Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase

Mobilization of fatty acids from triglyceride stores in adipose tissue requires lipolytic enzymes. Dysfunctional lipolysis affects energy homeostasis and may contribute to the pathogenesis of obesity and insulin resistance. Until now, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. Here, we report that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. It is interesting that ATGL contains a "patatin domain" common to plant acyl-hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.     

Venus: an upper limit on intrinsic magnetic dipole moment based on absence of a radiation belt

On the basis of the absence of energetic electrons (E(e) 45 kiloelectron volts) and protons (E(p) 320 kiloelectron volts) associated with Venus to within a radial distance of 10,150 kilometers from the center of the planet and using a physical similitude argument and the observational and theoretical knowledge of the magnetosphere of Earth, we conclude that the intrinsic magnetic dipole moment of Venus is almost certainly less than 0.01 and probably less than 0.001 of that of Earth. Corresponding upper limits on the magnetic field at the equatorial surface of Venus are about 350 and 35 x 10(-5) gauss, respectively.     

Separation of metallic from semiconducting single-walled carbon nanotubes

We have developed a method to separate metallic from semiconducting single-walled carbon nanotubes from suspension using alternating current dielectrophoresis. Our method takes advantage of the difference of the relative dielectric constants of the two species with respect to the solvent, resulting in an opposite movement of metallic and semiconducting tubes along the electric field gradient. Metallic tubes are attracted toward a microelectrode array, leaving semiconducting tubes in the solvent. Proof of the effectiveness of separation is given by a comparative Raman spectroscopy study on the dielectrophoretically deposited tubes and on a reference sample.     

The effect of systemic pesticides on total glycoalkaloid content of potato tubers at harvest

Two studies were conducted to determine the effect of systemic pesticides on the potato tuber total glycoalkaloid (TGA) content at harvest. In a field study, a pretuberization application of three systemic insecticides (disulfoton, aldicarb, carbofuran) and three systemic fungicides (thiabendazole, benomyl, thiophanate methyl) on three potato cultivars (Cobbler, Katahdin and B5141-6) showed carbofuran to be the only treatment significantly different (P= .05) from the control. The tuber TGA content of the carbofuran treatment was lower than that of the control. In a greenhouse study, carbofuran applied during tuberization to three potato varieties (Norland, Kennebec, Abnaki) produced a highly significant increase (P= .01) in the tuber TGA content. A significant varietal difference (P=.01) in response to the carbofuran treatment was also evidenced.     

Russette: A Russet-skin potato variety,combining high quality,high yields,and adaptation to the Northeastern states and Florida

Russette is a new, late maturing, russet potato variety adapted primarily for the northeastern United States and northern Florida. Tubers are oval to oblong, smooth with a medium-russet skin, and shallow eyes. Its specific gravity is similar to those of BelRus and Russet Burbank in areas of adaptation. Glycoalkaloid content is about 11 mg/100 g fresh weight. Baking quality is excellent, flesh is white, and best color of french fries is obtained from tubers stored at or above 13°C (55°F). Its long rest period makes it amenable to storage at a relatively high temperature before a chemical sprout inhibitor is needed. Russette is immune to virus A and leaf roll-induced tuber net necrosis; highly resistant to Verticillium wilt, tuber pinkeye, and tuber heat necrosis; and has good tolerance to scab, early blight, and Rhizoctonia infection of sprouts, stolon, and roots. Losses to rots and shrinkage in storage have been minimal. Russette is not resistant to viruses X, S, Y, and leaf roll and bacterial ring rot. Russette emerges to a stand about 10 days later than most varieties, however, subsequent vine growth is rapid until tuberization begins. Large-sized tubers may develop hollow heart under a late-season regime of high nitrogen and high moisture. A 22–30 cm (9″- to -12″) seed-piece spacing and 220 kg/ha (180 pounds/a) of nitrogen are recommended for production on mineral soils.     

The use ofStreptococcus zymogenes for estimating tryptophan and methionine bioavailability in 17 foods

As part of a cooperative study assessing amino acid bioavailability and/or protein quality, the provisional method of Boyneet al. (Brit J Nutr 21: 181–206) was used to assay 17 protein sources for methionine and tryptophan availability withS. zymogenes. Pronase was used as the predigesting enzyme. Product composition was found to affect reproducibility. The microbial assay results correlated positively with results from rat growth studies on the same foods (p=0.05), and were generally accurate in identifying products of lower protein quality. Defatting four high-fat products increased microbial values in the methionine assay, but only the chicken franks and the sausage values in the tryptophan assay. Heating non-fat milk increased methionine values slightly. Low values for rolled oats were further reduced by finer grinding.     

The potential for incorporating herbicides into a mulch farming system in Costa Rica

Experiments on the use of herbicides in the mulch farming system of the North Atlantic Zone of Costa Rica have given promising results. In order to ascertain whether extension of investigations to farm-field trials is warranted, 20 farms were surveyed, providing data which facilitated comparison between the net benefits of the traditional weed-control methods and those of the new system incorporating herbicides. The results indicate that, without taking crop-yield increase into account, approximately 30% of the farms sampled would have increased their cash income by adopting the new weed-control system. This figure increases to 50% when the yield increase recently reported is considered. The new system is particularly promising in areas heavily infested with weeds such as Rottboellia exaltata and Panicum maximum. In our view, these results justify advancing the research from experimental plots to farm-field trials.     

Uptake of Metronidazole in Artemia at Different Developmental Life Stages

Abstract

The purpose of this study was to examine the capacity of live brine shrimp Artemia spp. to accumulate metronidazole at different developmental life stages. Metronidazole is used in fish as an antiparasitic medication. An effective drug delivery method is to enrich the Artemia with metronidazole and offer them as live feed to the infected fish, usually ornamental species and other small fishes. Artemia cysts were hatched and then soaked in a metronidazole solution (0.05%) at instars 1–3 of larval development. Our findings indicated that Artemia were able to accumulate metronidazole at levels considered therapeutic to other animals and humans (25–100 mg/kg). However, the levels varied depending on the stage of larval development. Artemia accumulated the highest levels of metronidazole (137–143 mg/kg) when they started filter feeding (instar 2), whereas newly hatched Artemia (instar 1) contained the lowest level (85 mg/kg). Based on this study and a review of the literature, a new protocol recommended for enriching Artemia with metronidazole consists of soaking the Artemia in a 0.05% metronidazole solution for 3 h at room temperature. Because metronidazole is relatively insoluble in water, it must first be dissolved in warm water with continuous stirring.

Received December 5, 2010; accepted January 30, 2011