Studies on Models,Patterns and Require-ments of Digestible Amino Acids for Layers by Nitrogen Metabolism

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Human Face Location Based on Motion Information in a Complex Background

This paper proposes an improvement for the traditional Optical Algorithm, and presents a new way to image segmentation in a complex background. In addition, combined with the neural network, the system can locate the possible human faces successfully by means of two-step location model. In our system, the searching and locating of the human face is the most important stage. According to this, the authors adopt the two-step way to run, firstly they take up the segmentation of the candidate human face areas and then the accurate face locating based on the neural network is used. This algorithm is fast and robust. Experimental results with real scene images are given out there, and all these prove that two-step method gains many advantages in the course of human face location with motion information, such as real-time, robustness and practicality. In addition, the proposed system is also the fundamental and important part of the perfect human face recognition system.     

用玉米圆斑病菌毒素筛选抗病单倍体胚性细胞无性系研究初报

本试验用圆斑病菌（Helminthosporium carbonum）的粗提毒素处理单倍体玉米胚性细胞团，以未处理的细胞团为对照，用HC-毒素和活菌的 分子孢子接种细胞团鉴定，初步筛选出抗圆斑病菌毒素和抗圆斑菌的细胞团。在筛选体系中，初步认为用高浓度毒素（20-25%毒素处理）体系的效果较理想。通过组织培养技术诱发玉米抗病突变体可能为玉米抗病育种开辟新的途径。     

Synergistic effects of tropical cyclones on forest ecosystems:a global synthesis

Tropical cyclones are large-scale strong wind disturbance events that occur frequently in tropical and subtropical coastal regions and often bring catastrophic physical destruction to ecosystems and economic disruption to societies along their paths. Major tropical cyclones can infrequently move into the midaltitudes and inland areas. Ecologically, tropical cyclones have profound impacts on diversity, structure, succession and function of forest ecosystems. The ecological effects are both dramatic and subtle. The dramatic effects can be visible, noticeable and to some extent predictable over the short-term and relatively well documented in the literature. However, the subtle effects are often invisible, complex and at smaller scale relatively unpredictable in the long-term. Many factors, meteorologic, topographic and biologic, simultaneously interact to influence the complexity of patterns of damage and dynamics of recovery. I present a global synthesis on the effects of tropical cyclones on forest ecosystems and the complexity of forest responses, with particular attention on the response to large hurricanes in the neotropics and the temperate North America, and strong typhoons on the subtropical and temperate forests in the East and Southeast Asia. Four major aspects provide on organizational framework for this synthesis:(1) consistent damage patterns,(2) factors that influence response patterns and predict damage risks,(3) complexity of forest responses and recovery, and(4) the long-term effects. This review reveals highly variable and complex effects of tropical cyclones on forest ecosystems. A deep understanding of the synergistic effects of tropical cyclones is essential for effective forest management and biodiversity conservation.     

模拟降雨条件下土壤结皮的发展和聚丙烯酰胺对黄土上结皮形成的影响

This study evaluated the morphological characteristics and dynamic variation in characteristics of soil crust and iden-tified the relationships between soil crust and splash erosion under simulated rainfall.The effect of polyacrylamide (PAM) on soil aggregate stabilization and crust formation was also investigated.A laboratory rainfall simulation experiment was carried out using soil sample slices.The slices were examined under a polarized light microscopy and a scanning electron microscope (SEM).The results revealed that the soil crusts were thin and were characterized by a greater density,higher shear strength,finer porosity,and lower saturated hydraulic conductivity than the underlying soil.Two types of crusts,i.e.,structural and depositional crusts,were observed.Soil texture was determined to be the most important soil variable influ-encing surface crust formation;depositional crust formation was primarily related to the skeleton characteristics of the soil and happened when the soil contained a high level of medium and large aggregates.The crust formation processes observed were as follows:1) The fine particles on the soil surface became spattered,leached,and then rough in response to raindrop impact and 2) the fine particles were washed into the subsoil pores while a compact dense layer concurrently formed at soil surface due to the continual compaction by the raindrops.Therefore,the factors that influenced structural crust formation were a large amount of fine particles in the soil surface,continual impact of raindrops,dispersion of aggregates into fine particles,and the formation of a compact dense layer concurrently at the soil surface.It was concluded that the most important factor in the formation of soil crusts was raindrop impact.When polyacrylamide (PAM) was applied,it restored the soil structure and greatly increased soil aggregate stabilization.This effectively prevented crust formation.However,this function of PAM was not continuously effective and the crust reformed with long-term rainfall.In conclusion,this study showed that soil micromorphological studies were a useful method for evaluating soil crust formation.     

Preparation of anti-pefloxacin antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of pefloxacin residue in chicken liver

Pefloxacin has been increasingly used in veterinary medicine to treat microbial infections. To avoid using a labor-intensive instrumental method to detect the residue of pefloxacin in food, a simple and convenient indirect competitive enzyme-linked immunosorbent assay method has been developed in this study. The antibody generated from immunogen cationized bovine serum albumin-pefloxacin showed high sensitivity toward pefloxacin with an IC50 value of 6.7 ppb in buffer and was suitable for a screening assay to detect the residue of pefloxacin in food products. The antibody has been assessed using rapid enzyme immunoassays to exploit its specificity. The antibody prepared shows cross-reactivity with a few other (fluoro)quinolones including fleroxacin (116%), enrofloxacin (88%), and ofloxacin (10%). The assay measured drug residue in chicken liver spiked with pefloxacin with an interassay coefficient of variation of 13.6% or less and an intra-assay coefficient of variation of 10.9% or less. The average recovery rates at 0.5, 5, 10, 50, and 100 ppb were in the range of 86-106% for interassay and in the range of 87-103% for intra-assay, respectively.     

Effect of constitutive expression of porcine IGFBP-3 on proliferation and differentiation of L6 myogenic cells

We have previously shown that exogenous recombinant porcine IGFBP-3 (rpIGFBP-3) suppresses proliferation and differentiation of L6 myogenic cells in an IGF-I-dependent manner and suppresses proliferation of L6 myogenic cells via an IGF-I-independent mechanism. In order to assess the effects of endogenously produced IGFBP-3, we have transfected L6 myogenic cells with a pEF6/V5 vector containing pIGFBP-3 cDNA under the control of the human elongation factor 1alpha (hEF-1alpha) promoter and with the empty vector. We have isolated a cell population that constitutively produces porcine IGFBP-3 (tL6 cells) and a stable mock transfected cell population containing the empty vector (mtL6 cells). Constitutive expression of IGFBP-3 slightly reduced the expression of IGFBP-5 but had no effect on IGFBP-4 production by L6 myogenic cells. Immunoneutralization of IGFBP-3 increased both IGF-I- and Long-R3-IGF-I-stimulated proliferation of tL6 cells (58 and 33%, respectively) (P<0.01). These data indicate endogenous pIGFBP-3, like exogenous rpIGFBP-3, suppresses the proliferation of L6 myogenic cells via both IGF-I-dependent and -independent pathways. Immunoneutralization of IGFBP-3 also increased IGF-I-stimulated differentiation (21%, P<0.05) but had no effect on Long-R3-IGF-I stimulated differentiation of tL6 myogenic cells. Results indicate that exogenous and endogenous IGFBP-3 affect proliferation and differentiation of L6 myogenic cells in a similar way. Immunohistochemical localization data reveal that pre-incubation with anti-pIGFBP-3 dramatically reduces the level of intracellular IGFBP-3 in tL6 myogenic cells indicating that endogenously produced IGFBP-3 must first be secreted before it is internalized and that anti-pIGFBP-3 prevents internalization of IGFBP-3. TL6 and mtL6 cells provide a good system to further investigate the mechanisms by which IGFBP-3 affects proliferation and differentiation of myogenic cells.     

Electromagnetic induction method for detecting neglected loading of metal parts

In industrial production, neglected loading of metal parts is very normal. It not only causes the quality reduction of products, but also affects production rate. Based on the theory of electromagnetic induction, by supervising the assemblage process of metal parts, it is able to count the number of assembled metal parts so that automatic online monitoring of neglected loading can be achieved. The feasibility of using the eddy current method to monitor the motions of metal parts is analyzed theoretically and MATLAB is utilized to simulate. On the basis of theoretical analysis, the specific eddy current type transducer, the appropriate signal-processing circuits and the software are designed. A testing system is established for analog. This method can effectively solve the problem of neglected loading by artificial factors, save the costs of factories, raise the FPY of products.     

Single reconstituted neuronal SNARE complexes zipper in three distinct stages

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins drive membrane fusion by assembling into a four-helix bundle in a zippering process. Here, we used optical tweezers to observe in a cell-free reconstitution experiment in real time a long-sought SNARE assembly intermediate in which only the membrane-distal amino-terminal half of the bundle is assembled. Our findings support the zippering hypothesis, but suggest that zippering proceeds through three sequential binary switches, not continuously, in the amino- and carboxyl-terminal halves of the bundle and the linker domain. The half-zippered intermediate was stabilized by externally applied force that mimicked the repulsion between apposed membranes being forced to fuse. This intermediate then rapidly and forcefully zippered, delivering free energy of 36 k(B)T (where k(B) is Boltzmann's constant and T is temperature) to mediate fusion.