Potential nitrogen immobilization as influenced by available carbon in Japanese arable and forest soils

Abstract

Iron oxide is the most important electron acceptor in paddy fields. We aimed to suppress the methane emission from paddy fields over the long term by single application of iron materials. A revolving furnace slag (RFS; 245 g Fe kg^-1) and a spent disposable portable body warmer (PBW; 550 g Fe kg_-1) were used as iron materials. Samples of a soil with a low iron level (18.5 g Fe kg^-1), hearafter referred to as “a low-iron soil” and of a soil with a high iron level (28.5 g Fe kg^-1), hearafter referred to as “an iron-rich soil,” were put into 3 L pots. At the beginning of the experiment, RFS was applied to the pots at the rate of 20 and 40 t ha^-1, while PBW was applied at the rate of 10 t ha^-1 only, and in the control both were not applied. Methane and nitrous oxide emissions from the potted soils with rice plants were measured by the closed chamber method in 2001 and 2002. When RFS was applied at the rates of 20 and 40 t ha^-1 to the low-iron soil, the total methane emission during the cultivation period significantly decreased by 25–50% without a loss of grain yield. Applied iron materials clearly acted as electron acceptors, based on the increase in the amount of ferrous iron in soil. However, the suppressive effect was not evident in the iron-rich soil treated with RFS or PBW. On the other hand, nitrous oxide emission increased by 30–95%. As a whole, when the total methane and nitrous oxide emissions in the low-iron soil were converted to total greenhouse gas emissions expressed as CO₂- C equivalents in line with the global warming potential, the total greenhouse gas emissions decreased by about 50% due to the application of RFS.     

Spatiotemporal variability in soil salinity and its effects on rice (Oryza sativa L.) production in the north central coastal region of Vietnam

Abstract

To better understand the adverse impacts of soil salinization and promote rice (Oryza sativa L.) production in crops from the north central coastal region of Vietnam, the spatiotemporal variability of soil salinity and its effects on rice production were investigated. Experiments were conducted at 19 plots widely distributed in the Quang Phuoc commune, in the Quang Dien district of the province of Thua Thien Hue in the north central coastal region of Vietnam. We determined the elevation of the 19 plots to evaluate the influence of elevation on salt accumulation. Soil samples from the 19 plots were collected in January, May and September 2012 and 2013 to study the spatiotemporal variability of salinity. A soil saturation paste was prepared and used to measure electrical conductivity (ECe). The elevation measurements obtained suggest that the research site could be divided into low- and high-elevation plots, with elevation of the low-elevation plots ranging from –0.52 to 0.07 m and the high-elevation plots from 0.26 to 0.86 m (one of the sampling plot was designated with an elevation of 0 m and the elevations of the remaining 18 plots were measured relative to that). ECe was high at low elevations. In conclusion, although the differences in elevation between the 19 plots were very small (centimeters to decimeters), they still led to large differences in soil salinity levels. In the high-elevation plots, soils were irrigated with freshwater, thus maintaining low ECe levels throughout the year (< 1.0 dS m^?1). In contrast, in the low-elevation plots, soils were subject to seawater intrusion, resulting in high ECe levels in all seasons (> 1.9 dS m^?1). We recommend several solutions to limit the unfavorable effects of salinity and promote rice production. First, a comprehensive dike system should be constructed along the lagoon to prevent seawater intrusion onto land. Second, it will be necessary to construct adequate drainage facilities in the depressed areas to promote rapid water drainage into canals during and after flooding and irrigation. Third, because ECe was high from May to September, adequate fresh irrigation water should be frequently supplied to lower the ECe during this period.     

Interactive effects of in situ rainwater harvesting techniques and fertilizer sources on mitigation of soil moisture stress for sorghum (Sorghum bicolor (L.) Moench) in dryland areas of Tanzania

Nutrient deficiency, high rate of evapotranspiration, and insufficient and erratic rainfall are the critical challenges for crop production in the dryland areas (DLAs) of Sub-Saharan Africa, including Tanzania, where 61% of arable land is prone to drought. In addressing these challenges, field trials were conducted in central Tanzania to evaluate the interactive effects of ripping and tie-ridges with organic (FYM) and inorganic fertilizers (N) on the mitigation of the critical period of soil moisture stress (CPSMS) for sorghum yield performance. Both in situ rainwater harvesting techniques (IRWHT) and flat-cultivated land were integrated with 8 Mg FYM ha^–1, 70 Kg N ha^–1, and a combination of 35 Kg N ha^–1 and 4 Mg FYM ha^–1 (N+ FYM). Among the IRWHT, tie-ridges stored a significant water volume of 577 and 457 m³ ha^–1, which mitigated the CPSMS by the maximum of 95% and 37% for the above-average rainfall and below-average rainfall season, respectively. However, it only registered the highest grain yield (2.02 Mg ha^–1) and biomass (3.46 Mg ha^–1) in a below-average rainfall season. The highest overall grain yield (5.73 Mg ha^–) and biomass (12.09 Mg ha^–1) were harvested in ripping with combined fertilizer treatments in an above-average rainfall season, while the lowest grain yield (0.5 Mg ha^–1) and biomass (1.2 Mg ha^–1) were registered in the flat-cultivation control in the below-average rainfall season. In the latter season, IRWHT increased the mitigation potential in the order; flat cultivation < ripping < tie-ridges; and sorghum yield, highly correlated with drought mitigation index. The results showed that sorghum grain yield and final biomass performance depend on the influence of IRWHT applied, rainfall amount, soil moisture level, integrated fertilizer, and length of the CPSMS. In the above-average rainfall seasons, fertilizers mask the influence of the IRWHT. The opposite is true in the below-average rainfall season. Although ripping_– N+ FYM resulted in the highest overall yield, the study recommends practicing tie-ridges integrated with N+ FYM due to regular occurrences of low and unreliable rainfall in the dryland areas.     

ULTRASONOGRAPHIC FINDINGS RELATED TO PROGNOSIS IN CANINE TRANSITIONAL CELL CARCINOMA

In human bladder cancer patients, ultrasonography is extensively used not only to identify tumor masses but also to evaluate tumor size, shape, echogenicity, location, and degree of tumor invasion into the bladder wall. The information revealed by ultrasonography delineates the tumor's biological features and facilitates prediction of prognosis. However, in veterinary medicine the feasibility of using ultrasonography for these purposes has not been fully investigated. In this retrospective study, we reviewed cases of dogs with histologically confirmed bladder mass lesions, including transitional cell carcinoma (n = 22) and polypoid cystitis (n = 5), to determine whether ultrasonography could reliably predict bladder wall involvement. By following patients with transitional cell carcinoma until death, we also determined whether ultrasonographic tumor size, shape, echogenicity, and mass location were related to prognosis. Wall involvement as revealed by ultrasound was significantly (P = 0.00005) associated with histological muscular layer involvement with a sensitivity of 93% (95% Confidence interval, 79–98%) and specificity of 92% (95% Confidence interval, 76–98%). Ultrasonographic wall involvement (P = 0.03, vs. noninvolvement), heterogeneous mass (P = 0.02, vs. homogeneous mass), and trigone location (P = 0.01, vs. other locations) characteristics were significantly associated with shorter survival times in transitional cell carcinoma cases. Findings indicated that ultrasonographic characteristics such as wall involvement, heterogeneous mass, and trigone location could be reliable prognostic indicators in canine transitional cell carcinoma.     

Effects of Full-Length Kisspeptin Administration on Follicular Development in Japanese Black Beef Cows

Kisspeptin is a key molecule that stimulates gonadotropin secretion via release of gonadotropin-releasing hormone (GnRH). In the present study, our aim was to investigate whether kisspeptin has stimulatory effects on follicular development via GnRH/gonadotropin secretion in cows. Japanese Black beef cows were intravenously injected with full-length bovine kisspeptin [Kp-53 (0.2 or 2 nmol/kg)] or vehicle 5 days after they exhibited standing estrus (Day 0). In cows injected with Kp-53 at 2 nmol/kg, the follicular sizes of the first dominant follicles increased on Day 6 and thereafter. Ovulation of the first dominant follicle occurred in 1 out of 4 cows treated with Kp-53 at 2 nmol/kg. Injection of Kp-53 at 2 nmol/kg increased the concentration of plasma luteinizing hormone (LH) but not follicle-stimulating hormone, over a 4-h period following injection in all cows. The present study suggests that administration of full-length kisspeptin causes LH secretion, which is sustained for a few hours, and it is capable of stimulating follicular development and/or ovulation.     

Indigenous genetic lineages of Fagus crenata found in the Izu Peninsula suggest that there was one of refugia for the species during the last glacial maximum

Regions believed to be refugia are known to have lineages indigenous to the regions. The Izu Peninsula, where genus Fagus existed during the last glacial period, was focused on to survey chloroplast DNA of Japanese beech (Fagus crenata Blume) that grew there. DNA sequence analysis of chloroplast genome regions of about 14 kb revealed two new haplotypes, O and E-1, in F. crenata populations in the Izu Peninsula and adjacent regions. Both haplotypes were conceivably derived from haplotype E. The result of the genetic analysis in this study in combination with the result of pollen analyses performed in the past clearly showed that refugia for F. crenata existed in the Izu Peninsula, and that F. crenata now in growth are lineages indigenous to the region.     

Conversion of abandoned paddy fields to productive land through mangrove restoration in Myanmar’s Ayeyarwady Delta

Paddy and Water Environment - In Myanmar’s Ayeyarwady Delta, mangrove forests were widely converted to paddy fields, then abandoned. However, the mangrove ecosystems often fail to recover. To...     

Identification of tamaraw (Bubalus mindorensis) from natural habitat‐derived fecal samples by PCR‐RFLP analysis of cytochrome b gene

Fecal DNA analysis is a useful tool for the investigation of endangered species. Tamaraw (Bubalus mindorensis) is endemic to the Philippine island of Mindoro but knowledge of its genetic and ecological information is limited. In this study, we developed a species identification method for tamaraw by fecal DNA analysis. Eighteen feces presumed to be from tamaraw were collected in Mount Iglit‐Baco National Park and species‐known feces from domestic buffaloes and cattle were obtained from a farm. Additionally, one species‐unknown fecal sample was obtained in Mount Aruyan Preserve, where the sighting of tamaraw has not been reported in recent years. Based on DNA sequence data previously reported, the genus Bubalus‐ and tamaraw‐specific primers for PCR of cytochrome b gene were newly designed. The Bubalus‐specific primer yielded a 976 bp fragment of cytochrome b for all fecal samples from tamaraw and domestic buffaloes, but not for cattle, whereas the tamaraw‐specific primer yielded a 582 bp fragment for all tamaraw fecal samples and for one of the four domestic buffalo samples. PCR‐RFLP (restriction fragment length polymorphism) analysis of the 976 bp PCR fragment with AvrII or BsaXI provided distinct differences between tamaraw and domestic buffalo. PCR‐RFLP analysis also showed that the species‐unknown sample obtained in Mount Aruyan Preserve, originates from tamaraw.