Effects of supplemental feeding on gastrointestinal parasite infection in elk (Cervus elaphus): preliminary observations

The effects of management practices on the spread and impact of parasites and infectious diseases in wildlife and domestic animals are of increasing concern worldwide, particularly in cases where management of wild species can influence disease spill-over into domestic animals. In the Greater Yellowstone Ecosystem, USA, winter supplemental feeding of Rocky Mountain elk (Cervus elaphus) may enhance parasite and disease transmission by aggregating elk on feedgrounds. In this study, we tested the effect of supplemental feeding on gastrointestinal parasite infection in elk by comparing fecal egg/oocyst counts of fed and unfed elk. We collected fecal samples from fed and unfed elk at feedground and control sites from January to April 2006, and screened all samples for parasites. Six different parasite types were identified, and 48.7% of samples were infected with at least one parasite. Gastrointestinal (GI) nematodes (Nematoda: Strongylida), Trichuris spp., and coccidia were the most common parasites observed. For all three of these parasites, fecal egg/oocyst counts increased from January to April. Supplementally fed elk had significantly higher GI nematode egg counts than unfed elk in January and February, but significantly lower counts in April. These patterns suggest that supplemental feeding may both increase exposure and decrease susceptibility of elk to GI nematodes, resulting in differences in temporal patterns of egg shedding between fed and unfed elk.     

RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test,comparison with pot grown plants and validation

Background

In order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. This could be achieved through an optimization of both root architecture and root uptake ability and/or through the improvement of positive plant interactions with microorganisms in the rhizosphere. The development of devices suitable for high-throughput phenotyping of root structures remains a major bottleneck.

Results

Rhizotrons suitable for plant growth in controlled conditions and non-invasive image acquisition of plant shoot and root systems (RhizoTubes) are described. These RhizoTubes allow growing one to six plants simultaneously, having a maximum height of 1.1 m, up to 8 weeks, depending on plant species. Both shoot and root compartment can be imaged automatically and non-destructively throughout the experiment thanks to an imaging cabin (RhizoCab). RhizoCab contains robots and imaging equipment for obtaining high-resolution pictures of plant roots. Using this versatile experimental setup, we illustrate how some morphometric root traits can be determined for various species including model (Medicago truncatula), crops (Pisum sativum, Brassica napus, Vitis vinifera, Triticum aestivum) and weed (Vulpia myuros) species grown under non-limiting conditions or submitted to various abiotic and biotic constraints. The measurement of the root phenotypic traits using this system was compared to that obtained using “classic” growth conditions in pots.

Conclusions

This integrated system, to include 1200 Rhizotubes, will allow high-throughput phenotyping of plant shoots and roots under various abiotic and biotic environmental conditions. Our system allows an easy visualization or extraction of roots and measurement of root traits for high-throughput or kinetic analyses. The utility of this system for studying root system architecture will greatly facilitate the identification of genetic and environmental determinants of key root traits involved in crop responses to stresses, including interactions with soil microorganisms.

     

Changes in shell dimensions of pearl oysters, Pinctada mazatlanica (Hanley 1856) and Pteria sterna (Gould 1851), during growth as criteria for Mabé pearl implants

The relationship between the shell dimensions of pearl oysters. Pinctada mazatlanica (Hanley 1856) and Pteria sterna (Gould 1851). was studied to determine the possible change of form of the shell during growth. The intention was to determine the number, size and location of nuclei that could be implanted in oysters used for Mabé pearl production. Using the database of our Pearl Culture Research Programme developed in Bahía de La Paz, México, we obtained measurements of 500 shells of P. mazatlanica and 500 shells of P. sterna, representing 3 years of continuous growth under extensive culture conditions. The height-length, height-thickness and the height-weight relationships of both species were analysed, as was the height-wing length relationship for P. sterna. There was isometric growth up to 100 mm shell height, and negative-allometric growth after 120-125 mm for P. mazatlanica. The height-thickness relationship followed a linear model, whereas the height-weight relationship was exponential. No clear allometric pattern was found in P. sterna and all relationships fitted the power equation.     

Effects of no‐take area size and age of marine protected areas on fisheries yields: a meta‐analytical approach

Marine protected areas (MPAs) are often promoted as tools for biodiversity conservation as well as for fisheries management. Despite increasing evidence of their usefulness, questions remain regarding the optimal design of MPAs, in particular concerning their function as fisheries management tools, for which empirical studies are still lacking. Using 28 data sets from seven MPAs in Southern Europe, we developed a meta‐analytical approach to investigate the effects of protection on adjacent fisheries and asking how these effects are influenced by MPA size and age. Southern European MPAs showed clear effects on the surrounding fisheries, on the ‘catch per unit effort’ (CPUE) of target species, but especially on the CPUE of the marketable catch. These effects depended on the time of protection and on the size of the no‐take area. CPUE of both target species and the marketable catch increased gradually by 2–4% per year over a long time period (at least 30 years). The influence of the size of the no‐take area appeared to be more complex. The catch rates of the entire fishery in and around the MPA were higher when the no‐take areas were smaller. Conversely, catch rates of selected fisheries that were expected to benefit most from protection increased when the no‐take area was larger. Our results emphasize the importance of MPA size on its export functions and suggest that an adequate, often extended, time frame be used for the management and the evaluation of effectiveness of MPAs.     

A network meta‐analysis of threats to South American fish biodiversity

While environmental alterations have made Homo sapiens the hyperkeystone species of the globe, biotic homogenization initiated a new era, the “Homogenocene.” Still, some terrestrial and aquatic ecosystems in South America are considered pristine and wild, which can lead to a general faith that economic progress is consistent with conservation strategies, even without scientific support. We compiled anthropogenic threats to fish biodiversity in a hierarchical meta‐analysis, along with an evidence synthesis of threats related to biological invasions, based on peer‐reviewed research with the aim to represent the actual conservation status of the South American ichthyofauna. We highlighted human‐related threats and synergistic effects of biological invasions, climate change, environmental alterations (e.g., pollution, aquaculture and damming) and fisheries. Considering measures that reinforce novel alien fish (e.g., artificial hybrids or genetically modified) introductions, it became clear why an eventual increase in local or regional species richness is not always beneficial to aquaculture, biodiversity, human well‐being or nature. In fact, citizens in all societal roles, including scientists, should revise their concepts about threats to fish biodiversity. Environmental policies require more than taxonomic diagnostics to achieve conservation goals under an incompatible scenario of a multiplying number of fish species and biotic homogenization. We advocate for countries in South America using science‐based strategies useful to maintain their social and economic growth along with their “remaining nature.” We live a crucial moment when the government overlooks threats to biodiversity and uses agribusiness as the most acceptable manner of fuelling the economy.     

Persistent Contamination of Octopuses and Mussels with Lipophilic Shellfish Toxins during Spring Dinophysis Blooms in a Subtropical Estuary

This study investigates the occurrence of diarrhetic shellfish toxins (DSTs) and their producing phytoplankton species in southern Brazil, as well as the potential for toxin accumulation in co-occurring mussels (Perna perna) and octopuses (Octopus vulgaris). During the spring in 2012 and 2013, cells of Dinophysis acuminata complex were always present, sometimes at relatively high abundances (max. 1143 cells L⁻¹), likely the main source of okadaic acid (OA) in the plankton (max. 34 ng L⁻¹). Dinophysis caudata occurred at lower cell densities in 2013 when the lipophilic toxins pectenotoxin-2 (PTX-2) and PTX-2 seco acid were detected in plankton and mussel samples. Here, we report for the first time the accumulation of DSTs in octopuses, probably linked to the consumption of contaminated bivalves. Perna perna mussels were consistently contaminated with different DSTs (max. 42 µg kg⁻¹), and all octopuses analyzed (n = 5) accumulated OA in different organs/tissues: digestive glands (DGs) > arms > gills > kidneys > stomach + intestine. Additionally, similar concentrations of 7-O-palmytoyl OA and 7-O-palmytoly dinophysistoxin-1 (DTX-1) were frequently detected in the hepatopancreas of P. perna and DGs of O. vulgaris. Therefore, octopuses can be considered a potential vector of DSTs to both humans and top predators such as marine mammals.     

Detection of QTL for forage yield,lodging resistance and spring vigor traits in alfalfa (Medicago sativa L.)

Alfalfa (Medicago sativa L.) is an internationally significant forage crop. Forage yield, lodging resistance and spring vigor are important agronomic traits conditioned by quantitative genetic and environmental effects. The objective of this study was to identify quantitative trait loci (QTL) and molecular markers associated with increased forage yield, resistance to lodging, and spring vigor. A backcross population composed of 128 progeny was developed by crossing the breeding parents DW000577 (lodging susceptible) and NL002724 (lodging-resistant) and back-crossing an individual F₁ plant to the maternal parent (i.e. DW000577). A linkage map of NL002724 was developed based upon the segregation of 236 AFLP, SRAP, and SSR markers among the backcross progeny. The markers were distributed among 14 linkage groups, covering an estimated recombination distance of 1497.6 centiMorgans (cM). Replicated clones of both parents and backcross progeny were evaluated in the field for estimated forage yield, lodging, and spring vigor in Washington and Wisconsin during 2007 and 2008. Significant QTL were found for all three traits. In particular, two QTL for lodging resistance were identified that explained ≥14 % of trait variation, and were significant in all years and locations. Major QTL explaining over 25 % of trait variation for forage yield were detected in multiple environments at two separate locations on chromosome III. Several QTL for spring vigor were located in the same or similar positions as QTL for forage yield, possibly explaining the significant correlation between these traits. Molecular markers associated with the aforementioned QTL were also identified.     

Salinity tolerance and mycorrhizal responsiveness of native xeroriparian plants in semi-arid western USA

Restoration of salt-affected soils is a global concern. In the western United States, restoration of salinized land, particularly in river valleys, often involves control of Tamarix, an introduced species with high salinity tolerance. Revegetation of hydrologically disconnected floodplains and terraces after Tamarix removal is often difficult because of limited knowledge regarding the salinity tolerance of candidate native species for revegetation. Additionally, Tamarix appears to be non-mycorrhizal. Extended occupation of Tamarix may deplete arbuscular mycorrhizal fungi in the soil, further decreasing the success of revegetation efforts. To address these issues, we screened 42 species, races, or ecotypes native to southwestern U.S. for salinity tolerance and mycorrhizal responsiveness. As expected, the taxa tested showed a wide range of responses to salinity and mycorrhizal fungi. This variation also occurred between ecotypes or races of the same species, indicating that seed collected from high-salinity reference systems is likely better adapted to harsh conditions than seed originating from less saline environments. All species tested had a positive or neutral response to mycorrhizal inoculation. We found no clear evidence that mycorrhizae increased salinity tolerance, but some species were so dependent on mycorrhizal fungi that they grew poorly at all salinity levels in pasteurized soil.