Complement of integrated fertilizer management and integrated pest management concepts to ameliorate faba bean growth and yield

Twenty-one microbial preparations recommended for controlling pathogenic fungal strains causing root rot and wilt diseases of faba bean were investigated for antibiosis against several symbiotic and associative diazotrophs adopting a modified agar-plate-inhibition-zone assay. Rhizobium and Bradyrhizobium exhibited a somewhat similar susceptibility to biocontrol agents while associative diazotrophs showed variable responses. Azotobacter, compared to others, was severely inhibited by such bio-candidates. The members of the biofertilizer formulation ‘Biofertan’ did bear mixed cultivation with the majority of biocontrol agents. Among those, Bacillus subtilis was deemed the pioneer. In pot experiments, almost all the antagonists significantly restricted the severity of root rot and wilt diseases besides modifying faba bean seedling stand and improving plant development. This was very obvious with shoot biomass increases of >?50%. Moreover, the bioagents successfully recovered the legume establishment, seriously injured due to pathogenic fungal infection. Simultaneous inoculation with Rhizobium and biocontrol agents provided more growth stimulation compared to either when introduced individually. Field-grown faba beans were inoculated with the diazotroph and representatives of biocontrol strains by two different methods, seed coating and over-head soil. Growth parameters determined were the highest when the legume plant was seed-coated by Rhizobium simultaneously over-head soil inoculated with the bacterial bioagents; this was reported with Bacillus subtilis and Pseudomonas aerugenosa. The significance of combined application of biofertilizer and bioagent to ensure cheap, clean and safe farm products is discussed.     

The effect of antimicrobial treatment and preventive strategies on bovine respiratory disease and genetic relatedness and antimicrobial resistance of Mycoplasma bovis isolates in a western Canadian feedlot

Feedlot calves (n = 3784) were systematically randomized and allocated in a 2 × 2 factorial study to receive metaphylactic oxytetracycline (OTC) on arrival or no antimicrobial, as well as florfenicol once subcutaneously or twice intramuscularly (48 h apart) if diagnosed with bovine respiratory disease (BRD). Calves of different treatment groups were comingled and followed from placement to re-implantation (~100 days). Animals receiving OTC had a reduced risk of BRD, an increased risk of arthritis, and no significant differences in average daily gain, BRD relapse, overall mortality, or BRD mortality. There were no significant differences between treatment protocols. Deep nasal swabs (n = 233) taken at arrival (n = 122), treatment (n = 77), and swabs from lungs and joints at postmortem (n = 34) were cultured for Mycoplasma bovis from 61 animals ill or dying of chronic pneumonia and arthritis and from 61 healthy calves. There was significant variation in diversity among isolates (n = 51) between study years and different cattle. Metaphylaxis or antimicrobial treatment did not affect the diversity of isolates. Except for tilmicosin, isolates were largely susceptible to tested antimicrobials.     

RADIOTHERAPY OF FELINE NASAL TUMORS A Retrospective Study of Nine Cases

Between 1978 and 1986, nine cats with nasal tumors were treated with radiation therapy. Rhinotomy was performed in six cats and diagnostic biopsy procedures were performed in three cats. For four of the specimens, a histologic review was significantly different from the initial diagnosis. At the time of analysis, one cat was alive and disease free 26.3 months after the first radiation therapy treatment; two of the cats were dead as a result of local recurrence, four were dead because of unrelated causes, and two were dead of unknown causes. The mean and median survival of cats in this study were 27.9 and 20.8 months, respectively, following the first radiation therapy. There was a 66.7% one-year, 44% two-year, and 33% three-year survival rate. Complications of the surgery and radiation therapy were minimal. In conclusion, the histologic evaluation of nasal neoplasms in cats is not straightforward and may require specialized histologic technics for accurate diagnosis. Radiation appears to be safe and may be efficacious in local control of feline nasal tumors.