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71.
原发性肾病综合征患者血、尿γ-干扰素的检测及其临床意义 总被引:2,自引:0,他引:2
目的:为进一步阐明γ—干扰素(IFN—γ)在原发性肾病综合征(PNS)发生发展中的作用及检测IFN—γ的临床诊断价值。方法:对32例PNS患者及10例正常人血清、尿液IFN—γ的水平进行检测,并结合PNS病理类型及其他临床指标进行分析。结果:系膜增生性肾炎(MesPGN)患者尿IFN—γ水平显著高于正常对照组,而非增生性肾炎组却与对照组差异无显著性;其次,尿IFN—γ水平与尿纤维蛋白(原)降解产物(FDP)浓度呈正相关;但与血肌酐(Ser)、血清肌酐清除率(Ccr)、24h尿蛋白定量(24hUPQ)无相关性。血清IFN—γ水平各组问差别无统计学意义。结论:IFN—γ参与增生型PNS免疫性肾损伤过程,并可能通过局部方式起作用,尿IFN—γ检测有可能作为区分增生性肾炎和非增生性肾炎的指标。 相似文献
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Daniel J. Burba DVM Michael A. Collier DVM Lawrence E. Default PhD Olivia Hanson-Painton PhD Harold C. Thompson Jr. Claude L. Holder 《Journal of Equine Veterinary Science》1993,13(12):696-703
The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (3H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. 3H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean 3H-PSGAG concentrations peaked at 2 hours post-injection. 3H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in 3H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.
HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.
3H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan. 相似文献
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In vitro viability of cryopreserved equine embryos following different freezing protocols. 下载免费PDF全文
P Poitras P Guay D Vaillancourt N Zidane M Bigras-Poulin 《Canadian journal of veterinary research》1994,58(4):235-241
The main objective of this study was to evaluate two freezing protocols and the effect of agar embedding on survival of day 6.5 equine embryos. A total of 133 embryos were used, in one group (n = 51), embryos were first embedded in agar before the freezing protocol was started. A freezing protocol to -30 degrees C or -33 degrees C was used before plunging embryos into liquid nitrogen (LN2). The embryos were thawed in water at 37 degrees C, evaluated and placed in culture. After 24 h culture, the embryos were evaluated for their morphology and development. No differences were observed between embryos plunged at -30 degrees or at -33 degrees C in LN2. The analysis of the morphology and development after thawing showed that the diameter and developmental stage at freezing correlated with embryo survival. Morula and early blastocyst stages of development were associated with better quality after freezing and thawing and had a better potential to survive after in vitro culture (p < 0.05) compared to more advanced stages. The agar failed to protect embryos from zona pellucida damage, but a tendency to prevent rupture was observed in larger embedded embryos. 相似文献
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通过对贵州省贞丰、桐梓、锦屏等若干县市林业生态建设监理、检查、造林实绩核查等工作的实践和研究,认为退耕还林工程与农村经济发展相结合,目前应加强以下工作:选择林业主导产业;林产业时间、空间的合理布局;树种、林种的结构优化;林业体制创新;实施相关配套工程。 相似文献
79.
应用现代文体学理论并结合语用学和认知语言学理论观点,尝试从字位与书写、词汇、句法层面描述和分析WTO英文协议的文体特征,希望对理解和翻译世界贸易法规和类似的经贸法规有所帮助。 相似文献
80.
Presentation of postweaning Escherichia coli diarrhea in southern Ontario, prevalence of hemolytic E. coli serogroups involved, and their antimicrobial resistance patterns 总被引:1,自引:0,他引:1 下载免费PDF全文
Rocio Amezcua Robert M. Friendship Catherine E. Dewey Carlton Gyles John M. Fairbrother 《Canadian journal of veterinary research》2002,66(2):73-78
Post-weaning Escherichia coli diarrhea (PWECD) in Ontario was investigated using a case-control study involving 50 Ontario nurseries. The clinical signs and the impact on productive parameters were determined by means of a producer survey. The hemolytic E. coli serogroups involved in PWECD (O149:K91:K88) were examined in this study. Based on a polymerase chain reaction test, the hemolytic E. coli from 82% of the case herds were positive for 3 enterotoxins (STa, STb, and LT), those from 12% of the case herds were positive for STb and LT only, and those from one herd (6%) were positive for 3 enterotoxins, as well as for verotoxin and F18 pili. The E. coli involved in disease were resistant to multiple antibiotics. Case farms commonly used a wide variety of antibiotics either in the feed or water, or as injectable drugs. The most common antibiotic used to treat PWECD on the study farms was apramycin, but evidence of resistance to this antibiotic was noted. The PWECD problem was commonly seen within a week of weaning but onset of diarrhea was reported as late as the grower-finisher stage. Growth rate was poorer in case herds and mortality was higher than in control herds, demonstrating that PWECD is an economically important disease in Ontario. 相似文献