扁穗牛鞭草种质遗传多样性的ISSR分析

用ISSR标记对来自中国西南地区(四川、重庆、贵州)的28份扁穗牛鞭草材料的遗传多样性进行了检测。从96个ISSR引物中共筛选出13个多态性明显、反应稳定的引物。28份材料的DNA共扩增出129条谱带,平均每个引物扩增出9.9条带,多态性条带比率达84.2%。材料间遗传相似系数为0.466~0.980,表现出丰富的遗传多样性。通过聚类分析和主成分分析,将28份扁穗牛鞭草分为两大类,同一地区的扁穗牛鞭草品种(系)基本聚在同一类,呈现出一定的地域性分布规律。     

Effect of advanced glycation end products on inflammation in cultured cardiomyocytes

AIM: To investigate the effect of advanced glycation end products on inflammation in cultured cardiomyocytes.METHODS: Primary cardiomyocytes were isolated from Sprague-Dawley neonatal (1 to 2 days old) rats ventricles.The insulin resistant cardiomyocyte model was established.Neonatal rat ventricular myocytes were exposed to AGEs for 24 hours.TNF-α mRNA and PPAR-γ mRNA expressions were determined by RT-PCR.Activation of NF-κB in the cells was examined by using immunocytochemistry.The ultrastructure of the cells was detected by transmission electron microscope.RESULTS: The exprssion of TNF-α mRNA and the activation of NF-κB increased,the expression of PPAR-γ mRNA decreased compared with control group (P<0.05).The differences among different AGE-BSA groups were significant (P<0.05).The numbers of chondriosome and smooth endoplasmic reticulum increased.CONCLUSION: AGEs significantly increase TNF-α mRNA expression and NF-κB activation,and restrain the expression of PPAR-γ mRNA.These data suggest that AGEs play an important role in the onset of diabetic cardiomyopathy.     

Purification and differentiation potentiality of fetal liver mesenchymal stem cells from mouse

AIM: To purify and investigate the differentiation potentials of fetal liver mesenchymal stem cells (flMSCs) from murine in vitro.METHODS: flMSCs from mouse fetuses at embryonic and fetal day (ED)13.5 or ED14.5 were isolated by adhering to plastic and passaged by modified method.Cell cycle and phenotype were analyzed by flow cytometry.The cell differentiation was induced by special induction media.The cells differentiated to adipose,cartilaginous and osteoid tissues were identified with oil red O,Toluid blue,alkaline phosphatease (ALP) and von Kossa’s staining.The cells differentiated to neural-like cells were detected by RT-PCR and immuno-staining.RESULTS: Fibroblast-like cells predominated in culture.(83.76±2.88)% of flMSCs stayed in the G₀/G₁ phases.Homogenous cells were positive for mesenchymal lineage markers CD44,CD29,but not for markers of hematopoietic cells CD45,CD11b.flMSCs were able to differentiate into adipogenic,chondrogenic,osteogenic and neurogenic cells.CONCLUSION: flMSCs can be purified by modified plastic-attachment method and have multiple differentiation,which is available to stem cell therapy for various diseases.     

不同灌溉方式对水稻根系生长的影响

采用根箱试验研究了间歇灌溉与淹灌两种灌溉方式对汕优10号根系生长的影响.结果表明,间歇灌溉处理具有更高的根系活力,较高的根系生物量和较深的根层分布,抽穗以后土表10 cm以下根系生物量比例高于淹灌处理.这可能是间歇灌溉条件下,稻株氮素吸收及利用率增强,后期干物质积累及产量提高的重要生理原因.     

不同退耕模式林草初夏小气候效应

通过对安塞县几种不同类型退耕还林还草模式,如混交林、人工灌木林、野生灌木林及林中革地进行了小气候观测,分析了不同退耕模式林草配置下的主要小气候特征,采用平行对比观测法定点定时观测了空气温湿度,土壤温度,水面蒸发并与对照裸地进行了对比.结果表明4种不同退耕模式林草配置在6月高温时期能显著降低空气温度和地表温度的日均值,提高空气湿度和土壤湿度,减少水面蒸发等,具有明显的调节功能.4种不同退耕模式日平均空气温度变化呈不对称的单峰曲线,较对照裸地可以降低0.41℃～1.2℃;0～20 cm各深度的土壤温度基本都是混交林＜人工灌木林＜野生灌木林＜林中草地＜对照裸地,混交林内地面最高温度为34.78℃,明显比对照裸地42.54℃低;空气相对湿度日动态呈"U"型变化,其中对照裸地相对温度日均值最低,混交林日均值最大,为55.2%,比对照裸地高3.95%;水面蒸发量为裸地最大,混交林及灌木林能有效减少水面蒸发,减少量较对照裸地可达50.54%和55.20%.     

梭梭幼苗根系分泌物提取方法的研究

为了探讨梭梭根系分泌物中的化学成分,本文采用水浸提取和循环灌溉两种方法对梭梭幼苗根系分泌物进行收集和提取,结合气相色谱-质谱联用分析技术,分析梭梭幼苗根系分泌物中组分和含量的差异。实验结果表明:循环灌溉收集的梭梭幼苗根系分泌物中检测到35种化合物,包括酚醌类、醛酮类、醇类、酸类、脂类和胺类化合物;硅烷化处理后,主要增加部分强极性有机酸,如苯甲酸。水浸提取梭梭幼苗根系分泌物的中性相组分共检测出27种化合物,但未检测到酚醌类化合物;碱性相组分共检测出23种化合物,醛酮类化合物种类减少,烷烃类化合物种类增加;酸性相组分中仅检测到10种化合物,以醇类化合物为主,占检出化合物总量的72.62%。收集梭梭幼苗根系分泌物循环灌溉是一种有效的方法。     

毛乌素沙地臭柏、油蒿细根生产动态及影响因子

在毛乌素沙地开发整治研究中心北试实验地选取了七块样地,并依据建群植被外貌特征划分为臭柏群落、臭柏灌丛、油蒿群落三类。用根钻对各样地一个生长季内的根系进行了五次取样,同时测量土壤水分、枝条生长长度、温湿度等一些气象因子。对活细根分级并称干重,发现臭柏、油蒿不同径级细根的季节动态不同,不同土层内相同径级的细根生产动态也不相同:①臭柏与油蒿D≤2 mm的细根生物量在4~5月份都有一个快速增长的阶段,随后增长格局各不相同。②臭柏灌丛中,1 mm相似文献   

基于气温、降水变化的南疆气候变化分析

近年来新疆气候尤其是南疆气候出现了一些引人注目的变化。本文采用南疆10个站点1955~2000年的气温和降水资料进行了研究,分别分析了南疆近50年来气温和降水的年际变化、季节变化,并对其进行了异常性分析。研究表明:在这45年里南疆气候已呈现出较明显的变暖变湿趋势。