Early hepatic immune response in rats infected with Fasciola hepatica

We investigated the phenotype of the T cells (CD4+ and CD8+) that produced Th1 (IFN-gamma) and Th2 cytokines (IL-4 and IL-10) during the firsttwo weeks of experimental fasciolosis in rats. We also followed the kinetics of the cytokine and proliferative responses of hepatic mononuclear cells (HMNC) over the same period. We found that HMNC were more numerous in the infected animals than in the controls. The percentage of CD4+ cells increased significantly after infection, whereas the percentage of CD8+ cells did not change. Moreover, the frequency of the cells producing (CP) cytokine changed after infection. The frequency of CP IFN-gamma on 7 days postinfection (pi) was similar to that in control animals. However, the frequency of CP IFN-gamma was clearly lower on day 14 pi, whereas the frequency of CP IL-4 and CP IL-10 had increased. The CP IL-10-were mostly CD4+. Mitogenic stimulation (phorbol myristate acetate/ionomycin) of HMNC led to an increase in the amounts of the Th2 cytokines in the supernatant on days 7 and 14 pi, with the increase more pronounced on day 14. In contrast, IFN-gamma levels also increased by day 7 pi but then decreased to below control levels by day 14. In addition, HMNC proliferation in response to mitogen followed a similar pattern to IFN-gamma production. These findings suggested that, during the first 2 weeks of infection, F hepatica induced a transient ThO cytokine profile followed by downregulation of the cellular response and the induction of a Th2 cytokine profile.     

Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats

The in situ distribution of NK cells in rat liver during the first 28 days of an experimental infection with F hepatica was investigated. NK cells were distributed homogeneously throughout the hepatic parenchyma in uninfected animals. The total number of hepatic mononuclear cells increased significantly following infection, but the proportion of NK cells did not change. After infection, these cells were found around the portal space, around the centrolobular vein, in the periportal fibrosis and in the band of collagen. However, no NK cells could be detected in or around the granuloma during infection. The frequency of both I L-2- and IFNgamma-producing NK cells was higher on day 7 postinfection (pi) but only the percentage of IFNgamma -CD161+ subsets remained elevated thereafter, whereas the percentage of both IL-2+CD161+ and IL-4+CD161+ subsets returned to the baseline. The number of CD161+IL10+ cells did not change significantly. These results suggest that NK cells could be another source for the early production of IFNgamma but provide no evidence that these cells are involved in early events associated with granuloma formation.     

Ethoxylated rapeseed oil derivatives as novel adjuvants for herbicides

Ethoxylates of rapeseed oil and of methylated rapeseed oil were synthesized and tested as adjuvants for 2,4-D and phenmedipham. Provided they had less than 6 units of ethylene oxide (EO), 1.0 to 10 g litre(-1) ethoxylates in water induced droplet spreading on barley leaves. In an acetone-based medium all derivatives strongly promoted the foliar uptake of 2,4-D, with no clear influence of the ethoxylation degree. In the same medium there was a negative influence of ethoxylate chain length on the foliar uptake of phenmedipham. In a water-based medium, phenmedipham applied with rapeseed oil emulsified with ethoxylated (20 EO) rapeseed oil displayed uptake rates close to a commercial preparation. The same was true for phenmedipham applied with ethoxylated (2 EO) methylated rapeseed oil. In bioassays, phenmedipham prepared with methylated rapeseed oil emulsified with ethoxylated (20 EO) rapeseed oil was as efficacious on barley as a commercial formulation. The same was true for phenmedipham prepared with ethoxylated (2 EO) methylated rapeseed oil. However, neither rapeseed oil nor methylated rapeseed oil emulsified with ethoxylated (2 EO) methylated rapeseed oil conferred good efficacy to phenmedipham. Hence, ethoxylated rapeseed oil derivatives are promising adjuvants or formulants for herbicides.     

Moisture content-water potential relationship of red pine sapwood above the fiber saturation point and determination of the effective pore size distribution

Summary The pressure membrane and pressure plate techniques were used to establish the moisture content-water potential (M-) relationship of red pine (Pinus resinosa Ait.) sapwood in desorption above the fiber saturation point. The moisture content-water potential relationship is required for the development of a model of drying considering the gradient of water potential as the driving force of moisture in wood. This relationship was established at 18, 56 and 85 °C for radial desorption. The results obtained demonstrate that water potential increases with temperature T at a given moisture content M. There is no significant variation of /T with temperature. Also, there is no plateau at intermediate moisture contents as was the case for the M- relationship of aspen sapwood established in a previous work. The effective integral and differential pore size distributions inferred from the M- relationship are also presented. The largest proportion of effective pore openings was found for a radius of 0.2 m. This value can be related to the pit membrane openings of red pine.This research project is currently supported by the Natural Sciences and Engineering Research Council of Canada under grant no. OGP0121954     

Chemical modification and wetting of medium density fibreboard panels produced from fibres treated with maleated polypropylene wax

Medium density fibreboard (MDF) was produced from fibres treated with maleated polypropylene wax. The objectives of this study were to investigate the effect of fibre treatment with maleated polypropylene wax on the advancing and receding contact angle and wicking of MDF panels by the Wilhelmy plate method; to verify the chemical reactions occurring between fibres and maleated polypropylene wax by infrared spectroscopy, and to verify if there are relationships with the water absorption of panels. Three maleated polypropylene wax contents (0, 3, 5%) and two resin types (urea–formaldehyde, UF and melamine–urea–formaldehyde, MUF) were considered in this study. Our results showed that the treatment increased the advancing contact angle of panels bonded with UF resin. The receding contact angles of MDF panels produced from treated fibres with 5% maleated polypropylene wax content were also increased by the treatment. Also, the wicking was reduced by the treatment independent of the maleated polypropylene wax content. Reductions of the wicking were 59% for panels bonded with UF resin and 73% for panels bonded with MUF. Relationships between the wicking and water absorption were observed. Chemical analysis realized by infrared spectroscopy did not detect the presence of an esterification reaction between wood fibres and anhydride groups of the maleated polypropylene wax. This suggests, therefore, that an esterification reaction did not take place or that chemical modifications were so small that they are not visible by infrared spectroscopy.     

Characterization of juvenile wood to mature wood transition age in black spruce (Picea mariana (Mill.) B.S.P.) at different stand densities and sampling heights

The radial pattern of both maximum ring density and ring area of 36 black spruce trees were used to determine the transition age from juvenile wood to mature wood. The data were obtained by X-ray densitometry and both segmented linear and polynomial regressions were used to point out the age of the juvenile wood boundary. Three stand densities (1,790, 2,700 and 3,400 stems/ha) and three sampling heights (2.4, 5.1 and 7.8 m) were studied. Although maximum ring density and ring area presented similar radial patterns, they gave two significantly different results of transition ages. The maximum ring density over-estimated the transition age (17.6 years) in contrast to ring area (14 years). The results show that the transition from juvenile wood to mature wood occurred after 12 years at 7.8 m (versus 13.1 years at a height of 5.1 m, and 17.6 years at 2.4 m). Although transition age occurred later in the high stand density group (21 years), the difference was not significant between the three stand density groups. Nevertheless, transition age remains difficult to determine since no standard definition exists. The transition occurs over years, and most probably a transition wood exists between juvenile wood and mature wood. Estimation of the juvenile wood proportion in volume shows that it remains constant along the stem and it increases with stand density.     

Live fences in Ségou, Mali : an evaluation by their early users

Market-oriented production of gardening crops and cassava (Manihot utilissima) in the dry season is an increasingly frequent practice in Ségou, Mali. Traditionally, these crops are protected from roaming livestock with the help of dead fences. In order to provide a sustainable alternative to dead fences, the International Centre for Research in Agroforestry (ICRAF) began promoting the use of live fences, living trees planted closely together around a field plot. This study was conducted with the first farmers to use these live fences. These farmers expressed satisfaction with the protection offered by the trees and their ability to provide a variety of medicinal, economic, and food products. The form of land tenure, the social status of farmers within their families, and the availability of labour seem to be important factors in the decision to test the live fence. This raises questions about the accessibility of this technique and its possible contribution to the social and economic differentiation of its users. This revised version was published online in June 2006 with corrections to the Cover Date.     

Characterization of urea–formaldehyde resin penetration into medium density fiberboard fibers

The amount of UF resin penetration into fibers, used for the production of medium density fiberboard (MDF), is unknown. To evaluate the relationship between resin viscosity and resin penetration depth, an experimental procedure involving confocal laser scanning microscopy (CLSM) and a Toluidine Blue O staining system was performed. The results indicate that CLSM in combination with a Toluidine Blue O staining system is a good way to characterize UF resin penetration into wood fibers. The main penetration direction is toward the fiber lumen. For wet fibers, whose moisture content is about 88%, the effect of resin viscosity with a range of 80 cps – 340 cps on penetration is very similar, with all adhesives reaching or almost reaching the fiber lumen after 60 min at room temperature. For MDF industrial samples, the highest depth of penetration of the adhesive was attained in the second dryer stage. After the second dryer stage, the resin penetration into the fiber did not increase.     

INTER‐ AND INTRAFRACTION MOTION FOR STEREOTACTIC RADIOSURGERY IN DOGS AND CATS USING A MODIFIED BRAINLAB FRAMELESS STEREOTACTIC MASK SYSTEM

Precise and accurate patient positioning is necessary when doing stereotactic radiosurgery (SRS) to ensure adequate dosing to the tumor and sparing of normal tissues. This prospective cross‐sectional study aimed to assess feasibility of a commercially available modified frameless SRS positioning system for use in veterinary radiotherapy patients with brain tumors. Fifty‐one dogs and 12 cats were enrolled. Baseline and verification CT images were acquired. The verification CT images from 32 dogs and five cats had sufficient images for fusion to baseline CT images. A rigid box‐based fusion was performed to determine interfraction motion. Forty‐eight dogs and 11 cats were assessed for intrafraction motion by cine CT. Seventy percent of dogs and 60% of cats had interfraction 3D vector translational shifts >1 mm, with mean values of 1.9 mm in dogs, and 1.8 mm in cats. In dogs muscle wasting was weakly correlated with translational shifts. The maximum angular interfraction motion observed was 6.3° (roll), 3.5° (pitch), and 3.3° (yaw). There was no correlation between angular interfraction motion and weight, brachycephaly, or muscle wasting. Fifty‐seven percent of dogs and 50% of cats had respiration‐related intrafraction motion. Of these, 4.5% of dogs and 10% of cats had intrafraction motion >1 mm. This study demonstrates the modified Brainlab system is feasible for SRS in dogs and cats. The smaller cranial size and difference in anatomy increases setup uncertainty in some animals beyond limits usually accepted in SRS. Image‐guided positioning is recommended to achieve clinically acceptable setup accuracy (<1 mm) for SRS.     

Changes in red wine soluble polysaccharide composition induced by malolactic fermentation

The polysaccharide content of wine is generally assumed to originate from grapes and yeasts, independent of bacterial metabolism, except for the action of certain spoilage species. This study shows that malolactic fermentation (MLF) significantly modifies the soluble polysaccharide (SP) concentration of various red Bordeaux wines. Wines with the highest initial SP concentration go on to present decreased SP concentration, whereas those with the lowest initial SP concentration rather go on to have a higher SP concentration after MLF. These tendencies were observed whatever the Oenococcus oeni strain (indigenous or starter) used for MLF. Neutral and charged SPs were affected, but to a degree that depended on the microorganisms driving the MLF. The SP modifications were directly linked to bacterial development, because non MLF controls did not present any significant change of SP concentration.