全文获取类型
收费全文 | 13453篇 |
免费 | 940篇 |
国内免费 | 1311篇 |
专业分类
林业 | 950篇 |
农学 | 750篇 |
基础科学 | 614篇 |
1496篇 | |
综合类 | 6915篇 |
农作物 | 872篇 |
水产渔业 | 476篇 |
畜牧兽医 | 1921篇 |
园艺 | 1018篇 |
植物保护 | 692篇 |
出版年
2024年 | 96篇 |
2023年 | 304篇 |
2022年 | 664篇 |
2021年 | 703篇 |
2020年 | 582篇 |
2019年 | 541篇 |
2018年 | 441篇 |
2017年 | 724篇 |
2016年 | 412篇 |
2015年 | 621篇 |
2014年 | 699篇 |
2013年 | 855篇 |
2012年 | 1232篇 |
2011年 | 1169篇 |
2010年 | 1155篇 |
2009年 | 994篇 |
2008年 | 976篇 |
2007年 | 864篇 |
2006年 | 732篇 |
2005年 | 602篇 |
2004年 | 386篇 |
2003年 | 228篇 |
2002年 | 207篇 |
2001年 | 226篇 |
2000年 | 178篇 |
1999年 | 54篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 10篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1965年 | 1篇 |
1964年 | 1篇 |
1962年 | 2篇 |
1960年 | 1篇 |
1958年 | 1篇 |
1956年 | 11篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 359 毫秒
991.
YAN Jin-chuan WU Zong-gui HE Song-qing LING Ling HAN Wen-yu FAN Jie ZHANG Suo-long 《园艺学报》2001,17(10):998-1001
AIM:To investigate effects of OX-LDL and VitE on the levels of IL-6,IL-8 and TNF-α in human umbilical vein endothelial cells(HUVEC).METHODS: Human umbilical vein endothelial cells were obtained by in vitro culture. HUVEC treated with or without Vit E was incubated with OX-LDL, and the levels of IL-6, IL-8 and TNF-α were determined by enzyme-linked immunosorbent assy technique. RESULTS:50 μg/L,100 μg/L, 200 μg/L OX-LDL induced the release of IL-6,IL-8 and TNF-α by HUVEC in a dose-dependent manner. Compared with the control group , the levels of IL-6 and IL-8 were significantly increased at 6-12 h of stimulation with OX-LDL . Maximal levels of IL-6 and IL-8 occurred after 24-36 h, reaching a plateau maintained for at least 48 h. TNF-α rose after 2-6 h in HUVEC, and reached a maximum after 12 h. In contrast to IL-6 and IL-8, TNF-α declined after 48 h. However, when VitE (50 mg/L,100 mg/L,200 mg/L)was added, it can significant inhibited the release of IL-6, IL-8 and TNF-α in a dose-dependent manner, and after 48 h these cytokines have no diference between OX-LDL+VitE groups and OX-LDL groups. CONCLUSION: OX-LDL can obviously stimulate the production of IL-6,IL-8 and TNF-α in vascular endothelial cells, which can significantly be inhibited by VitE in a short time. 相似文献
992.
AIM: To study the effect of mild hypothermia on energy metabolism and hydroxyl radical production as well as delayed neuronal death (DND) in hippocampus during cerebral ischemia/reperfusion in gerbils.METHODS: Forebrain ischemia was induced by occluding bilateral common carotid arteries with aneurysm clamps for 10 min in gerbils. The DND in hippocampal CA1 sector was assessed by histological examination, and hydroxyl radical, ATP (adenosine triphosphate), ADP (adenosine diphosphate),AMP (adenosine monophosphate) levels were determined by high performance liquid chromatography with electrochemical or ultraviolet detection. RESULTS: The number of survival neuronal in hippocampal CA1 sector in mild hypothermia + I/R group was more than that in I/R group after ischemia/reperfusion 96 h. The content of 2,3-DHBA (2,3- dihydroxybenzoic acid) in hippocampus in I/R group was much higher than those in sham operation and mild hypothermia + I/R group after reperfusion 6 h (P<0.01), but there were no significant differences in 2,3-DHBA outputs among 3 groups 48 h and 96 h after reperfusion. There were no obvious differences in ATP, ADP, AMP level in hippocampus among 3 groups 6 h after reperfusion. However, the content of ATP,ADP,AMP in mild hypothermia + I/R group was much higher than those in I/R group 48 and 96 h after reperfusion (P<0.01).CONCLUSION: Mild hypothermia can reduced DND by improving the cerebral energy metabolism during forebrain ischemia/reperfusion in gerbils. 相似文献
993.
994.
PEI Yaping ZHAO Jin SUN Na SUN Panpan SUN Yaogui FAN Kuohai YIN Wei LI Hongquan 《畜牧兽医学报》1956,51(12):3068-3075
Ovarian granulosa cells provide a special microenvironment for follicle formation and maturation through interaction with oocytes and their own secretion. A variety of harmful stimuli can cause granulosa cell apoptosis and metabolic disorders, reduce the quality of oocytes and have a negative impact on embryo formation. Zearalenone (ZEA) is a common cause of ovarian granulosa cells injury in the livestock industry, which is produced by mycotoxins, and lack of effective treatment drug. Therefore, in the current study zearalenone was used to induce ovarian granulosa cell injury and to explore the protective effect of caffeic acid on zearalenone-induced ovarian granulosa cell apoptosis in mice. Mouse ovarian granulosa cells were isolated by mechanical method, and indirect immunofluorescence was used to identify the isolated cells. MTT assay was used to determine the effect of caffeic acid on the activity of normal mouse ovarian granulosa cells.After granulosa cells were co-treated with caffeic acid (200, 100 and 50 μg·mL-1) and ZEA for 24 hours, and control and ZEA group were set up at the same time, cell morphology and adherence were observed under a microscope. MTT was also used to detect cell viability. Caspase-3 mRNA expression level was detected by qRT-PCR. Cleaved-caspase-3 and cleaved-PARP protein expre-ssion levels were determined by Western blot. The results showed that positive FSHR staining appeared in cell cytoplasm of the test group, which confirmed that the isolated cells were mouse ovarian granulosa cells. The cell viability was above 90% which showed that caffeic acid had no toxic effect on granulosa cells. Compared with control group, ZEA group had smaller cell size, poor adherence, increased cell gap, and significant reduction in cell viability (P<0.001). Furthermore, the relative expression of caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein level were significantly increased (P<0.001) compared with the control group. After caffeic acid treatment, cell gap was reduced, adherence was tight, cell viability was significantly increased (P<0.001). Caffeic acid significantly reduced zearalenone-induced increase in caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein expression level (P<0.001). This study indicated that caffeic acid can restore granulosa cell viability by inhibiting ZEA-induced apoptosis. 相似文献
995.
CHENG Zengqing FAN Gencheng DOU Xiaolong LIU Hongxiang SHEN Maoxin XU Quangang YANG Hanchun 《畜牧兽医学报》1956,51(10):2463-2471
The aim of this study was to identify pathogens, which caused pericardial effusion, hepatomegaly and bleeding at a chicken farm in Henan province, and furthermore to analyze effectiveness of immunogenic proteins of the pathogen. This study employed virus isolation, serological assays, PCR and sequencing analysis, animal experimentation, E. coli expression and protein purification, immunogenicity and challenge test and other methods. The results showed that virus was isolated in 7-day-old SPF chicken embryonated eggs, inoculated via the yolk sac route by two blind passages. Viral confirmation was carried out using PCR techniques, and showed a 900-bp-long fragment which shared a 100% homology with the Hexon gene of the serotype C4 strain. Serum neutralization results indicated that this isolate avian adenovirus was the group I type 4 avian adenovirus, named HN-ZK strain. The virus could induce CPE on primary hepatocytes of chicken embryo, and its titer was 107.5 TCID50·0.1 mL-1. Animal experimentation illustrated that the isolated virus caused 100% (10/10) typical symptoms and pathogenicity in 35-day-old SPF chickens. Furthermore, the DNA of the isolate virus was used as a template to express Fiber-2 fragment, with a molecular weight of 33 kDa by using the E. coli expression system, and the protein was concentrated and purified to 300 mg·mL-1 after centrifugation and purification. SPF chickens were immunized with different doses of the purified Fiber-2 protein, and showed that a dose of 20 μg per chick was completely resistant to challenge of the virulent virus, suggesting the purified Fiber-2 protein has better immunogenicity. This study can provide data for diagnosing the hydropericardium hepatitis syndrome, and developing a genetic engineering subunit vaccine. 相似文献
996.
主要农艺性状的表型评价是青萝卜育种的重要依据。对48 份青萝卜种质资源的33 个主要农艺性状进行变异分析、相关性分析、主成分分析和聚类分析,结果表明:9 个数量性状均有不同程度的变异,单株质量变异系数最大,为32.46%,叶宽变异系数最小,为14.67%;以肉质根为主的典型农艺性状间存在复杂的相关性,相关性极显著的有29 对,相关性显著的有8 对;从33 个农艺性状提取出9 个主成分,累计贡献率为78.937%,基本反映了青萝卜地上部和地下部重要农艺性状信息;在平方欧式距离系数为8.5 时,可将48 份供试青萝卜种质资源分为3 个组群,第Ⅰ组群包括33 份材料,主要特征是株高、开展度、叶长、叶宽和叶片数在3 组中最小,第Ⅱ组群包括14 份材料,主要特征为株型、叶片大小中等,第Ⅲ组群只有1份材料,其株高、开展度、单株质量和单根质量在3 组中最大。 相似文献
997.
998.
The interaction between JEV and the host at the miRNAs level was preliminarily explored by studying the miRNAs expression profiles of primary neurons in mice infected with JEV. Total RNA of JEV-infected and uninfected primary neurons of the suckling mice was extracted individually by Trizol and then analyzed miRNA expression profiles by high-throughput sequencing analysis. Significantly differentially expressed miRNAs were selected for verification by real-time quantitative PCR. Through bioinformatics analysis, 26 miRNAs with significant expression differences were screened out, among which 18 miRNAs were up-regulated and 8 miRNAs were down-regulated. The results of quantitative real-time PCR of the JEV-E gene indicated that the expressions of mir-21a-3p mir-223-5p mir-147-3p mir-155-5p and mir-146a-5p could promote the expression of JEV-E gene in neurons, while the expression of mir-301a was just on the contrast. The expression of miRNAs in primary neurons could affect the replication of JEV. This study provided the theoretical basis and direction for further studies on the regulatory function of miRNAs in the mechanism of neural dysfunction induced by JEV. 相似文献
999.
1000.