Fabrication of Biocompatible PLGA/PCL/PANI Nanofibrous Scaffolds with Electrical Excitability

Application of electrospun nanofibrous scaffolds has received immense attention in tissue engineering. Fabrication of scaffolds with appropriate electrical properties plays a key role in neural tissue engineering. Since fibers orientation in the scaffolds affects the growth and proliferation of the cells, this study aimed to prepare aligned electrospun conductive nanofibers by mixing 1 %, 10 % and 18 % (w/v) doped polyaniline (PANI) with polycaprolactone (PCL)/poly lactic-coglycolic acid (PLGA) (25/75) solution through the electrospinning process. The fibers diameter, hydrophilicity and conductivity were measured. In addition, the shape and proliferation of the nerve cells seeded on fibers were evaluated by MTT cytotoxicity assay and scanning electron microscopy. The results revealed that the conductive nanofibrous scaffolds were appropriate substrates for the attachment and proliferation of nerve cells. The electrical stimulation enhanced neurite outgrowth compared to those PLGA/PCL/PANI scaffolds that were not subjected to electrical stimulation. As polyaniline ratio increases, electric stimulation through nanofibrous PLGA/PCL/PANI scaffolds results in cell proliferation enhancement. However, a raise more than 10 % in polyaniline will result in cell toxicity. It was concluded that conductive scaffolds with appropriate ratio of PANI along with electrical stimulation have potential applications in treatment of spinal cord injuries.     

Effects of melatonin on oocyte maturation in PCOS mouse model

The purpose of oocyte in vitro maturation is generation of mature oocytes that could support future development. Efforts have been made to enhance oocyte developmental competence by developing optimal culture conditions. The present study is conducted to determine melatonin effects on quality of polycystic ovarian syndrome (PCOS) oocytes when it has been added during in vitro maturation, and immature oocytes were cultured in defined conditioned medium with and without different melatonin concentrations. Melatonin could significantly improve nuclear maturation of PCOS oocytes (81.1% vs. 56.3%, P < 0.05 were achieved with 10^?6 mol/L concentration). Cleavage rate was significantly higher in 10^?5 mol/L concentration compared to untreated oocytes in PCOS (54% vs. 35%, respectively) and it was significantly higher with 10^?6 mol/L concentration in the control group, 55% versus 38%, compared to untreated oocytes. This study showed that melatonin has the potential to induce oocyte nuclear maturation and guarantee fertilization potential. © 2016 Japanese Society of Animal Science     

Effects of neonatal diarrhea and other conditions on subsequent productive and reproductive performance of heifer calves

Diarrhea is one of the most frequent diseases of neonatal calves in dairy herds. The aim of this study was to evaluate the effects of neonatal diarrhea and other conditions on subsequent first lactation milk production and reproductive performance of heifer calves up to the first calving. Seven hundred heifer calves (350 with and 350 without the history of diarrhea in first month of life) were monitored from birth until a year after calving. For each heifer, birth season, birth weight, ease of birth and occurrence of diseases from birth to the end of first lactation were recorded as independent variables. Interval from birth to first service, interval from birth to conception, interval from birth to first calving and 305-days milk yield in the first lactation were recorded as dependent variables. Effects of explanatory variables on productive and reproductive indices were evaluated by survival analysis and general linear model. Heifers with the history of diarrhea during the first month of their lives showed lower daily hazard of conception [Hazard ratio: 0.85 (95%CI: 0.73–0.99)] and calving [Hazard ratio: 0.84 (95%CI: 0.72–0.98)] than those of healthy heifers. Subsequent reproductive performance of heifer calves was also associated with birth season and ease of birth. Further, heifer calves born with birth weight of below 35 kg produced an average of 518 and 506 l less milk in their first lactation compared to calves with a birth weight 40–45 (p = 0.013) and greater than 45 kg (P = 0.033), respectively. These findings showed that occurrence of neonatal diarrhea and other neonatal parameters are associated with harmful effects on subsequent production and reproductive performance.     

Estimation of the organic carbon content by the pattern recognition method

Studying the status of agricultural soils is one of the most important concerns in the agricultural sector. The soil organic carbon (SOC) is one of the main parameters and it plays an important role in improving soil properties. Hence, knowing this parameter is important in soil science. This study applied the pattern recognition (PR) method in predicting the SOC. Also, the ability of this method was compared with different methods such as the Radial Basis Function Network (RBF), Multilayer Perceptron Neural Network (MLP), Multiple Linear Regression (MLR) and Support Vector Regression (SVR). To compare the results, four performance criteria, namely, root mean square errors (RMSE), the Nash–Sutcliffe efficiency (NS), Willmott’s Index of agreement (WI), mean absolute error (MAE) and Taylor diagrams were used. Results indicated that the PR model performed significantly better than the MLP, MLR, SVR, and RBF models for the estimation of the SOC.     

Projected impacts of global warming on cropping calendar and water requirement of maize in a humid climate

In this research, the influence of climate change on maize cultivation was investigated and then, the possible solutions for adopting this natural hazard in the coasts of Caspian Sea in Iran was assessed. Weather data were generated for the 2011–2100 period using a statistical downscaling model under different climatic scenarios. Reference evapotranspiration (ETo) was calculated using a Neuro-fuzzy inference system. Cop water requirement was calculated by multiplying ETo by crop coefficients. Increased cardinal temperatures during 2011–2100 led to shifting in the planting date backward by 10–26 days. In addition, the projected global warming has a considerable effect on the duration of the vegetative growth stage resulting in earlier harvesting. However, the duration of the reproductive stage is less affected. Despite the obvious reduction in the length of the growing season, crop water requirement will increase by 10.6–15.3% in the future due to 1.64–28.4% increase in ETo. However, changing the cultivation time may lead to 11.2–264.5 m³ ha^?1 water saving during the whole cropping cycle through affecting both ETo and the crop growth cycle. This result demonstrates that management of the maize cropping calendar can be an effective way to achieve sustainable agriculture under future climate conditions in the study area.     

Correction to: The effect of a phytogenic feed additive (Digestrom P.E.P) on growth performance,proximate composition,hematological and immunological indices of juvenile beluga sturgeon Huso huso

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