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971.
Aerococcus viridans, the causative agent of the disease gaffkemia, was a major cause of mortality in lobsters (Homarus americanus) held in tidal impoundments during the 1970s and 1980s. Despite reports of an increase in the mortality of lobsters during impoundment, and the widespread prophylactic use of oxytetracycline against A. viridans, this bacterium has not been detected in active disease surveillance of the Maine postcapture lobster population. However, Photobacterium indicum may be an emerging opportunistic pathogen of stressed lobsters. An acute toxicity trial was conducted as a rapid screening procedure for the potential future use of the antibiotic florfenicol. Based on the results of this experiment, florfenicol appears to be well tolerated in adult H. americanus by intrapericardial injection at the 10-100 mg/kg dose. Oxytetracycline dihydrate is contraindicated by intrapericardial injection at the 10-100 mg/kg dose. 相似文献
972.
973.
Nicolas Desoignies Celine Stocco Claude Bragard Anne Legrève 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(1):27-38
The understanding of the molecular biology of Polymyxa betae, the protist vector of Beet necrotic yellow vein virus, remains limited because of the obligate nature of this root endoparasite and the limited data on the genome of Beta vulgaris, its most common host plant. The aim of this work was to assess the infection of P. betae in Arabidopsis thaliana in order to learn more about the P. betae genome and its interaction with the host. The susceptibility of a set of ecotypes of various origins to a monosporosorus
and aviruliferous isolate of P. betae was analyzed in a series of bioassays conducted under controlled conditions. P. betae was detected in roots of A. thaliana using light microscopy and PCR. The infection severity was relatively low in this species compared with B. vulgaris, but the different stages of the life cycle were present. The phenotype of P. betae in A. thaliana root cells differed from the phenotype in B. vulgaris: the spore-forming phase was more prevalent in comparison with the sporangial phase, and the sporosori contained a lower number
of spores. The compatible interaction between P. betae and A. thaliana obtained after the inoculation of zoospores and optimal conditions for the development of P. betae provide a new model system that can be used to improve the knowledge on the P. betae genome and on the mechanisms of the spore-forming phase of P. betae. 相似文献
974.
Marie-Liesse Vermeire Lucie Kablan Marc Dorel Bruno Delvaux Jean-Michel Risède Anne Legrève 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(4):621-630
Silicon (Si) is known to reduce the incidence of pathogens on many plants. Little information is available on the potential
positive effects of Si on the susceptibility of banana (Musa acuminata) to pathogens. Root-rot fungi of the genus Cylindrocladium have been reported, along with endoparasitic nematodes, to be the causal agent of toppling disease and severe yield loss.
The objective of this study was to determine the effects of Si supply on Cylindrocladium spathiphylli infection on banana. Plantlets inoculated by dipping the root system in a conidial suspension of the pathogen were grown
on a desilicated ferralsol and amended, or not, with 2 mM of soluble Si under greenhouse conditions in Guadeloupe. The root
lesion severity was evaluated using the image analysis program WinRHIZO 7, 14 and 21 days after inoculation. A reduction of
about 50% of root necrosis was observed 14 days after inoculation for the Si-supplied plants compared with those not supplied
with Si. The Si amendment also alleviated growth reduction caused by the pathogen. These results suggest that Si could have
a positive effect on banana resistance to C. spathiphylli and provide an environmentally friendly alternative to pesticides for the integrated control of an important crop disease. 相似文献
975.
976.
977.
Many biological processes are regulated through the selective dephosphorylation of proteins. Protein serine-threonine phosphatases are assembled from catalytic subunits bound to diverse regulatory subunits that provide substrate specificity and subcellular localization. We describe a small molecule, guanabenz, that bound to a regulatory subunit of protein phosphatase 1, PPP1R15A/GADD34, selectively disrupting the stress-induced dephosphorylation of the α subunit of translation initiation factor 2 (eIF2α). Without affecting the related PPP1R15B-phosphatase complex and constitutive protein synthesis, guanabenz prolonged eIF2α phosphorylation in human stressed cells, adjusting the protein production rates to levels manageable by available chaperones. This favored protein folding and thereby rescued cells from protein misfolding stress. Thus, regulatory subunits of phosphatases are drug targets, a property used here to restore proteostasis in stressed cells. 相似文献
978.
Griffitts JS Haslam SM Yang T Garczynski SF Mulloy B Morris H Cremer PS Dell A Adang MJ Aroian RV 《Science (New York, N.Y.)》2005,307(5711):922-925
The development of pest resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins used in transgenic and organic farming. Here, we demonstrate that (i) the major mechanism for Bt toxin resistance in Caenorhabditis elegans entails a loss of glycolipid carbohydrates; (ii) Bt toxin directly and specifically binds glycolipids; and (iii) this binding is carbohydrate-dependent and relevant for toxin action in vivo. These carbohydrates contain the arthroseries core conserved in insects and nematodes but lacking in vertebrates. We present evidence that insect glycolipids are also receptors for Bt toxin. 相似文献
979.
Iron isotope fractionation and the oxygen fugacity of the mantle 总被引:3,自引:0,他引:3
Williams HM McCammon CA Peslier AH Halliday AN Teutsch N Levasseur S Burg JP 《Science (New York, N.Y.)》2004,304(5677):1656-1659
The oxygen fugacity of the mantle exerts a fundamental influence on mantle melting, volatile speciation, and the development of the atmosphere. However, its evolution through time is poorly understood. Changes in mantle oxidation state should be reflected in the Fe3+/Fe2+ of mantle minerals, and hence in stable iron isotope fractionation. Here it is shown that there are substantial (1.7 per mil) systematic variations in the iron isotope compositions (delta57/54Fe) of mantle spinels. Spinel delta57/54Fe values correlate with relative oxygen fugacity, Fe3+/sigmaFe, and chromium number, and provide a proxy of changes in mantle oxidation state, melting, and volatile recycling. 相似文献
980.
Laitinen T Polvi A Rydman P Vendelin J Pulkkinen V Salmikangas P Mäkelä S Rehn M Pirskanen A Rautanen A Zucchelli M Gullstén H Leino M Alenius H Petäys T Haahtela T Laitinen A Laprise C Hudson TJ Laitinen LA Kere J 《Science (New York, N.Y.)》2004,304(5668):300-304
Susceptibility to asthma depends on variation at an unknown number of genetic loci. To identify susceptibility genes on chromosome 7p, we adopted a hierarchical genotyping design, leading to the identification of a 133-kilobase risk-conferring segment containing two genes. One of these coded for an orphan G protein-coupled receptor named GPRA (G protein-coupled receptor for asthma susceptibility), which showed distinct distribution of protein isoforms between bronchial biopsies from healthy and asthmatic individuals. In three cohorts from Finland and Canada, single nucleotide polymorphism-tagged haplotypes associated with high serum immunoglobulin E or asthma. The murine ortholog of GPRA was up-regulated in a mouse model of ovalbumin-induced inflammation. Together, these data implicate GPRA in the pathogenesis of atopy and asthma. 相似文献