Long-term continuous cropping in the Pacific Northwest: Tillage and fertilizer effects on winter wheat, spring wheat, and spring barley production

Conventional tillage winter wheat (Triticum aestivum) (WW)–summer fallow reduces soil productivity and increases soil erosion. Conservation tillage management, together with intensive cropping may have the potential to reverse these sustainability concerns. The objective of this study was to determine the effects of conventional tillage (CT) and no-tillage (NT) systems on grain yield of long-term annual cropping of monoculture WW, spring wheat (SW), and spring barley (Hordeum vulgare) (SB) grown with or without fertilizer, in the Pacific Northwest region of the USA. In unfertilized crops, grain yield of WW, SW, and SB was 15%, 25%, and 50% higher, respectively, in CT than in NT plots, an indication of the involvement of yield limiting factors under the NT cropping system. When fertilized, there were no significant differences in grain yield of WW. Yields of SW and SB, however, remained 21% and 15% higher, respectively, in CT than in NT, an indication that factors other than fertility were involved. These results suggest that in order for NT management to be widely adopted by area growers, the yield-limiting factors need to be addressed.     

Evaluating estimators of the numbers of females with cubs-of-the-year in the yellowstone grizzly bear population

Current management of the grizzly bear (Ursus arctos) population in Yellowstone National Park and surrounding areas requires annual estimation of the number of adult female bears with cubs-of-the-year. We examined the performance of nine estimators of population size via simulation. Data were simulated using two methods for different combinations of population size, sample size, and coefficient of variation of individual sighting probabilities. We show that the coefficient of variation does not, by itself, adequately describe the effects of capture heterogeneity, because two different distributions of capture probabilities can have the same coefficient of variation. All estimators produced biased estimates of population size with bias decreasing as effort in creased. Based on the simulation results we recommend the Chao estimator for model M _h be used to estimate the number of the female bears with cubs of the year; however, the estimator of Chao and Shen may also be useful depending on the goals of the research.     

An Assessment of Mercury in the Form of Amalgam in Dental Wastewater in the United States

An assessment was conducted of the discharge from dental facilities of mercury in the form of amalgam to surface waters in the United States. Two pathways were examined – effluent from publicly owned treatment works (POTWs) and air emissions from sewage sludge incinerators (SSIs). The annual use of mercury in the form of amalgam in the U.S. is approximately 35.2 tons (31.9 metric tons). It was estimated that 29.7 tons (26.9 metric tons) of mercury in the form of amalgam are annually discharged to the internal wastewater systems of dental facilities during amalgam placements and removals. Based on the partial capture of this amalgam in conventional chair-side traps and vacuum filters, the discharge of mercury in the form of amalgam from dental facilities to POTWs was estimated to be 6.5 tons (5.9 metric tons). The discharge of mercury to surface water via POTW effluents and SSI emissions was estimated to total approximately 0.4 tons (0.4 metric tons). A cost-effectiveness analysis determined that the annual cost to the dental industry to reduce mercury discharges through the use of amalgam separators would range from 380 millionto 1.14 billion per ton.     

Changes in light attenuation during filling,stabilization and operation of a reservoir

Water, Air, &; Soil Pollution - Transformations in light attenuation (Kd) and Secchi disc transparency (SD) in Round Valley Reservoir, New Jersey, are documented over a 17 yr period...     

Development and Implementation of a Forest Nursery Accreditation Policy at a Local Level in Leyte, Philippines

Through a series of workshops with the stakeholders including nursery operators, tree farmers, staff of the Department of Environment and Natural Resources, local government units and the academe, a forestry seedling nursery accreditation policy was developed. The resulting policy was subsequently implemented by the municipal government of Palompon, Leyte. The accreditation policy encouraged nursery operators to follow smallholder-based best management practices for tree nurseries. Implementation of the nursery accreditation policy improved farmers’ knowledge and skills in the production of high quality seedlings, encouraged adoption of BMP for forest nurseries, provided additional source of income for the farmers, enabled members of the groups operating the communal nursery to forge closer relationship with each other, encouraged utilization of farmers’ free time in productive activities, developed farmers’ confidence in producing high quality planting materials, and gained for the farmers favourable attention from various organizations and groups. The Bennett’s hierarchy of project outcomes showed that the implementation of accreditation scheme achieved high levels of outcomes, indicating that it led to substantial improvements in the lives of the farmers.     

Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures

Snake presynaptic phospholipase A2 neurotoxins (SPANs) paralyze the neuromuscular junction (NMJ). Upon intoxication, the NMJ enlarges and has a reduced content of synaptic vesicles, and primary neuronal cultures show synaptic swelling with surface exposure of the lumenal domain of the synaptic vesicle protein synaptotagmin I. Concomitantly, these neurotoxins induce exocytosis of neurotransmitters. We found that an equimolar mixture of lysophospholipids and fatty acids closely mimics all of the biological effects of SPANs. These results draw attention to the possible role of local lipid changes in synaptic vesicle release and provide new tools for the study of exocytosis.     

Mutational analysis of the tyrosine phosphatome in colorectal cancers

Tyrosine phosphorylation, regulated by protein tyrosine phosphatases (PTPs) and kinases (PTKs), is important in signaling pathways underlying tumorigenesis. A mutational analysis of the tyrosine phosphatase gene superfamily in human cancers identified 83 somatic mutations in six PTPs (PTPRF, PTPRG, PTPRT, PTPN3, PTPN13, PTPN14), affecting 26% of colorectal cancers and a smaller fraction of lung, breast, and gastric cancers. Fifteen mutations were nonsense, frameshift, or splice-site alterations predicted to result in truncated proteins lacking phosphatase activity. Five missense mutations in the most commonly altered PTP (PTPRT) were biochemically examined and found to reduce phosphatase activity. Expression of wild-type but not a mutant PTPRT in human cancer cells inhibited cell growth. These observations suggest that the mutated tyrosine phosphatases are tumor suppressor genes, regulating cellular pathways that may be amenable to therapeutic intervention.     

The cell and molecular basis of mechanical, cold, and inflammatory pain

Peripheral pain pathways are activated by a range of stimuli. We used diphtheria toxin to kill all mouse postmitotic sensory neurons expressing the sodium channel Nav1.8. Mice showed normal motor activity and low-threshold mechanical and acute noxious heat responses but did not respond to noxious mechanical pressure or cold. They also showed a loss of enhanced pain responses and spontaneous pain behavior upon treatment with inflammatory insults. In contrast, nerve injury led to heightened pain sensitivity to thermal and mechanical stimuli indistinguishable from that seen with normal littermates. Pain behavior correlates well with central input from sensory neurons measured electrophysiologically in vivo. These data demonstrate that Na(v)1.8-expressing neurons are essential for mechanical, cold, and inflammatory pain but not for neuropathic pain or heat sensing.