Freezing behaviors in leaf buds of cold-hardy conifers visualized by NMR microscopy

1H-Nuclear magnetic resonance (NMR) microscopy was used to study freezing behavior in wintering leaf buds of Momi fir (Abies firma Sieb. et Zucc.) and Japanese red pine (Pinus densiflora Sieb. et Zucc.). The images acquired predominantly reflected the density of mobile (i.e., non-ice) protons mainly from unfrozen water. By comparing images taken at various subfreezing temperatures, we determined which tissues produced the high and low temperature exotherms detected by differential thermal analyses. Typical extra-organ freezing was successfully imaged in leaf buds of A. firma. The bud scales readily froze at -7 degrees C, but shoot primordia remained supercooled to -14 degrees C in December buds and to -21 degrees C in March buds. The size of supercooled shoot primordia was reduced with decreasing temperature, indicating a gradual decrease in water content of the shoot primordia. In contrast, the signal from shoot primordia of P. densiflora disappeared between -7 and -14 degrees C, corresponding to the high temperature exotherm at -8 degrees C, indicating extracellular freezing of the shoot primordia. The xylem and bark tissues readily froze at -7 degrees C in A. firma and between -7 and -14 degrees C in P. densiflora. We conclude that NMR microscopy can noninvasively provide more spatially specific information about freezing behavior in leaf buds than traditional methods such as differential thermal analysis. In particular, it allows the organized and harmonized freezing behaviors in complex organs to be visualized directly thereby revealing the diversity of mechanisms involved in freezing behaviors.     

Heterologous gap junctions between granulosa cells and oocytes in ayu (Plecoglossus altivelis): formation and role during luteinizing hormone-dependent acquisition of oocyte maturational competence

Gap junctions (GJs) are intercellular channels. Molecules with a molecular weight of 1 kDa or less can pass back and forth between adjacent cells through GJs. Communication between oocytes and the somatic cells that surround them via GJs is known to play key roles to initiate oocyte maturation in many vertebrates. However, little is known of the detailed functions of ovarian GJs during oocyte maturation in fish. The oocyte maturation of fish is induced by a maturation-inducing steroid (MIS). The sensitivity of oocytes to the MIS is known as oocyte maturational competence (OMC) and is induced by luteinizing hormone (LH). However, LH receptors are found on the surface of granulosa cells rather than oocytes. We therefore proposed that the LH signals received by granulosa cells were passed to oocytes via GJs. This review describes current knowledge of the role of GJs between granulosa cells and oocytes during the LH-induced acquisition of OMC in fish.     

Relationships between the conception rate of estrus synchronization using estradiol benzoate and CIDR (progesterone) and other parameters in holstein lactating dairy cows

The purpose of this study was to determine the relationships between conception rate and other parameters before estrus synchronization with a Controlled Internal Drug Release Device (CIDR) and estradiol benzoate (EB). In the estrus synchronization program, animals were injected with 2 mg EB and then received a CIDR. Seven days later, the CIDR was removed and the animals were given an injection of Prostaglandin F(2alpha). Twenty-four hours later, they received an injection of 1 mg EB, and they were artificially inseminated 24 h after that. This program was applied to 258 Holstein cows in Tohoku-machi (Aomori, Japan). Blood was collected at the beginning of the program, and the conception rate was determined about 40 days after insemination. The relationships among conception rate, blood biochemical values, age, body condition score and days in milk were statistically analyzed to determine better conditions for cow conception. The conception rate of the cows in the high progesterone group (more than 1 ng/ml, P(4)+) was significantly higher than that of the low progesterone group (less than 1 ng/ml, P(4)-; 47.9% vs. 28.6% P<0.01). In the P(4)- groups, the serum phospholipid level was significantly higher in the conception group than in the non-conception group, and the same tendency was seen in the P(4)+ groups. Blood urea nitrogen (BUN), albumin (Alb), and total cholesterol (TChol) were significantly higher in the conception group compared with the non-conception group, but no with P(4) was observed. We concluded that 1) the conception rate of the P(4)- group was remarkably low, that 2) the low conception rate and low P(4) level was related to a low PL level and that 3) BUN, Alb and TChol were higher in the conception group, although no relation with P(4) was found.     

Molecular ordering of organic molten salts triggered by single-walled carbon nanotubes

When mixed with imidazolium ion-based room-temperature ionic liquid, pristine single-walled carbon nanotubes formed gels after being ground. The heavily entangled nanotube bundles were found to untangle within the gel to form much finer bundles. Phase transition and rheological properties suggest that the gels are formed by physical cross-linking of the nanotube bundles, mediated by local molecular ordering of the ionic liquids rather than by entanglement of the nanotubes. The gels were thermally stable and did not shrivel, even under reduced pressure resulting from the nonvolatility of the ionic liquids, but they would readily undergo a gel-to-solid transition on absorbent materials. The use of a polymerizable ionic liquid as the gelling medium allows for the fabrication of a highly electroconductive polymer/nanotube composite material, which showed a substantial enhancement in dynamic hardness.     

Potential conflicts for the reuse of rice husk in Thailand

This study aims to investigate the impact of rice-husk power generation on agricultural uses of rice husk and its ash. We conducted case studies at a rice-husk power plant and organic farming groups in northeast Thailand. The case study on a rice-husk power plant showed that rice husk was useful in generating electricity without consuming fossil fuel, while husk ash, the by-product, could be utilized as silicate supplement for farmland soils. The case study on organic farming groups indicated that rice husk was one of the indispensable materials in conducting organic farming with locally available resources. On the basis of these findings, an analytical study on possible husk uses in Thailand was conducted, in which three potential major uses of rice husk were considered: i.e. (1) fuel use inside rice mills followed by husk-ash reuse, (2) fuel use at power plants followed by husk-ash reuse, and (3) direct reuse of husk for compost production. The result indicated that there would be conflicting demands for rice husk among those uses in the future. Therefore, there needs to be a strategy for balancing husk uses among them.     

Economic and environmental effects of rice-straw bioethanol production in Vietnam

Bioethanol production from rice-straw is carbon neutral and less competitive with food supplies, so it provides great possibilities for resolving global issues. This study aims to conduct economic and environmental evaluations on rice-straw bioethanol production in Vietnam, where huge amounts of unused rice-straw are available, by a top–down life-cycle assessment by means of an input–output table. The economic impacts are evaluated by total costs, total production, and total added value; the environmental impacts are assessed by greenhouse gas emissions with consideration of life-cycle, i.e., the plant construction phase, production phase, and plant scrapping phase. Three technology scenarios are assumed: (1) present technology, (2) advanced technology with higher conversion rates, and (3) innovative technology with a new production method and economies of scale. The results demonstrate that, first, rice-straw bioethanol production can reduce annual gasoline consumption by more than 20 %, and plant construction costs account for 8–22 % of the total investment in Vietnam. Second, under the present technology, both economic and environmental net benefits are negative. However, the innovative technology makes both benefits positive. Third, under the advanced technology, the environmental net benefit is positive, while the economic net benefit is negative. This implies that satisfying economic viability is more difficult than attaining environmental viability in rice-straw bioethanol production. Therefore, technological development and transfer are necessary to make rice-straw bioethanol production feasible.     

Measuring the implicit value of paddy irrigation water: application of RPML model to the contingent choice experiment data in Japan

For efficient use of water as a limited resource, evaluation on the water value is critical, but there is little information in Asian paddy irrigation. This paper proposes the method for measuring implicit price of paddy irrigation water by using the choice experiment (CE) data with contingent scenarios. Empirical results demonstrated that (a) the estimated implicit price of water showed reasonable value as compared to the production indexes, (b) the random parameter multinomial logit model was more suitable than the conventional multinomial logit model to treat the CE data, and (c) the implicit price is much lower than the full cost price, indicating that full cost pricing probably damages and ruins rice production too seriously in Japan. As seen above, the method proposed here is useful for decision making on water pricing policies and easy to apply to different irrigation systems under limited data of water value.

Estimation of terminal molting probability of snow crab <Emphasis Type="Italic">Chionoecetes opilio</Emphasis> using instar- and state-structured model in the waters off the Pacific coast of northern Japan

Terminal molting probability (TMP) of snow crab Chionoecetes opilio was estimated using instar- and state-structured model incorporating the terminal molt in the waters off the Pacific coast of northern Honshu, Japan. TMP is defined as the probability that terminal molt occurs within a year in an instar and is an important parameter for stock assessment. TMP was estimated from the model and the rate of terminal molted crabs (RTM) for instars, which means carapace widths over 30 mm. Carapace widths at RTM of 0.5 were 81 mm for males and 66 mm for females. TMP was estimated as 0.23 and 0.29 for these carapace widths, respectively. Carapace widths at TMP of 0.5 were 94 mm for males and 71 mm for females, and for these carapace widths 57% of male and 50% of female crabs were considered as old-shell which had survived 1 year or more from terminal molt. Changing the fishing or natural mortality by ±0.1, the maximum difference of TMP was less than 0.1 from default estimates.