izumi Noguchi Karibu Fukuzawa Hiroyuki Takahashi Tatsuya Fukazawa Hideaki Shibata Yasumi Fujinuma 《Water, air, and soil pollution》2009,200(1-4):33-46
The present study aimed to elucidate the atmosphere–forest exchange of ammoniacal nitrogen (NHX-N) at a young larch ecosystem. NHX-N exchanges were measured at a remote site in northernmost Japan where 4-year-old larches were growing after a pristine forest had been clear-cut and subsequent dense dwarf bamboo (Sasa) had been strip-cut. The site was a clean area for atmospheric ammonia with mean concentrations of 0.38 and 0.11 μg N m?3 in snowless and snow seasons, respectively. However, there was a general net emission of NHX-N. The annual estimated emission of NHX-N of 4.8 kg N ha?1 year?1 exceeded the annual wet deposition of 2.4 kg N ha?1 year?1, but the weekly exchange fluxes may have been underestimated by 28–60%. The main cause of the ammonia loss from the young larch ecosystem was probably enhanced nitrogen supply stimulated by the cutting of the pristine forest and Sasa, in particular, the Sasa. 相似文献
95.
Kazuhiro Toyoda Eriko Kawakami Hideaki Nagai Taiki Shiobara-Komatsu Kaori Tanaka Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2014,80(3):222-229
Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance. 相似文献
96.
Hiromi Ikeura Hideaki Takahashi Fumiyuki Kobayashi Michio Sato 《Journal of plant nutrition》2017,40(1):115-127
To provide the necessary oxygen for plant growth, the effectiveness of different generating methods of microbubbles (MB), a gas-water circulation type or a pressurization type (pressurization treatment), was investigated by comparing the growth characteristics and morphological observation of Brassica campestris L. var. Komatsuna Matsum. The plant growth in the pressurization treatment were significantly less than those in the circulation treatment at 3 weeks after planting. In plants grown in the pressurization treatment, epidermal cell abrasion and plasmolysis, indicators of cell death, were observed in root tip cells at 3 weeks after planting. The growth inhibition of plant in the pressurization treatment is suggested to be related to the oxidization of Fe in the nutrient solution under super-saturated dissolved oxygen conditions, the generation of hydroxyl radicals from MB, the oxidization of the root tip cell membrane, and osmotic stress to the roots. 相似文献
97.
98.
Izuta H Shimazawa M Tazawa S Araki Y Mishima S Hara H 《Journal of agricultural and food chemistry》2008,56(19):8944-8953
Endoplasmic reticulum (ER) stress has been implicated in the pathogenesis of neurodegenerative and ischemic disorders. The purpose of this study was to evaluate the effects of Chinese propolis and its constituents [chrysin, galangin, pinocembrin, caffeic acid, and caffeic acid phenethyl ester (CAPE)] against tunicamycin-induced neuronal cell death in SH-SY5Y cells. Both Chinese propolis and chrysin concentration-dependently inhibited such cell death, the tunicamycin-induced activation of caspase-3, and the effects of tunicamycin on mitochondria [release of cytochrome c into the cytosol and disruption of the mitochondrial membrane potential (DeltaPsim)]. Furthermore, Chinese propolis and chrysin each inhibited staurosporine-induced cell death. These findings indicate that the inhibitory effects of Chinese propolis against neuronal cell death induced by ER stress or staurosporine may be exerted primarily by chrysin. Moreover, the mechanism underlying the protective effects may, at least partly, involve inhibitions of caspase-3 activity and the mitochondrial apoptotic pathway. 相似文献
99.
Fast‐to‐slow shift of muscle fiber‐type composition by dietary apple polyphenols in rats: Impact of the low‐dose supplementation 下载免费PDF全文
Wataru Mizunoya Shinpei Okamoto Hideo Miyahara Mariko Akahoshi Takahiro Suzuki Mai‐Khoi Q. Do Hideaki Ohtsubo Yusuke Komiya Mulan Qahar Toshiaki Waga Koichi Nakazato Yoshihide Ikeuchi Judy E. Anderson Ryuichi Tatsumi 《Animal Science Journal》2017,88(3):489-499
Our previous studies demonstrated that an 8‐week intake of 5% (w/w) apple polyphenol (APP) in the diet improves muscle endurance of young‐adult rats. In order to identify a lower limit of the dietary contribution of APP to the effect, the experiments were designed for lower‐dose supplementation (8‐week feeding of 0.5% APP in AIN‐93G diet) to 12‐week‐old male Sprague‐Dawley rats. Results clearly showed that the 0.5% APP diet significantly up‐regulates slower myosin‐heavy‐chain (MyHC) isoform ratios (IIx and IIa relative to total MyHC) and myoglobin expression in lower hind‐limb muscles examined (P < 0.05). There was a trend to increased fatigue resistance detected from measurements of relative isometric plantar‐flexion force torque generated by a stimulus train delivered to the tibial nerve (F(98, 1372) = 1.246, P = 0.0574). Importantly, there was no significant difference in the animal body‐phenotypes or locomotor activity shown as total moving distance in light and dark periods. Therefore, the present study encourages the notion that even low APP‐intake may increase the proportions of fatigue‐resistant myofibers, and has promise as a strategy for modifying performance in human sports and improving function in age‐related muscle atrophy. 相似文献
100.
Md. Majibur Rahman Khan Yasuo Gotoh Hideaki Morikawa Mikihiko Miura 《Fibers and Polymers》2006,7(4):333-338
Silk fibroin (SF) fiber from theAntheraea pernyi silkworm was treated with a 1.23 N iodine-potassium iodide (I2-KI) aqueous solution, and the structure and physical properties were investigated to clarify the effects of the iodine treatment. The noticeably high weight gain value of SF fiber, about 25 wt% was attributed to the absorption of polyiodide ions in the form of I3 ? and I5 ?. Fourier transform infrared spectroscopy and X-ray diffraction measurements suggested that polyiodide ions mainly entered the amorphous region. In addition, a new sharp reflection on the meridional direction, corresponding to a period of 7.0 Å, was observed and indicated the possibility of the formation of mesophase structure ofβ-conformation chains. Dynamic viscoelastic measurements showed that the damping tanδ peak at 270°C gradually shifted to lower temperature in the iodinated SF fibers, suggesting an enhancement of the molecular motion of the fibroin chains induced by the presence of polyiodide ions. With heating above 254 °C, the iodine component introduced intermolecular cross-linking of SF, and the melt flow of the sample was inhibited. The thermal decomposition stability of fibroin molecules was greatly enhanced by iodine treatment. 相似文献