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排序方式: 共有665条查询结果,搜索用时 15 毫秒
71.
Deposits of amyloid beta protein in the central nervous system of transgenic mice. 总被引:10,自引:0,他引:10
D O Wirak R Bayney T V Ramabhadran R P Fracasso J T Hart P E Hauer P Hsiau S K Pekar G A Scangos B D Trapp 《Science (New York, N.Y.)》1991,253(5017):323-325
Alzheimer's disease is characterized by widespread deposition of amyloid in the central nervous system. The 4-kilodalton amyloid beta protein is derived from a larger amyloid precursor protein and forms amyloid deposits in the brain by an unknown pathological mechanism. Except for aged nonhuman primates, there is no animal model for Alzheimer's disease. Transgenic mice expressing amyloid beta protein in the brain could provide such a model. To investigate this possibility, the 4-kilodalton human amyloid beta protein was expressed under the control of the promoter of the human amyloid precursor protein in two lines of transgenic mice. Amyloid beta protein accumulated in the dendrites of some but not all hippocampal neurons in 1-year-old transgenic mice. Aggregates of the amyloid beta protein formed amyloid-like fibrils that are similar in appearance to those in the brains of patients with Alzheimer's disease. 相似文献
72.
The (87)Sr/(86)Sr ratios in some bulk abyssal and alpine peridotites are too high to be binary mixtures of depleted mantle and seawater components. The apparent excess, or "orphan," (87)Sr appears to be separated from its radioactive parent. Such observations were widely held to be analytical artifacts. Study of several occurrences of orphan (87)Sr shows that the orphan component in abyssal peridotite is located in the alteration products of olivine and enstatite in the peridotite. The orphan (87)Sr is most likely introduced by infiltration of low-temperature (<200 degrees C) seawater bearing suspended detrital particulates. These particulates include grains of detrital clay that are partly derived from continental (that is, granitic) sources and thus are highly radiogenic. Orphan (87)Sr and other radiogenic isotopes may provide a tracer for low-temperature seawater penetrating into the oceanic crust. 相似文献
73.
T L Doering J Raper L U Buxbaum S P Adams J I Gordon G W Hart P T Englund 《Science (New York, N.Y.)》1991,252(5014):1851-1854
Trypanosoma brucei, the protozoan parasite responsible for African sleeping sickness, evades the host immune response through the process of antigenic variation. The variant antigen, known as the variant surface glycoprotein (VSG), is anchored to the cell surface by a glycosyl phosphatidylinositol (GPI) structure that contains myristate (n-tetradecanoate) as its only fatty acid component. The utilization of heteroatom-containing analogs of myristate was studied both in a cell-free system and in vivo. Results indicated that the specificity of fatty acid incorporation depends on chain length rather than on hydrophobicity. One analog, 10-(propoxy)decanoic acid, was highly toxic to trypanosomes in culture although it is nontoxic to mammalian cells. 相似文献
74.
Parsons BN Williams NJ Pinchbeck GL Christley RM Hart CA Gaskell RM Dawson S 《Veterinary journal (London, England : 1997)》2011,190(2):249-254
Dog ownership is considered a risk factor for campylobacteriosis in humans. This study investigated the prevalence and shedding of Campylobacter spp. in kennelled dogs. Faecal samples (n=399) were collected in longitudinal studies from 52 dogs in two kennels. Campylobacter spp. were isolated using charcoal-based selective agars and direct PCR. The prevalence of Campylobacter spp. in dogs in boarding kennels ranged from 46% (95% CI 22, 72) on entry, to 50% (95% CI 30, 70) overall, and in dogs in 'rescue' kennels from 68% (95% CI 49, 84) on entry to 73% (95%, CI 56, 87) overall. C. upsaliensis was isolated from 62% (95% CI 48, 73) of the dogs, whilst C. jejuni was isolated from 15% (95% CI 7, 26) of animals. The majority of infected dogs entered the kennels already carrying Campylobacter spp., and remained infected throughout their stay. However, in some cases, shedding appeared to commence after kennelling. Given that the prevalence of C. upsaliensis and C. jejuni was relatively high in dogs from both boarding and rescue kennels, such animals may pose a zoonotic risk. 相似文献
75.
Brown P Garner C Glass R Ridgway C Hart A 《Journal of agricultural and food chemistry》2004,52(12):3693-3701
Methods to refine the assessment of exposure of wild birds and mammals to pesticides required measurement of pesticide residues in very small samples of their diets. Sample sizes were in the 1-100 mg range, and the target residue for measurement was 0.01 mg/kg. Gas chromatography-mass spectrometry (GC-MS) with large volume injection was compared with the use of an accelerator mass spectrometer (AMS) to measure residues of pesticide labeled at near-background levels with carbon-14. The GC-MS method was able to detect residues down to 0.1 ng per item of diet, and the AMS detected the radiolabel down to 1 mBq (0.06 disintegration per minute, 1 ng of pesticide at the specific activity used) per sample. The target residue level was achieved by the GC-MS method for samples down to 10 mg. The GC method appeared to be best suited to monitoring residues in field studies, and the AMS shows great potential for use in laboratory experiments concerning pesticide degradation. 相似文献
76.
D L Park B M Miller L P Hart G Yang J McVey S W Page J Pestka L H Brown 《Journal of the Association of Official Analytical Chemists》1989,72(2):326-332
A joint AOAC/IUPAC (International Union of Pure and Applied Chemistry) interlaboratory study of an enzyme-linked immunosorbent screening assay (ELISA) for aflatoxins was conducted in laboratories in Canada, France, Japan, South Africa, Switzerland, The Netherlands, Tunisia, and the United States. Twenty-eight samples of raw and roasted peanuts, corn, whole cottonseed, cottonseed meal, ammoniated cottonseed meal, and poultry feed containing various quantities of natural aflatoxins and supplemented when appropriate with aflatoxin B1 were distributed to participating laboratories for testing. The assay is based on conjugation of pure aflatoxin B1 to an enzyme and the competition between this conjugate and (free) aflatoxins in the product for aflatoxin-specific antibodies coated onto microtiter well walls. After a wash step to remove all unbound aflatoxins, a substrate, added to each well, is catalyzed from a colorless to a green solution by any bound enzyme-conjugated aflatoxin B1 present. The intensity of the color decreases as the amount of free aflatoxin B1 in the product increases. Overall correlation was good between ELISA and thin-layer chromatographic (TLC) results for cottonseed products and mixed feed. Variable results were reported for corn and peanut product samples. Although some positive samples (greater than 15 ng/g) of cottonseed products and mixed feed were reported to contain less than 15 ng/g by visual determination, a review of data for absorbance measurements showed that the contamination level was close to the greater than or equal to 15 ng/g standard and would not have been reported as negative under routine screening.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
77.
K. M. Hart St. R. McDow W. Giger D. Steiner H. Burtscher 《Water, air, and soil pollution》1993,68(1-2):75-90
Aerosol photoemission (APE) has been found to be an excellent surrogate measure of particulate PAH concentration in combustion aerosols under a variety of laboratory and field conditions. Samples of oil stove, automobile exhaust, and urban air particulate matter were concurrently analyzed for aerosol photoemission intensity and particulate PAH concentration. In-situ, real-time analysis of the particulate matter was performed by UV-induced electron photoemission. Two photon energies, 4.9 and 6.7 eV, were chosen for irradiation. The latter energy was more sensitive and less selective. Particulate PAH concentrations were determined by collecting the particulate matter on a filter, solvent extracting the filter, and analyses of the extracts by gas chromatography / mass spectroscopy. Linear correlations (0.84≤r2≤1.00) were observed between aerosol photoemission intensities and the sum of particulate PAH concentrations for laboratory and field studies under a wide variety of conditions. The studies performed to date cover a dynamic range of three orders of magnitude (1 to 1000 ng / m3). The time resolution is in the order of a few seconds. 相似文献
78.
B L Hart 《Journal of the American Veterinary Medical Association》2001,219(1):51-56
OBJECTIVE: To determine whether gonadectomy predisposes dogs to development of age-related behavioral changes linked to cognitive impairment. DESIGN: Cohort study. ANIMALS: 29 sexually intact male dogs, 63 spayed female dogs, and 47 castrated male dogs 11 to 14 years old. PROCEDURE: Information on possible impairments in 4 behavioral categories linked to cognitive impairment (orientation in the home and yard, social interactions, house training, and sleep-wake cycle) was obtained from owners of the dogs by use of a structured telephone interview format. A second interview was performed 12 to 18 months after the initial interview, and differences in responses were evaluated. RESULTS: Sexually intact male dogs were significantly less likely than neutered dogs to progress from mild impairment (i.e., impairment in 1 category) to severe impairment (i.e., impairment in > or = 2 categories) during the time between the first and second interviews. This difference was not attributable to differences in ages of the dogs, duration of follow-up, or the owners' perceptions of the dogs' overall health. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that the presence of circulating testosterone in aging sexually intact male dogs may slow the progression of cognitive impairment, at least among dogs that already have signs of mild impairment. Estrogens would be expected to have a similar protective role in sexually intact female dogs; unfortunately, too few sexually intact female dogs were available for inclusion in the study to test this hypothesis. There may be a need to evaluate possible methods for counteracting the effects of loss of sex hormones in gonadectomized dogs. 相似文献
79.
FREDRIK E. HOPPE DVM PHD DAVE A. HAGER DVM PAUL W. POULOS JR. DVM PHD STINA EKMAN DVM P. G. LINDGREN MD PHD 《Veterinary radiology & ultrasound》1986,27(4):99-101
A manual, ultrasound-guided biopsy technique was compared to a new automatic method using a new biopsy device. The liver and the left kidney of 10 dogs were biopsied using the two methods. The specimens from the biopsies were compared for size and quality. Results demonstrate that larger and higher quality samples were obtained with the new automatic method. 相似文献
80.