盘锦市环境空气污染现状及对策

通过对盘锦市环境空气质量的调查,阐述了该市空气污染现状,并提出了环境空气污染的防治对策及建议。     

宁夏贺兰山土壤金龟子群落特征研究

采用诱捕法调查了宁夏贺兰山6个不同生境中的土壤金龟子分布,并以群落相似性系数、欧氏距离和因子分析等方法对不同土壤金龟子群落之间的相似性进行了分析。共采集金龟子昆虫3475只,经鉴定有32种,分别隶属8科22属,其中有植食性种类4科12属19种,主要为鳃金龟科、花金龟和丽金龟科;粪食腐食性种类4科10属13种,主要为金龟科、蜉金龟科、粪金龟科的种类。对几种群落因子分析结果将6个土壤金龟子群落分为4类：一类为针叶林地和针阔混交林地,一类为典型草地和草甸草地,荒漠草地和灌丛草地各为一类。     

Location and transmission of QTL for multiple traits in the pedigree of soybean cultivars

In silico mapping for single trait was extended to analyze many agronomic traits in the pedigree of soybean. 26 agronomic traits were measured and 477 polymorphic markers chosen on public genetic map were genotyped on 14 inbreeding lines in the pedigree of Suinong14. We firstly determined 6 principal components from 26 agronomic traits using the principal component analysis and then constructed 6 “super traits” by the multiplication of the vector of the standardized original traits by the eigenvectors corresponding to the principle components. With in silico mapping, a total of 24 markers distributing on 13 linkage groups were detected separately as QTL responsible for 6 “super traits” and of which 14 QTL performed pleiotrpy. Tracing the transmission of functional genes in the pedigree, it was found that some genes were capable to explain the genetic mechanism for the contribution of exotic germplasms and domestic founders to soybean cultivars in the improvement of the performance and quality.     

Energy saving effect analysis of water system renovation in a coking plant

A coking plant extended many times, and its capacity of the cooling water system is not match with it, the consumption of new water is much more than that used by others in the same industry. The water system renovation is addressed for an extended coking plant. The installation of two 600 m3/h cooling towers and restoration the existing two cooling towers are to promote the cooling capacity so that the amount of discharge and feed could be reduced. At the same time, the pipe net for waste water reutilisation is installed, by which part of the waste water is reused as supplement water for quenching hot coke and the other was piped to ironworks for reuse. With the field test, the water consumption per ton of coke production is cut down from 9.03 m3 to 3.21 m3. And the annual water saving is more than 6×106 m3. Furthermore, the electric consumption is reduced by 2×106 kWh and about 3×106 yuan of annual running cost is saved.     

温室环境参数智能控制系统的设计

依据温室控制要求,介绍了模糊控制技术在温室控制系统中的应用,给出了模糊控制器的实现过程。实践表明,该控制器具有良好的控制效果。     

Monitoring the emission of volatile organic compounds from the leaves of Calocedrus macrolepis var. formosana using solid-phase micro-extraction

In this study, solid-phase micro-extraction (SPME) fibers coated with polydimethylsiloxane/divinylbenzene (PDMS/DVB), coupled with gas chromatography/mass spectrometry, were used to monitor the emission patterns of biogenic volatile organic compounds (BVOCs) from leaves of Calocedrus macrolepis var. formosana Florin. in situ. In both sunny and rainy weather, the circadian profile for BVOCs from C. macrolepis var. formosana leaves has three maximum emission cycles each day. This kind of emission pattern might result from the plant’s circadian clock, which determines the rhythm of terpenoid emission. Furthermore, emission results from the leaves demonstrated that the circadian profile of α-pinene observed was opposite to the profiles of limonene and myrcene, a difference that may be attributable to two different subpathways for terpenoid biosynthesis.     

Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines

Three canine mammary tumor (CMT) cell lines, namely DE-E, DE-F and DE-SF, have been established from a surgically excised specimen of a malignant mammary tumor. These CMT cell lines have been cultured for over 200 passages. The cell doubling time was estimated to be approximately 30 h for all three cell lines. DE-E, DE-F and DE-SF were epithelial, fibroblast and spindle fibroblast in morphology, respectively. Under electron microscope, DE-F and DE-SF cells displayed a higher nucleus/cytoplasm ratio as compared with DE-E. Variation in chromosome number was also observed in the three cell lines. In addition to the morphological characteristics, these cell lines displayed differential patterns of several known mammary tumor cell markers. Following xenotransplantation of the CMT cells into nude mice, DE-F and DE-SF developed tumors within 2 weeks, whereas DE-E failed to develop any visible tumor up to 8 weeks after injection. Lastly, the CMT cell lines exhibited differential chemoresistance to several anti-tumor drugs, including melatonin, cyclosporine A, tamoxifen and indole, suggesting that these cell lines can be used as a comparative experimental model for the tumorigenesis of mammary carcinomas and a valuable tool for anti-cancer drug screening.     

The phylogeographic system survey of native sheep breeds in the eastern and southern Central Asia

The genetic diversity and phylogenetic survey of native sheep breeds in the eastern and southern Central Asia were assessed in the present study. The clustering, principal components, structure and F statistics all demonstrate that the native sheep breeds in these regions be classified into two genetic groups: Mongolia‐Tibetan sheep group and South‐Southeast Asia sheep group. The Mongolia sheep group and the Tibetan sheep group had a certain degree of gene communication from the ancient times. In the present study we demonstrated that the Chinese native sheep populations belonged to Mongolia‐Tibetan sheep group. However, the relationships among the sheep populations in Mongolia sheep group in China were not closely related to the geographical distance among sheep populations.     

Dog erythrocyte antigens 1.1, 1.2, 3, 4, 7, and Dal blood typing and cross‐matching by gel column technique

Background: Testing for canine blood types other than dog erythrocyte antigen 1.1 (DEA 1.1) is controversial and complicated by reagent availability and methodology. Objectives: The objectives of this study were to use available gel column technology to develop an extended blood‐typing method using polyclonal reagents for DEA 1.1, 1.2, 3, 4, 7, and Dal and to assess the use of gel columns for cross‐matching. Methods: Dogs (43–75) were typed for DEA 1.1, 1.2, 3, 4, 7, and Dal. Methods included tube agglutination (Tube) using polyclonal reagents, a commercially available DEA 1.1 gel column test kit (Standard‐Gel) using monoclonal reagent, and multiple gel columns (Extended‐Gel) using polyclonal reagents. Blood from 10 recipient and 15 donor dogs was typed as described above and cross‐matched using the gel column technique. Results: Of 43 dogs typed for DEA 1.1, 23, 25, and 20 dogs were positive using Standard‐Gel, Extended‐Gel, and Tube, respectively. Typing for DEA 1.2 was not achievable with Extended‐Gel. For 75 dogs typed for DEA 3, 4, and 7, concordance of Extended‐Gel with Tube was 94.7%, 100%, and 84%, respectively. Dal, determined only by Extended‐Gel, was positive for all dogs. Post‐transfusion major cross‐matches were incompatible in 10 of 14 pairings, but none were associated with demonstrable blood type incompatibilities. Conclusions: Gel column methodology can be adapted for use with polyclonal reagents for detecting DEA 1.1, 3, 4, 7, and Dal. Agglutination reactions are similar between Extended‐Gel and Tube, but are more easily interpreted with Extended‐Gel. When using gel columns for cross‐matching, incompatible blood cross‐matches can be detected following sensitization by transfusion, although in this study incompatibilities associated with any tested DEA or Dal antigens were not found.