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21.
Geelen SN Jansen WL Sloet van Oldruitenborgh-Oosterbaan MM Breukink HJ Beynen AC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(5):478-481
The aim of this study was to establish the dose-response relationship between fat intake and heparin-released plasma lipoprotein lipase (LPL) activity in horses. Eight mature trotters were fed 4 rations with different fat levels (3.0, 5.0, 7.7, or 10.8% fat in the dry matter) according to a 4 X 4 Latin square design. The experimental rations consisted of hay and different concentrates; the concentrates and hay were given in a 3:1 ratio on an energy basis. Soybean oil was added to the concentrates at the expense of isoenergetic amounts of glucose. Blood samples were taken at the end of each dietary period, which lasted 3 weeks. Fat feeding was found to increase heparin-released plasma LPL activity in a dose-dependent fashion. When the data from this study and previous studies were combined it was calculated that an increase in fat intake by 1 g/kg dry matter is associated with an increase in LPL activity by 0.98 micromol fatty acid released-mL(-1) x h(-1). Fat feeding raised the plasma concentrations of total cholesterol, high-density lipoprotein cholesterol, and phospholipids. Diet did not have a statistically significant effect on plasma triacylglycerol concentrations. The results are discussed in the light of the possible enhancing effect of fat feeding on the oxidative capacity of skeletal muscle. 相似文献
22.
E H Jansen R H van den Berg H van Blitterswijk R Both-Miedema R W Stephany 《The Veterinary quarterly》1984,6(1):5-7
Radioimmunochemical detection (RIA) following fractionation of urine extracts via high performance liquid chromatography (HPLC) turned out to be a very specific method for the identification of stilbene derivatives in bovine urine. Combination of the high resolution of the HPLC with a specific RIA is a suitable method to discriminate between the presence of different stilbene derivatives like diethylstilboestrol (DES), dienoestrol (DE) and hexoestrol (HEX) or other unknown compounds interfering in the celite-RIA used in the screening. Using this screening method 8200 samples of bovine urine were investigated on the presence of stilbene derivatives of which 133 were classified as 'positive'. In 106 'positive' urines the presence of DES was shown and in 19 'positive' urines the presence of DE or HEX, using the method described in this report whereas in 8 'positive' urines an unknown immunochemical active compound was detected. During 1.5 year of comparative investigation no qualitative discrepancies occurred between the results of the HPLC-immunogram procedure and the final confirmation by high resolution gas chromatography-mass spectometry (GCMS). 相似文献
23.
Serological Studies of Parasitized Cattle: II. Electrophoretic Properties of Serial Serum Samples 下载免费PDF全文
Electrophoretic examinations using cellulose acetate strips were made on 17 serum samples collected at two-week intervals from each of three calves before and for about four months after they were placed on parasite-infected pastures in June, 1964. Four additional serum samples taken during the winter months when the animals were stabled and two obtained after they were again exposed to infected pastures in June 1965, were also examined. Two control animals of the same age, that were placed on clean pasture in 1964 and not exposed to infected pasture until June 1965, were bled on the same dates and their sera examined in parallel. All five calves showed an increase in the proportion and amount of γ-globulin in their serum as they increased in age but the gain was greatest in the two exposed calves that developed the highest complement-fixing titres with nematode antigens. Complement-fixing titres rose slowly in the other exposed calf and its serum γ-globulin level remained relatively comparable to that of the two controls. 相似文献
24.
Complement Titres of Naturally and Artificially Raised Piglets II. Comparison with the Results of Electrophoretic Examinations 下载免费PDF全文
Delma Cochrane C. L'Ecuyer Christine E. Rice 《Canadian journal of veterinary research》1964,28(2):38-42
Serial serum samples from piglets in one naturally-raised and three artificially-raised litters, 19 animals, were examined by cellulose acetate electrophoresis. The electropherograms of all day-old suckling and colostrum deprived piglets showed five definite bands, the first, third, fourth and fifth corresponding in position to the albumin, alpha-, beta- and gamma-globulin regions for adult pig serum. The second, well-defined band was located between the albumin and main alpha-globulin bands. This component, considered to be alpha1-globulin or fetuin, was no longer visible as a distinct band in the electrophoretic pattern for sera of three-week old suckling piglets or of five-week old artificially-raised piglets. No consistent relationship was observed between the rate of increase in haemolytic complement titre and the proportional changes in the various categories of serum globulins. 相似文献
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26.
Plasma fentanyl concentrations in awake cats and cats undergoing anesthesia and ovariohysterectomy using transdermal administration 总被引:1,自引:0,他引:1
Objective To measure the plasma fentanyl concentrations achieved over time with transdermal fentanyl patches in awake cats and cats undergoing anesthesia and ovariohysterectomy. Study design Randomized prospective experimental study. Animals Twenty‐four purpose‐bred cats. Methods Cats were randomly assigned to three groups for Part I of a larger concurrent study. Group P received only a 25 μg hour?1 transdermal fentanyl patch. Group P/A received the patch and anesthesia. Group A received only anesthesia. After a minimum 1‐week washout period, the cats were randomly reassigned to two groups for Part II of the larger study. Group P/A/O received the patch, anesthesia and ovariohysterectomy. Group A/O received anesthesia and ovariohysterectomy. Patches were left in place for 72 hours and plasma samples were obtained for fentanyl analysis while the patches were in place, and for 8 hours after patch removal for cats in Group P, P/A, and P/A/O. Results The 25 μg hour?1 transdermal fentanyl patches were well tolerated by the cats in this study (mean body weight of 3.0 kg) and no overt adverse effects were noted. Mean plasma fentanyl concentrations over time, mean plasma fentanyl concentrations at specific times (8, 25, 49, and 73 hours after patch placement), time to first detectable plasma fentanyl concentration, time to reach maximum plasma fentanyl concentration, maximum plasma fentanyl concentration, mean plasma fentanyl concentration from 8 to 73 hours, elimination half‐life, and total area under concentration (AUC) were not statistically different among the groups. Conclusions Halothane anesthesia and anesthesia/ovariohysterectomy did not significantly alter the plasma fentanyl concentrations achieved or pharmacokinetic parameters measured, when compared with awake cats. There was a high degree of individual variability observed both within and between groups of cats in parameters measured. Clinical significance The high degree of variability observed suggests that careful observation of cats with fentanyl patches in place is required to assess efficacy and any potential adverse effects. Anesthesia and anesthesia/ovariohysterectomy do not appear to alter plasma fentanyl concentrations achieved by placement of a 25 μg hour?1 transdermal fentanyl patch when compared to cats not undergoing these procedures. 相似文献
27.
Nielsen C Olver CS Schutten MM Twedt DC 《Journal of the American Veterinary Medical Association》2003,223(11):1623-7, 1600
A 6-year-old castrated male Dalmatian was evaluated because of hematemesis. The dog had lived its entire life in South Dakota and Wyoming and had never traveled outside of these states. Results of laboratory testing were compatible with iatrogenic acute renal failure and gastrointestinal tract ulceration secondary to previous nonsteroidal anti-inflammatory drug and corticosteroid administration. Differential diagnoses for clinical signs and laboratory abnormalities that existed prior to these treatments included multisystemic infectious or inflammatory disease and neoplasia. Four-quadrant abdominocentesis did not yield any fluid, but because intra-abdominal disease was still suspected, diagnostic peritoneal lavage was performed. Fluid that was obtained was markedly cellular, and there were numerous extracellular structures with a round to oval shape; a 1-microm-thick, clear-staining capsule; a basophilic interior; and broad-based budding. Organisms were consistent with Blastomyces spp, and fungal culture yielded Blastomyces dermatitidis. Treatment with liposomal amphotericin B and itraconazole was recommended but could not be initiated because of the client's financial constraints. At necropsy, disseminated blastomycosis involving the stomach, small intestines, urinary bladder, omentum, mesentery of the small intestine, and abdominal wall musculature was seen. To our knowledge, peritoneal involvement has not been reported in dogs with blastomycosis, and gastrointestinal tract involvement has only rarely been reported. Findings in this dog suggest that diagnostic peritoneal lavage may be a useful technique in determining the cause of infectious peritonitis when the amount of abdominal fluid is below the limit of detection for abdominocentesis. 相似文献
28.
Kinzel S Hein S Stopinski T Koch J Buecker A Treusacher HP Schmachtenberg A Jansen T Eble M Küpper W 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(3-4):134-138
This study describes the experience with hypofractionated radiation therapy of squamous cell carcinoma and melanoma in dogs and cats. A total dose of 32-48 Gray (Gy) was delivered once a week in 8 Gy fractions. 34 animals in which a complete surgical excision was impossible were treated. There was no tumor detectable macroscopically in 14 patients at the beginning of radiation therapy. In 20 animals the median volume of the tumor was 9.9 cm3. The median survival times and the local tumor control of squamous cell carcinoma of the oral and nasal cavities and of the body are comparable to results which were reached with a Monday-Wednesday-Friday scheme. For the treatment of Melanoma the hypofractionated radiation therapy is first choice. There are no significant side effects. Late side effects did not occur. 88% of the owners are satisfied with this kind of treatment and would choose it again. 相似文献
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