In silico identification of cis-regulatory elements of phosphate transporter genes in rice (Oryza sativa L.)

Promoter sequences of 13 Phosphate Transporter genes of Oryza sativa L. (OsPTs) have been analyzed in silico to identify their cis-regulatory elements (CREs). The DNA sequences of these OsPT genes were mined from NCBI gene databases. MEGABLAST program was used to align these sequences to Rice Genome Database in obtaining their complete sequences. The upstream region (−1 to −400) of the complete gene sequences were analyzed using SIGNALSCAN program provided by PLACE database of cis-regulatory element motives. From this procedure, 153 types of CRE were identified. Four of these CREs were found on all of the OsPT genes: ARR1AT, CAATBOX1, CACTFTPPCA1, and DOFCOREZM. Among these CREs, CACTFTPPCA1 was found with 3 to 15 duplications in each cis-regulatory sequence. In addition, each OsPT gene has typical CREs that can only be found in the respective genes. The total number of these typical CREs is 54, and one of them was a binding site for a bHLH-like protein, CACGTGMOTIF or the G-box. Moreover, several E-boxes which also functioned as a binding site for a bHLH-like protein were identified in all OsPTs except in OsPT1, OsPT6, and OsPT8. There were significant correlations (p < 0.05) between mRNA levels of OsPT1 to OsPT11 in rice root with or without inoculation of Glomus intradices reported by Paszkowskidagger et al. (2002) and the duplication numbers of ARR1AT, CAATBOX1, CACTFTPPCA1, CURECORECR, and WRKY71OS.     

Effect of Microwave Heating of Kafirin on the Functional Properties of Kafirin Films

To improve the functional properties of cast kafirin films, dry kafirin, extracted with an aqueous ethanol‐based solvent at 70°C, was microwave‐heated. No effect on film tensile properties was found. Two strategies were employed to improve the effect of microwaving: extraction of kafirin using an aqueous tert‐butanol‐based solvent at ambient temperature to minimize temperature‐induced denaturation and wetting the kafirin to increase its dielectric properties. Microwave heating this kafirin to 90 or 96°C and holding for 1–2 min more than doubled maximum tensile strength and Young's modulus, and decreased strain by about one‐third compared with films made from nonmicrowaved kafirin. Film water vapor permeability was reduced by at least one‐third. Digestibility of microwaved kafirin and films was also substantially decreased, and film biodegradability was slowed slightly. Microwave heating gave a film microstructure with fewer and smaller size pores. SDS‐PAGE showed microwave‐induced intermolecular cross‐linking of the kafirin monomers, which was possibly responsible for the modification of film properties. Microwave heating of kafirin can be used to modify kafirin film properties, but the kafirin must be microwaved wet and be as close as possible to its native state.     

Estimation of dwarf green bean water use under semi-arid climate conditions through ground-based remote sensing techniques

Determination of temporal and spatial distribution of water use (WU) within agricultural land is critical for irrigation management and could be achieved by remotely sensed data. The aim of this study was to estimate WU of dwarf green beans under excessive and limited irrigation water application conditions through indicators based on remotely sensed data. For this purpose, field experiments were conducted comprising of six different irrigation water levels. Soil water content, climatic parameters, canopy temperature and spectral reflectance were all monitored. Reference evapotranspiration (ET₀), crop coefficient K_c and potential crop evapotraspiration (ET_c) were calculated by means of methods described in FAO-56. In addition, WU values were determined by using soil water balance residual and various indexes were calculated. Water use fraction (WUF), which represents both excessive and limited irrigation applications, was defined through WU, ET₀ and K_c. Based on the relationships between WUF and remotely sensed indexes, WU of each irrigation treatments were then estimated. According to comparisons between estimated and measured WU, in general crop water stress index (CWSI) can be offered for monitoring of irrigated land. At the same time, under water stress, correlation between measured WU and estimated WU based on CWSI was the highest too. However, canopy-air temperature difference (T_c − T_a) is more reliable than others for excessive water use conditions. Where there is no data related to canopy temperature, some of spectral vegetation indexes could be preferable in the estimation of WU.     

Morpho-Physiological Response of Oryza glaberrima to Gradual Soil Drying

Soil drought occurrence during dry season has been the main constraint, besides prolonged flooding during rainy season, in increasing cropping intensity and rice productivity in tropical riparian wetland. Use of drought tolerant rice genotype might be a suitable option for overcoming such problem. This study focused on the effects of gradual soil drying during early vegetative growth stage on morphological and physiological traits of five Oryza glaberrima genotypes, namely RAM12, RAM14, RAM59, RAM97 and RAM101, and two Oryza sativa subsp japonica genotypes, i.e. Koshihikari and Minamihatamochi. The plants were subjected to 6 d of gradual soil drying condition from 15 days after transplanting (DAT) to 20 DAT, and were allowed to recover until 22 DAT. Gradual soil drying reduced plant growth as indicated by dry mass accumulation. Drought reduced stomatal conductance and increased leaf rolling score of all the genotypes. All the genotypes showed comparable response on stomatal conductance, but O. glaberrima genotypes performed higher in leaf rolling recovery. Meanwhile, O. sativa genotypes decreased total leaf area and specific leaf area, but increased specific leaf weight in order to avoid further damages due to drought stress. Drought tolerance mechanisms in RAM101, RAM12, RAM59 and RAM14 were associated with leaf morpho-physiological responses, root traits and dry biomass accumulation.     

Response of selected water chemical quality parameters to slight thinning in a mature oak–beech forest ecosystem under sub-humid climate conditions

The objective of this study was to determine the effect of 18% thinning on streamflow nutrient flux from a mature oak–beech forest ecosystem by paired watershed approach. Two experimental watersheds including control (W-I) and treatment (W-IV) watersheds were used in the study. The experimental watersheds were monitored about 6 years from 2006 to 2011 for the calibration period and 4 years from 2012 to 2015 for the treatment period. The forest in the treatment watershed was thinned between October and December in 2011, and the forest in the control watershed was left untreated. Water grab samples were collected from the streams in the watersheds on weekly basis during both the calibration and treatment periods and analyzed for calcium (Ca²⁺), magnesium (Mg²⁺), Kjeldahl nitrogen (KN), sodium (Na⁺), potassium (K⁺), iron (Fe³⁺), aluminum (Al³⁺), ammonium nitrogen (NH₄ ⁺-N), and sulfate (SO₄ ²⁻). The simple linear regression equations were developed between mean monthly nutrient fluxes of two watersheds in the calibration period with significantly high correlation coefficients, and they were used to estimate nutrient fluxes from the treatment watershed during the treatment period as if thinning had not been applied. The changes in the monthly nutrient fluxes were estimated as the differences between measured and values calculated with the linear regression equations. Results showed that removal of 18% standing timber volume did not significantly change nutrient exports except for KN and Na⁺ from the treatment watershed.

     

Orthogonal cutting mechanics of maple: modeling a solid wood-cutting process

The experimental results of orthogonal cutting of maple and the modeling of the cutting mechanics are presented. The tool cutting forces were measured for different feed rates. A set of equations relating the tangential and feed forces to the tool edge width and feed rate (chip thickness) to calculate the chip and edge cutting force coefficients was developed. Then the chip force and edge force coefficients were calculated from experimentally obtained cutting forces and were plotted in a polar-coordinate system with respect to the fiber orientation of the maple disk. The polar-coordinate presentation of the cutting force results and the calculated cutting force coefficients provides an excellent visual appreciation of the relation between the cutting forces and the wood fiber orientation. Chips were also collected from various sectors of the wood disk. This analysis further identified the effects of fiber orientation and cutting forces on the types of chip formed and hence the cutting mechanics involved. By applying the calculated cutting coefficients for each tool orientation (in respect to the grain) it is possible to predict the feed and tangential forces for any feed rates. There is good agreement between the predicted and measured cutting forces.     

西昆仑山2个海拔位点可培养细菌多样性分析

以西昆仑山2个海拔样点的土样为对象,利用（LB和TSA）2种培养基,基于16S rRNA基因序列的系统发育,采用纯培养法对其可培养细菌多样性进行研究。2样点中共分离到91株细菌,分为25个属,52个种。厚壁菌门（Firmicutes）占总菌数的48.75%,放线菌门（Actinobacteria）占36.25%,γ-变...     

The distribution of important sero-complexes of flaviviruses in Malaysia

Tropical Animal Health and Production - Flaviviruses (FVs) are arthropod-borne viruses of medical and veterinary importance. Numerous species of FVs have been isolated from various host; mainly...     

Genome analysis of methicillin‐resistant Staphylococcus aureus isolated from pigs: Detection of the clonal lineage ST398 in Cameroon and South Africa

Food animals are considered reservoirs of methicillin‐resistant Staphylococcus aureus (MRSA) and are implicated in their zoonotic transmission in the farm‐to‐plate continuum. LA‐MRSA has been reported as a zoonotic agent that has the potential to spread to humans and may cause infections in at‐risk groups. In this study, whole genome sequencing was used to describe the genetic environment (resistance mechanisms, virulence factors and mobile genetic elements) and investigate the genetic lineages of MRSA isolates from pigs in Cameroonian and South African abattoirs. During March–October 2016, 288 nasal and rectal pooled samples from 432 pigs as well as nasal and hand swabs from 82 humans were collected. Genomic DNA was sequenced using an Illumina MiSeq platform. Generated reads were de novo‐assembled using the Qiagen CLC Genomics Workbench and SPAdes. The assembled contigs were annotated, and antibiotic resistance genes, virulence factors, plasmids, SCCmec and phage elements were identified with ResFinder, Virulence Finder, PlasmidFinder, SCCmec Finder and PHAST, respectively. Core genome single nucleotide analysis was undertaken to assess clonal relatedness among isolates. A lower MRSA prevalence was observed in pigs in Cameroon (n = 1/13; 0.07%) compared with South Africa (n = 4/22; 18.18%), and none of the workers were colonized by MRSA. Genome analysis identified various antibiotic resistance genes along with six virulence factors in all isolates. All MRSA isolates belonged to the clonal lineage ST398 (spa‐type t011) and harboured the type Vc SCCmec and several plasmids. Our study shows that the livestock‐associated MRSA clonal lineage ST398 is already present in both Cameroon and South Africa and is probably underestimated in the absence of molecular epidemiological studies. It reveals the serious food safety and public health threat associated with this animal strain and underscores the need for interventions to contain this resistant clone.     

Electricity and water desalination: Separate sites offer value

For the fuel type and water situation in the Middle East, the case is strong for the use of combined cycle technology for power generation and reverse osmosis for potable water production, where each are sited for their maximum economic benefit and interconnected by electric power transmission. Because of the fuel efficiency of Combined Cycle generation technology, its use of liquid/gas fuels and its low need for cooling water, it can be optimized for cost away from cities. Conversely, water desalination by reverse osmosis can be sited in optimal locations to take advantage of its modularity and to minimize water pipeline needs. Electric power transmission provides an inexpensive and flexible means to connect these two technologies. Together these technologies may offer an overall minimum cost approach, better than the combining of electric power and water desalination at one location, where power to water ratios must be fixed, independent of need, for optimum efficiency. The use of reverse osmosis with power generation has other, important ancillary benefits over using distillation and power combinations. These advantages include abatement of environmental pollution, delivery of potable water at reasonable drinking temperatures, lower total energy consumption, more efficient land use and less demanding operator skills.