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991.
SUN Jian YU Jin-feng LUAN Hai-rong LI Shuang XU Xiao-yi HE Zhi-peng WEI Tao LI Li XU Chang-qing 《园艺学报》2014,30(4):651-657
AIM: To observe the functional expression of calcium-sensing receptor (CaSR) in the mouse embryonic stem cells (mESCs). METHODS: The expression and distribution of CaSR were detected by Western blotting and immunofluorescence observation in 129 mouse ES-D3 cells. The intracellular concentration of free calcium ([Ca2+]i) was determined by confocal laser scanning microscopy. The cell viability was analyzed by MTT assay and flow cytometry. RESULTS: CaSR protein was expressed in mESCs. Extracellular calcium or neomycin significantly increased the expression of CaSR and [Ca2+]i. Neomycin increased the cell viability, up-regulated the protein expression of p-ERK2. These effects of neomycin were inhibited by NPS2390. CONCLUSION: CaSR is expressed in mESCs. The activation of CaSR is involved in the proliferation of mESCs. 相似文献
992.
成团泛菌(Pantoe agglomerans)Ljb-2 是从番茄生境中分离得到的对番茄黄化曲叶病毒病
(tomato yellow leaf curl virus disease,TYLCD)具有较好防效的拮抗细菌,其对温室中番茄感病品种‘格
瑞斯’TYLCD 的防控效果为49.77%。2012 年4—8 月在连云港进行的田间试验结果表明,该菌株在田间
条件下对感病品种‘格瑞斯’TYLCD 的防效达到57.12%。2012 年7—12 月,将该菌株进行田间防治试
验,结果表明,番茄移栽60 d 时,Ljb-2 对番茄中抗品种‘迪芬妮’TYLCD 的防效达到45.26%。同时,
用Ljb-2 处理番茄植株后,能显著增加叶片叶绿素a、叶绿素b 及总叶绿素含量,提高果实中维生素C、
可溶性糖、可滴定酸含量及糖酸比,对果实品质具有明显的改善作用。此外,对番茄的增产效果达到
20.05%。 相似文献
993.
CAO Wen-long WEI Wei-yuan ZHANG Xiao-shi LUO Wen YAN Lin-hai XIE Yu-bo XIAO Qiang 《园艺学报》2014,30(4):620-624
AIM: To study the effect and the molecular mechanism of CDX2 over-expression on the proliferation, growth and cell cycle of human gastric cancer cell line SGC-7901. METHODS: The SGC-7901 cells in LV-CDX2-GFP group were transfected with the recombinant lentivirus vector LV-CDX2-GFP, the cells in LV-GFP group were transfected with the negative control lentiviral vector for the negative control, and the cells in blank control group were without any treatment. The cell proliferation was detected by CCK-8 assay. The cell cycle distribution was analyzed by flow cytometry. The expression of CDX2, Bax, Bcl-2, cyclin D1 and survivin was determined by semi-quantitative RT-PCR and Wes-tern blotting. RESULTS: Compared with LV-GFP group and blank control group, the proliferation activity of the SGC-7901 cells was significantly lower (P<0.05), the G0/G1 phase proportion increased (P<0.05), the mRNA and protein levels of Bcl-2, cyclin D1 and survivin were reduced (P<0.05), and the mRNA and protein levels of Bax were up-regulated (P<0.05) in LV-CDX2-GFP group. No statistically significant difference of the above indexes was observed (P>0.05) between LV-GFP group and blank control group. CONCLUSION: Over-expression of CDX2 mediated by lentivirus inhibits the proliferation and growth of human gastric cancer SGC-7901 cells and arrestes the cell cycle at G0/G1 phase, which may be related to down-regulation of Bcl-2, cyclin D1 and survivin and up-regulation of Bax. 相似文献
994.
桂彩薯1 号是以日本北海道岛系571 号为母本,以日本北海道岛系568 号为父本经有性杂交系统选育而成。早中熟,生育期(出苗至成熟)85~95 d(天)。块茎长椭圆形,中等大小,紫皮紫肉,表皮光滑,芽眼少而浅,结薯集中,单薯质量100 g 左右,鲜薯产量1 000~1 500 kg· ( 667 m2)-1,最高可达2 000 kg( 667 m2)-1。块茎淀粉含量9.07%,粗蛋白质2.45%,还原糖0.67%,VC 137.0 mg·kg-1(FW),花色苷1 786.0 mg·kg-1(FW)。适宜广西、广东、福建、云南、湖南等地秋冬种植。 相似文献
995.
996.
LU Na BAI Rui-ying WEI Lin-yu LI Chao-kun LI Xin-juan ZHAO Hong-gang LI Dong-liang 《园艺学报》2014,30(8):1388-1393
AIM:To examine the effects of high concentration of extracellular ATP on human neuroblastoma SH-SY5Y cell injury. METHODS:Cultured SH-SY5Y cells were grouped according to the concentrations of ATP and treatment time. The cell viability was detected by CCK-8 assay. The variation of autophagic vacuoles was observed with monodansylcadaverine staining. The cell apoptosis was analyzed by Hoechst 33258 staining. Meanwhile, apoptotic rate was detected by flow cytometry. The levels of caspase-3 and microtubule-associated protein 1 light chain 3-Ⅱ (LC3-Ⅱ) were determined by Western blotting. RESULTS:Compared with control group, the survival rate of SH-SY5Y cells was significantly reduced by ATP at different concentrations (3, 6, 9, 12 and 15 mmol/L for 3 h) and different treatment time (1, 2, 3 and 6 h with 6 mmol/L ATP, peaking at 3 h). The autophagic vacuoles of SH-SY5Y cells were significantly increased at 1 h with ATP treatment, trended to decrease over time and returned to control level at 6 h. The protein expression of LC3-Ⅱ was significantly increased at 1 h with ATP treatment, which was consistent with the time points of increasing autophagic vacuoles. LC3-Ⅱ expression level gradually decreased at 2~3 h with ATP treatment, and returned to control level at 6 h. Compared with control group, the apoptotic rate and the expression level of caspase-3 were enhanced synchronously. The peak of apoptotic rate occurred at 3 h, and kept until 6 h.The level of cleaved caspase-3 expression peaked at 6 h. CONCLUSION:High concentration of extracellular ATP induces the autophagy and apoptosis of SH-SY5Y cells. The increased autophagy shows up, followed by the climax of apoptosis until 6 h. With the prolonged duration of ATP, apoptosis is the main process in the cells. 相似文献
997.
以鸡毛菜为材料,通过控制穴盘质量,研究不同灌水量(100%ET、90%ET、80%ET和70%ET)对鸡毛菜生长和生理的影响。试验结果表明,补充灌水量100%ET及90%ET处理下鸡毛菜地上部干鲜质量、株高、茎粗均最高;80%ET处理下鸡毛菜叶片叶绿素含量、可溶性蛋白质含量、可溶性糖含量最优,但该处理下鸡毛菜生物量显著低于100%ET和90%ET处理的;70%ET处理除鸡毛菜可溶性蛋白质外,生物量与品质均显著低于其他处理。但100%ET处理耗水量高于90%ET处理的,因此,综合鸡毛菜生物量、品质以及耗水量,在试验设计的范围内90%ET灌水量是最适合鸡毛菜生长的灌水处理。 相似文献
998.
用辣椒耐热自交系A11和热敏自交系B22配置杂交组合,通过高温胁迫处理鉴定其F2代分离群体的耐热性。从123对Taq I/Ase I引物组合中筛选出6对多态性好的引物对F2代耐热、热敏植株进行AFLP分析,共扩增出约11 400条清晰条带,其中2条为稳定的差异,初步获得了2个与辣椒耐热性状相关的AFLP分子标记。研究有助于开展辣椒耐热基因的分子标记辅助选择工作,提高辣椒育种效率和水平,并为分离和克隆辣椒耐热相关功能基因奠定基础。 相似文献
999.
1000.