Flumorph Is a Novel Fungicide That Disrupts Microfilament Organization in Phytophthora melonis

ABSTRACT The mechanism of the effects of flumorph (a novel fungicide) was investigated by analyzing alterations of hyphal morphology, cell wall deposition patterns, F-actin organization, and other organelles in Phytophthora melonis. Calcofluor white staining suggested that flumorph did not inhibit the synthesis of cell wall materials, but disturbed the polar deposition of newly synthesized cell wall materials during cystospore germination and hyphal growth. After exposure to flumorph, zoospores were able to switch into cystospores accompanied with the formation of a cell wall, whereas cystospores failed to induce the isotropic-polar switch and did not produce germ tubes but continued the isotropic growth phase. In flumorph-treated hyphae, the most characteristic change was the development of periodic swelling ("beaded" morphology) and the disruption of tip growth. Newly synthesized cell wall materials were deposited uniformly throughout the diffuse expanded region of hyphae, in contrast to their normal polarized patterns of deposition. These alterations were the result of F-actin disruption, identified with the fluorescein isothiocynate (FITC)-phalloidin staining. The disruption of F-actin also was accompanied by disorganized organelles: each swelling of subapical hyphae was associated with a nucleus. Vesicles did not undergo polarized secretion to the apical hyphae, but diffused around nuclei for the subapical growth; thus, the cell wall was thickened with periodic expansion along the hyphae. Upon removing flumorph, normal tip growth and organized F-actin were observed again. These data, as well as data published earlier, suggest that flumorph may be involved in the impairment of cell polar growth through directly or indirectly disrupting the organization of F-actin. The primary site of action by flumorph in the disruption of the F-actin organization is under investigation.     

NEW INSIGHTS INTO THE PHYLOGENY AND SPECIATION OF KUMQUAT (FORTUNELLA SPP.) BASED ON CHLOROPLAST SNP,NUCLEAR SSR AND WHOLE-GENOME SEQUENCING

● Fortunella genus consists of two populations: cultivated kumquat and wild Hong Kong kumquat. ● Artificial selection might involve in the origin of cultivated Fortunella species. ● A hypothesis for the differentiation and speciation of Fortunella species is proposed. Kumquat (Fortunella spp.) is a fruit and ornamental crop worldwide due to the palatable taste and high ornamental value of its fruit. Although Fortunella is classified into the economically important true citrus fruit tree group together with Citrus and Poncirus, few studies have been focused on its evolutionary scenario. In this study, analysis of five chloroplast loci and 47 nuclear microsatellites (nSSR) loci from 38 kumquat and 10 citrus accessions revealed the independent phylogeny of Fortunella among citrus taxa, and that Fortunella mainly comprises two populations: CUL, cultivated Fortunella spp. (F. margarita, F. crassifolia and F. japonica); and HK, wild Hong Kong kumquat (Fortunella hindsii). Genomic analysis based on whole-genome SNPs indicated that the allele frequency of both pupations deviated from the neutral selection model, suggesting directional selection was a force driving their evolutions. CUL exhibited lower genomic diversity and higher linkage strength than HK, suggesting artificial selection involved in its origin. A high level of genetic differentiation (Fst = 0.364) was detected and obviously asynchronous demographic changes were observed between CUL and HK. Based on these results, a new hypothesis for the speciation of Fortunella is proposed.     

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised intercaruncular areas （IC）. In order to better understand aglandular areas called caruncular （C）, and intensely glandular the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins （DEPs） （P 〈 0.05）, functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

胡子鲶网箱养殖试验

胡子鲶俗称塘鱼，属胡子鲶科．胡子鲶属。广东省本地种称本地塘，原系野生鱼类，栖息于河川下游和田野坑渠、池塘的石缝暗沟处，喜群居。塘鱼贪食，以摄食动物性饵料为主，属底栖腐屑食性的杂食鱼类。其肉味鲜美、营养丰富，市场价格较高。成为人们竞相研究开发的养殖对象。目前，本地塘鱼的养殖仅限于池塘单养或套养，成活率较低，生长速度慢，难起捕。用网箱养殖本地塘鱼尚无成功的技术报道。我公司网箱养殖试验场在今年六至九月间，用网箱成功地养殖本地塘鱼1500余尾，四百多斤。现将我场的试验情况介绍如下。1．材料和方法1．1材料使用…     

Finite Element Analysis of Concrete Structures Strengthened with Carbon Fiber Sheet

Stress redistributes after the concrete structures strenthened with carbon fiber reinforced polymer sheet. It is a question of secondary loading.Many scholars have studied it and got a lot of achievements.This paper summarizes the methods and analyzes their advantage and shortcoming. Based on it, a new method is proposed.The method is developing in large-scale general software-Ansys by using adding new elements, constitutive relationship, failure criterion,and so on.The calculation formula agrees with the experimental data.     

FEM Dynamical Analysis of the Reinforced Concrete Beam with Bonding Steel Plate

In order to researchmechanical capability of the reinforced concrete beam with bonding steel plate, adopting separatemethod to establish finite element model of the reinforced concrete beam with bonding steel plate, using FEM to study dynamical characteristics of the model, have got good results which can not be easily got using traditional resolution method, which can give referenceto study dynamical characteristics of the reinforced concrete beam with bonding steel plate.     

Pretreatment of Coke Oven Waste Water with Blast Furnace Sludge Method and Blast Furnace Sludge Plus Scrap Iron Method

Coke oven waste water is respectively pretreated by blast furnace sludge method and blast furnace sludge plus iron scrap method, the effects of pH and the quantity of materials and treatment time on the removal efficiency of COD are analyzed. The results indicate that blast furnace sludge plus iron scrap method is superior to blast furnace sludge method .The treatment time and pH can greatly affect on the removal efficiency of COD using blast furnace sludge plus iron scrap method. The treatment time after 30 minutes and pH have no remarkable effects the removal efficiency of COD using blast furnace sludge method. The removal efficiency of COD increases with the increase of blast furnace sludge and iron scrap, but the increase rate decreases gradually.     

An Evaluating Method of Human Resource Value Based on Rough Set

As the development of knowledge economic , human resource has been the core of the world economic .So searching the reasonable evaluating and managing methods becomes one of the hot issues which have been concerned by people . Traditional evaluating methods of human resource are simple and direct , but they greatly depend on people's subjective judgement and experience . In order to overcome the default , this paper puts forward the method of synthetically evaluation on rough sets, which takes advantage of the rough sets theory, the concept of information entropy and relative analysis to filter the original indexes, then to standardize the importance degree of every index and get the weight of every index. Synthetically valuation model and corresponding arithmetic are summed up. Finally the sample illuminates the validity and feasibility of this method.     

有机酸对高岭石, 针铁矿和水铝英石吸附镉的影响

Effects of organic acids （oxalic, acetic, and citric） on adsorption characteristics of Cadmium （Cd） on soil clay minerals （kaolinite, goethite, and bayerite） were studied under different concentrations and different pH values. Although the types of organic acids and minerals were different, the effects of the organic acids on the adsorption of Cd on the minerals were similar, i.e., the amount of adsorbed Cd with an initial solution pH of 5.0 and initial Cd concentration of 35 mg L^-1 increased with increasing concentration of the organic acid in solution at lower concentrations, and decreased at higher concentrations. The percentage of Cd adsorbed on the minerals in the presence of the organic acids increased considerably with increasing pH of the solution. Meanwhile, different Cd adsorption in the presence of the organic acids, due to different properties on both organic acids and clay minerals, on kaolinite, goethite, or bayerite for different pHs or organic acid concentrations was found.     

2-fluoroabscisic acid analogues: their synthesis and biological activities

Fluorine was introduced into the 2-position of the side chain of abscisic acid (ABA) analogues by Wittig reaction of alpha-ionone derivatives with ethyl triethylphosphono-2-fluoroacetate. The effects of the fluorinated analogues were evaluated on inhibition of cress seed germination and inhibition of gibberellin-inducible alpha-amylase induction in embryoless barley half-seeds. (2E, 4E)-2-Fluoro-5-(1'-hydroxy-2',6', 6'-trimethyl-2'-cyclohexen-1'-yl)-3-methyl-2,4-pentadienoic acid (5b) showed potent inhibitory activity at the same level as ABA in the cress seed germination test, and 5b also inhibited gibberellin-inducible alpha-amylase induction at 4 x 10(-)(6), 3 times the concentration of ABA (1 x 10(-)(6)) for 50% inhibition of alpha-amylase production. 5b also showed dehydrin induction activity. These results indicate that fluorinated ABA analogues mimic ABA action and can be a lead for a plant growth regulator which regulates plant growth or protects plants from environmental stresses.