全文获取类型
收费全文 | 1483篇 |
免费 | 55篇 |
国内免费 | 4篇 |
专业分类
林业 | 134篇 |
农学 | 34篇 |
基础科学 | 1篇 |
185篇 | |
综合类 | 71篇 |
农作物 | 52篇 |
水产渔业 | 219篇 |
畜牧兽医 | 738篇 |
园艺 | 17篇 |
植物保护 | 91篇 |
出版年
2023年 | 7篇 |
2022年 | 7篇 |
2021年 | 46篇 |
2020年 | 28篇 |
2019年 | 33篇 |
2018年 | 34篇 |
2017年 | 31篇 |
2016年 | 47篇 |
2015年 | 39篇 |
2014年 | 55篇 |
2013年 | 82篇 |
2012年 | 68篇 |
2011年 | 93篇 |
2010年 | 42篇 |
2009年 | 52篇 |
2008年 | 85篇 |
2007年 | 78篇 |
2006年 | 70篇 |
2005年 | 67篇 |
2004年 | 65篇 |
2003年 | 66篇 |
2002年 | 53篇 |
2001年 | 69篇 |
2000年 | 53篇 |
1999年 | 35篇 |
1998年 | 11篇 |
1997年 | 4篇 |
1996年 | 10篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 22篇 |
1991年 | 18篇 |
1990年 | 22篇 |
1989年 | 20篇 |
1988年 | 10篇 |
1987年 | 18篇 |
1986年 | 11篇 |
1985年 | 14篇 |
1984年 | 8篇 |
1983年 | 5篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 8篇 |
1975年 | 7篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1971年 | 4篇 |
1970年 | 4篇 |
1965年 | 4篇 |
排序方式: 共有1542条查询结果,搜索用时 15 毫秒
991.
Ogawa M Yoshimori T Suzuki T Sagara H Mizushima N Sasakawa C 《Science (New York, N.Y.)》2005,307(5710):727-731
The degradation of undesirable cellular components or organelles, including invading microbes, by autophagy is crucial for cell survival. Here, Shigella, an invasive bacteria, was found to be able to escape autophagy by secreting IcsB by means of the type III secretion system. Mutant bacteria lacking IcsB were trapped by autophagy during multiplication within the host cells. IcsB did not directly inhibit autophagy. Rather, Shigella VirG, a protein required for intracellular actin-based motility, induced autophagy by binding to the autophagy protein, Atg5. In nonmutant Shigella, this binding is competitively inhibited by IcsB binding to VirG. 相似文献
992.
Antisense transcription in the mammalian transcriptome 总被引:2,自引:0,他引:2
Katayama S Tomaru Y Kasukawa T Waki K Nakanishi M Nakamura M Nishida H Yap CC Suzuki M Kawai J Suzuki H Carninci P Hayashizaki Y Wells C Frith M Ravasi T Pang KC Hallinan J Mattick J Hume DA Lipovich L Batalov S Engström PG Mizuno Y Faghihi MA Sandelin A Chalk AM Mottagui-Tabar S Liang Z Lenhard B Wahlestedt C;RIKEN Genome Exploration Research Group;Genome Science Group 《Science (New York, N.Y.)》2005,309(5740):1564-1566
993.
994.
Tsuchiyama A Uesugi M Matsushima T Michikami T Kadono T Nakamura T Uesugi K Nakano T Sandford SA Noguchi R Matsumoto T Matsuno J Nagano T Imai Y Takeuchi A Suzuki Y Ogami T Katagiri J Ebihara M Ireland TR Kitajima F Nagao K Naraoka H Noguchi T Okazaki R Yurimoto H Zolensky ME Mukai T Abe M Yada T Fujimura A Yoshikawa M Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1125-1128
Regolith particles on the asteroid Itokawa were recovered by the Hayabusa mission. Their three-dimensional (3D) structure and other properties, revealed by x-ray microtomography, provide information on regolith formation. Modal abundances of minerals, bulk density (3.4 grams per cubic centimeter), and the 3D textures indicate that the particles represent a mixture of equilibrated and less-equilibrated LL chondrite materials. Evidence for melting was not seen on any of the particles. Some particles have rounded edges. Overall, the particles' size and shape are different from those seen in particles from the lunar regolith. These features suggest that meteoroid impacts on the asteroid surface primarily form much of the regolith particle, and that seismic-induced grain motion in the smooth terrain abrades them over time. 相似文献
995.
Chie Suzuki Koji Yoshioka Manabu Yamada Toru Miyamoto Noboru Manabe 《Veterinary research communications》2014,38(1):1-10
We examined the gene and protein levels of tumor necrosis factor (TNF)-α, its receptors (types I and II, designated TNF-RI and TNF-RII, respectively), TNF receptor-associated factor 2 (TRAF2) and morphological features in the porcine corpus luteum (CL), on Days 13 and 17 (Day 0 = the last day of estrus) of the estrous cycle or of early pregnancy. Gene expression levels of TNF-α, TNF-RI, TNF-RII and TRAF2 were unaffected by the day or reproductive status. TNF-α concentration was significantly higher in the CL on Day 17 of pregnancy than on Day 13 of pregnancy and on day 17 of the estrous cycle. The TNF-RI protein level was significantly higher in the CL on Days 13 and 17 of pregnancy than those of the estrous cycle, significantly increasing on Day 17 compared with those on Day 13 in pregnancy. In relation to TNF-RII protein levels, although there were no change during pregnancy, there was a tendency (P?=?0.0524) to up-regulate as pregnancy proceeded. In estrous cycle, TNF-RII protein levels decreased significantly as luteolysis proceeded. TRAF2 protein level was significantly higher in the CL on Days 13 and 17 of pregnancy than during estrous. There were few apoptotic bodies in the CL between Days 13 and 17 of pregnancy than during esrous. There were few apoptotic bodies in the CL between Days 13 and 17 of pregnancy. The number of apoptotic bodies was much greater than the CL on Day 17 of the estrous than those of pregnancy. Thus, the TNF-α and TNF-RI and TNF-RII pathways including the TRAF2 protein, known to control of cell differentiation, tissue renewal and apoptosis, might participate in maintaining the porcine CL during early pregnancy. 相似文献
996.
Muneaki Takahata Hidehiro Toh Akiyo Nakano Misako Takagi Masaru Murakami Yasuo Ishii Tatsuya Takizawa Soichi Tanabe Hidetoshi Morita 《Animal Science Journal》2014,85(2):158-163
Bifidobacterial plasmids reported so far are derived from a limited number of strains and plasmids of bifidobacterial type strains isolated from humans are unknown. We found that Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from a healthy infant contained two cryptic plasmids, designated pBBKW‐1 and pBBKW‐2. We determined and analyzed the complete sequences of both plasmids. pBBKW‐1 (7716 bp) was predicted to replicate by a rolling‐circle mechanism and encode six protein‐coding genes, two of which are putative replication proteins. pBBKW‐1 seems to be a cointegrate plasmid containing two copies of the plasmid pMG1 from Bifidobacterium longum. pBBKW‐2 (2920 bp) was predicted to encode six protein‐coding genes and be a theta‐type replicating plasmid, which has been reported to be more stable than a rolling circle‐type replicating plasmid frequently found in bifidobacteria. Our finding will provide new insights into safe recombinant plasmid constructions for humans. 相似文献
997.
The present study sought to assess the potential application of avian models with different developmental modes to studies on cognition and neuroscience. Six altricial Bengalese finches (Lonchura striata var. domestica), and eight precocial blue‐breasted quails (Coturnix chinensis) were presented with color discrimination tasks to compare their respective faculties for learning and memory retention within the context of the two developmental modes. Tasks consisted of presenting birds with discriminative cues in the form of colored feeder lids, and birds were considered to have learned a task when 80% of their attempts at selecting the correctly colored lid in two consecutive blocks of 10 trials were successful. All of the finches successfully performed the required experimental tasks, whereas only half of the quails were able to execute the same tasks. In the learning test, finches required significantly fewer trials than quails to learn the task (finches: 13.5 ± 9.14 trials, quails: 45.8 ± 4.35 trials, P < 0.05), with finches scoring significantly more correct responses than quails (finches: 98.3 ± 4.08%, quails: 85.0 ± 5.77% at the peak of the learning curve). In the memory retention tests, which were conducted 45 days after the learning test, finches retained the ability to discriminate between colors correctly (95.0 ± 4.47%), whereas quails did not retain any memory of the experimental procedure and so could not be tested. These results suggested that altricial and precocial birds both possess the faculty for learning and retaining discrimination‐type tasks, but that altricial birds perform better than precocial birds in both faculties. The present findings imply that developmental mode is an important consideration for assessing the suitability of bird species for particular experiments. 相似文献
998.
Satoshi Yamamoto Kazuhiko Ito Kii Suzuki Yuki Matsushima Izumi Watanabe Yutaka Watanabe Keima Abiko Toshihiko Kamada Kan Sato 《Animal Science Journal》2014,85(3):330-335
This study investigated how supplementation of the diet of dairy cows with trace minerals (zinc, manganese, copper and cobalt) affected kinematic gait parameters and lactation performance. Eight Holstein cows were divided into two groups, with each group receiving a different dietary treatment (control diet, or control diet supplemented with trace minerals) in a two‐period crossover design. Kinematic gait parameters were calculated by using image analysis software. Compared to cows fed the control diet, cows that received the trace mineral‐supplemented diet exhibited significantly increased walking and stepping rates, and had a shorter stance duration. Feed intake and milk production increased in cows fed the trace mineral‐supplemented diet compared with control groups. The plasma manganese concentration was not different in control and experimental cows. In contrast, cobalt was only detected in the plasma of cows fed the supplemented diet. These results provide the first evidence that trace mineral supplementation of the diet of dairy cows affects locomotion, and that the associated gait changes can be detected by using kinematic gait analysis. Moreover, trace mineral supplementation improved milk production and only minimally altered blood and physiological parameters in dairy cows. 相似文献
999.
Effects of modern drainage systems on the spatial distribution of loach in rice ecosystems 总被引:1,自引:0,他引:1
- 1. Although rice fields support many aquatic species, recent agricultural intensification in Asia has led to loss of biodiversity, such as freshwater fish, in exchange for increased agricultural productivity. In Japan, modern drainage systems have been introduced in recent decades.
- 2. This study investigated the effects of several aspects of modern drainage systems on the spatial distribution of dojo loach Misgurnus anguillicaudatus, a dominant fish species in Japanese rice fields.
- 3. Multimodel inference approaches and hierarchical partitioning were used to estimate the direction and degree of the effect of each system on the spatial distribution of dojo loach.
- 4. Three variables related to modern drainage systems (gaps between fields and ditches, the type of drainage ditch, and the presence of underground drainage pipes), flooding dates, and spatial autocovariates showed significant influence on the abundance of loach. In particular, gaps had the largest impact among all the variables on loach in the later part of the survey (June).
- 5. Based on the results, the effectiveness of several conservation methods implemented for paddy‐dwelling fish in Japan was discussed. The knowledge will be a guide to the appropriate conservation of freshwater fish in Asia and other countries.
1000.
J.K. Sundaray K. Ohta A. Yamaguchi K. Suzuki M. Matsuyama 《Fish physiology and biochemistry》2003,28(1-4):193-195
The wrasse, Pseudolabrus sieboldi, is a diandric protogynous labrid fish. Spawning is performed by a terminal phase (TP) male and an initial phase (IP) female between 6:00 and 9:00 h daily during two-month-long spawning season. In the present study, to investigate the roles of steroid hormones in the diurnal spermatogenesis of the P. sieboldi TP male, all steroid hormones produced in the testis were identified and the synthetic pathways of these steroids were determined. Furthermore, the circulating levels of the major steroids produced were analyzed throughout a day at 3-hour intervals during spawning season. In the testis, 11-ketotestosterone (11-KT), estradiol-17β (E2), 17,20β-dihydoxy-4-pregnane-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) were synthesized as the major metabolites. In vitro steroid biosynthesis experiments showed similar results to the circulation profiles of the major steroids. This study is the first to clarify the complete steroidogenic pathways in the gonads of a diandric protogynous species throughout its life, when combined with the results of the steroidogenesis in the ovarian follicles. This is also the first report of a clear diurnal rhythm of the steroid production corresponding to the spermatogenic process in the testis of a male teleost. 相似文献