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991.
992.
Embryo production in donkeys is inefficient compared with that in other livestock. Obtaining a sufficient number of MII oocytes is the first step to solving this problem. In this study, the number, morphology and maturation rates of cumulus-oocyte complexes (COCs) obtained from abattoir-derived ovaries or live jennies were compared. The diameter of follicles from abattoir-derived ovaries was measured and divided into group 1 (2–6 mm), group 2 (6–10 mm), group 3 (10–20 mm), group 4 (20–28 mm) and group 5 (>28 mm). The results showed that the number of follicles per ovary in group 2 (3.6 ± 0.28) and 3 (4.2 ± 0.90) was higher than that in the other groups (p < .05). The recovery rate in group 3 was higher than group 1 (48.8% vs. 26.8%, p = .00), but lower than group 5 (48.8% vs. 76.5%, p = .025). The percentage of grade A COCs in group 3 was higher than group 2 (59.3% vs. 39.5%, p = .00) and group 1 (59.3% vs. 26.7%, p = .00). Moreover, the percentage of grade A COCs in group 4 (55.0%, p = .710) and group 5 (46.2%, p = .351) was reduced compared with that in group 3. From the above results, the developing follicles (group ovum pick-up [OPU], 10–20 mm) and preovulation follicles (group OPU-Preov, >35 mm) were aspirated from live jennies using OPU. Although there was no difference in the recovery rates of COCs between group 3 and OPU (48.8% vs. 43.0%, p = .184), the percentage of grades A COCs in group OPU was higher than group 3 (72.5% vs. 59.3%, p = .036). There was no difference in the maturation rate between group 3 and OPU (60.3% vs. 69.3%, p = .171) after the COCs matured in vitro. The rates of recovery (72.2%) and maturation (92.3%) in group OPU-Preov were higher than those in other groups (p < .05). Moreover, the effects of maturation time and serum type on maturation rates were evaluated in groups B44 (44 h, FBS), B36 (36 h, FBS) and D44 (44 h, foetal donkey serum, FDS). These results indicated that the maturation rate in group B36 was lower than group B44 (13.1% vs. 47.0%, p = .00) and group D44 (13.1% vs. 53.3%, p = .00). In conclusion, the quality of donkey COCs from OPU was higher than that from abattoir-derived ovaries, the suitable time of donkey in vitro maturation (IVM) was 44 h, and FBS could be replaced with FDS in donkey IVM medium. 相似文献
993.
为了解北京市种猪场猪群伪狂犬病病毒(PRV)感染情况,采用酶联免疫吸附试验(ELISA),对2019—2021年3个区采集的10 850份血清样品进行PRV感染抗体(gE抗体)检测。结果显示:2019—2021年,北京市3个区种猪场群体表观阳性率分别为84.38%、77.78%、64.71%,群体真实阳性率分别为86.10%(95%CI:73.95%~98.24%)、79.37%(95%CI:60.39%~98.34%)、66.03%(95%CI:43.22%~88.83%),下降趋势不显著(P> 0.05);个体表观阳性率分别为53.95%、41.52%、35.59%,个体真实阳性率分别为55.05%(95%CI:53.05%~57.05%)、42.37%(95%CI:40.90%~43.84%)、36.32%(95%CI:34.85%~37.79%),呈显著下降趋势(P <0.05)。育成猪、经产母猪和后备母猪个体阳性率逐年下降,而育肥猪个体阳性率逐年升高,由2019年的38.45%,提高到2021年的60.79%。结果表明,北京市种猪场PRV感染得到一定的控制,但感染... 相似文献
994.
995.
Conventional mechanical method and mechanical method combined with vacuum freeze-drying technology were used to make the ultra-fine
powders of edible fungus (Auricularia auricular). The content of basic nutrients, amino acid, micro structure and their properties of raw edible fungus and the edible fungus
powders obtained with the two methods were analyzed and compared. The granularity size and micro-structure of the pulverized
samples were analyzed by SEM and TEM technology. The average granularity size of the edible fungus powder obtained with mechanical
method was 1–5 μm, while that obtained with mechanical method combined with vacuum freeze-drying process was 0.5–1 μm. The
ultra-fine powders of edible fungus obtained with the two methods had better water recovery capability and quality, and their
preserving time was longer than that of raw edible fungus. All the properties of the ultra-fine powders of edible fungus obtained
with the vacuum freeze-drying technology were evidently superior to that of the conventional mechanical method.
Biography: YANG Chun-yu (1975), female, Ph. D. in Engineering Technology Center of Forestry and Wood Workine, Machinery, Northeast
Forestry University, Harbin 150040, P. R. China.
Responsible editor: Zhu Hong 相似文献
996.
L. G. García-Montero J. L. Manjón S. Martín-Fernández G. Di Massimo 《Agroforestry Systems》2007,70(3):243-249
In Mediterranean pine forests, truffles and mushrooms generate greater profits than any other woodland products. However,
there are no studies on Tuber melanosporum Vittad. associated with pines. For this reason, we have carried out a study of this truffle in mountain woods with Pinus sylvestris L. and P. nigra Arnold subsp. salzmannii (Dunal) Franco, in central Spain. Two hundred and eight Tuber melanosporum burns were monitored for 7 years in five different habitats within the same geographical area. An ANOVA test confirmed significant
differences in carpophore production. In higher producing habitats, pines were less abundant. We also confirmed that in 433
burns, T. melanosporum was always unequivocally associated with the root base of Quercus or Corylus trees. Similarly, 14 truffle collectors confirmed that they had never found a single burn with carpophore production associated
exclusively with pines. Nevertheless, soil analyses indicated that the soil of these pine woods was very favourable to Tuber melanosporum. We therefore conclude that at present Pinus nigra
salzmannii and P. sylvestris are of little interest to Tuber melanosporum culture, as they hinder carpophore production. However, this study has also confirmed that Pinus nigra salzmannii and P. sylvestris mycorrhize easily with Tuber melanosporum, both in the laboratory and in natural environments. On this basis, we propose that pines may act as transmitters of T. melanosporum, although they do not induce fruiting. As a result, the commercial cultivation of Pinus nigra
salzmannii and P. sylvestris seedlings mycorrhized with Tuber melanosporum is not recommended in truffle culture at the present time. 相似文献
997.
以泡桐丛枝病类菌原体的敏感指示植物长春花苗为材料,用叶柄注射法注入感病泡桐叶汁液进行接种试验,结果表明,有1/4供试植株感病,其症状与泡桐丛枝病相一致,将感病植株叶脉切片镜检,发现有类菌原体,其形态、大小及结构,均与泡桐丛枝病类菌原体相同。为该病病原的传播及回接提供了一条简便的途径。 相似文献
998.
应用AHP构建城市森林树种综合评价指标体系 总被引:17,自引:0,他引:17
阐述了建立城市森林树种综合评价体系的指导思想与基本原则。建立了由7个抗性功能指标、5个生态功能指标5、个美学功能指标组成的城市森林树种综合评价指标体系;应用层次分析法(AHP),利用13位专家对评价指标成对比较的平均值构建了判断矩阵,进而确定了各层指标的权重值,并进行了一致性检验。研究表明,固碳释氧、抗污染、防风固沙、抗旱、滞尘5项指标的权重值最高,这与沈阳地区的基本状况以及城市森林树种选择的基本原则有着高度的一致性,研究在城市树种评价方面做了一次定性与定量、主观与客观相结合的有益尝试。 相似文献
999.
The effects of applying N(urea, 46% N), P(phosphate, 12% P2O5) and K(potassium sulphate, 54% K2O) fertilizers on the growth performance and biomass yield of 8-month-old Archidendron clypearia seedlings were examined by L9 orthogonal array with 3 replicates in the nursery of Zengcheng Forest Farm of Guangzhou, Guangdong Province. The test seedlings were potted with the subsoil of total N 0.0484%, total P 0.1518% and total K 1.2629%, and fertilized at the 2nd and 6th months with the application rates of 0, 10 and 20 mg of N, 0, 20 and 50 mg of P and 0, 10 and 20 mg of K per seedling. The results showed that the diameter at ground level(DGL), the height and the oven-dried weight biomass of 8-month-old seedlings differed significantly at P=0.05. The combined application dose of 20 mg N, 20 mg P and 0 mg K per seedling resulted in the best seedling growth and biomass yield. N fertilizer was a significant factor in the growth of DGL and height of A. clypearia seedlings. P fertilizer had no significant effects on both DGL and height, while K fertilizer only affected DGL significantly. Analysis of the leaf and root stoichiometry suggested that N may be the most single limiting nutrient, A. clypearia seedlings used N more efficiently than they used P. 相似文献
1000.
Costanza Di Stefano Vito Ferro Vincenzo Pampalone 《Land Degradation \u0026amp; Development》2016,27(2):239-247
Many process‐oriented erosion prediction models reproduce rill erosion as affected by site‐specific parameters, as for example, rill erodibility, and thus, their practical application requires the measurement of these parameters or their estimate. The aim of this paper was establishing a method for indirectly measuring field rill erodibility. A simple mathematical approach based on a known soil detachment equation and accounting for the rill erosion dynamic process is applied. Field measurements carried out for seven natural rainfall events occurring at the plots of the Sparacia experimental station, southern Italy, are used for indirectly measuring the rill erodibility of the investigated soil. This method needs only the knowledge of the geometric characteristics of the rills at the end of the erosion event and the event duration. The method was also tested by using the Water Erosion Prediction Project database, and this analysis showed that a reliable value of rill erodibility can be obtained by the proposed Equation 18 . The proposed method has the following advantages: (i) to be applicable at field scale, being more realistic than the laboratory scale because the soil structure is not destroyed, and (ii) to avoid the disadvantages of field experiments such as the cumbersome experimental set‐up and the large volume of water inflow needed. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献