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391.
Feasibility of supercritical carbon dioxide as a carrier solvent for preservative treatment of wood-based composites 总被引:4,自引:0,他引:4
Supercritical carbon dioxide (SC-CO2) was tested for its potential as a carrier solvent for preservative treatment of solid wood and wood-based composites. A
preliminary trial showed that the treatability of solid wood varied with its original permeability and that the SC-CO2 treatment was not promising for refractory timber species such a Larix leptolepis Gordon. In contrast, 3-iodo-2-propynyl butylcarbamate (IPBC)/SC-CO2 treatment resulted in enhanced decay resistance without any detrimental physical or cosmetic damage in all structural-use
wood-based composites tested: medium density fiberboard, hardwood plywood, softwood plywood, particleboard, and oriented strand
board (OSB). Further trials under various treatment conditions [25°C/7.85 MPa (80 kgf/cm2), 35°C/7.85 MPa, 45°C/7.85 MPa, 35°C/11.77 MPa (120 kgf/cm2), and 45°C/11.77 MPa] indicated that although small changes in the weight and thickness of the treated materials were noted
the strength properties were not adversely affected, except for a few cases of softwood plywood and oriented strand board.
The results of this study clearly indicated that the treatment condition allowed SC-CO2 to transport IPBC into wood-based composites, and the optimum treatment condition seemed to vary with the type of wood-based
composite.
Received: October 24, 2001 / Accepted: February 15, 2002
Part of this work was presented at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001; and the
32nd Annual Meeting of the International Research Group on Wood Preservation, Nara, May 2001
Correspondence to:M. Muin 相似文献
392.
Required sample size for estimating soil respiration rates in large areas of two tropical forests and of two types of plantation in Malaysia 总被引:1,自引:0,他引:1
Minako Adachi Yukiko Sakata Bekku Akihiro Konuma Wan Rasidah Kadir Toshinori Okuda Hiroshi Koizumi 《Forest Ecology and Management》2005,210(1-3):455-459
We estimated the required sample sizes for estimating large-scale soil respiration (for areas from 1 to 2 ha) in four ecosystems (primary and secondary forests, and oil palm and rubber plantations) in Malaysia. The soil respiration rates were 769 ± 329 mg CO2 m−2 h−1 in the primary forest (2 ha, 50 sample points), 708 ± 300 mg CO2 m−2 h−1 in the secondary forest (2 ha, 50 points), 815 ± 363 mg CO2 m−2 h−1 in the oil palm plantation (1 ha, 25 points), and 450 ± 178 mg CO2 m−2 h−1 in the rubber plantation (1 ha, 25 points). According to our sample size analysis, the number of measurement points required to determine the mean soil respiration rate at each site with an error in the mean of no more than 10% ranged from 67 to 85 at the 95% probability level. These results suggest that evaluating the spatial heterogeneity of soil respiration rates in the tropics may require more measurement points than in temperate forests. 相似文献
393.
Takeshi Watanabe Atsuhiko Kato Hiromichi Terashima Koichi Matsubara Yu Jau Chen Kenji Adachi Hideaki Mizuno Masami Suzuki 《Journal of toxicologic pathology》2015,28(1):43-49
Recently, large-scale gene expression profiling is often performed using RNA extracted from unfixed frozen or formalin-fixed paraffin embedded (FFPE) samples. However, both types of samples have drawbacks in terms of the morphological preservation and RNA quality. In the present study, we investigated 30 human prostate tissues using the PFA-AMeX method (fixation using paraformaldehyde (PFA) followed by embedding in paraffin by AMeX) with a DNA microarray combined with laser-capture microdissection. Morphologically, in contrast to the case of atypical adenomatous hyperplasia, loss of basal cells in prostate adenocarcinomas was as obvious in PFA-AMeX samples as in FFPE samples. As for quality, the loss of rRNA peaks 18S and 28S on the capillary electropherograms from both FFPE and PFA-AMeX samples showed that the RNA was degraded equally during processing. However, qRT-PCR with 3’ and 5’ primer sets designed against human beta-actin revealed that, although RNA degradation occurred in both methods, it occurred more mildly in the PFA-AMeX samples. In conclusion, the PFA-AMeX method is good with respect to morphology and RNA quality, which makes it a promising tool for DNA microarrays combined with laser-capture microdissection, and if the appropriate RNA quality criteria are used, the capture of credible GeneChip data is well over 80% efficient, at least in human prostate specimens. 相似文献
394.
Kusakawa S Tohei A Jaroenporn S Watanabe G Taya K 《The Journal of reproduction and development》2007,53(3):545-554
Glutamate is the dominant excitatory neurotransmitter in a large number of physiological processes including neuroendocrine regulation. Some pharmacological studies have shown that different subtypes of glutamate receptor, such as the N-methyl-D-aspartic acid (NMDA) and alpha-amino-3-hydroxy-5-methy-4-isoxazolepropionic acid (AMPA) receptors, are involved in stress-induced adrenocorticotropin (ACTH) and prolactin secretion. However, the roles of the respective glutamate receptors and the mechanism of ACTH and prolactin secretion during stress via these receptors have not been investigated in detail. In the present study, we evaluated the role of AMPA-type glutamate receptor in ACTH and prolactin regulation under restraint stress in adult male rats. Male rats pretreated with a selective AMPA receptor antagonist, 2, 3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX; 50 microg), through a lateral ventricle cannula were stressed by immobilization. Administration of NBQX inhibited ACTH and prolactin secretion in response to restraint stress. However, NBQX had no significant effects on the activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine biosynthesis, as measured by the accumulation of 3, 4-dihydroxyphenylalanine (DOPA). In addition, administration of NBQX suppressed stress-induced prolactin secretion in the male rats pretreated with alpha-MT, an inhibitor of dopamine synthesis, and infused with dopamine solution (2.5 microg/200 microl/10 min). These results indicated that the effects of NBQX on prolactin secretion might be mediated by non-dopamine mechanisms. The contents of corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) in the median eminence (ME) of the male rats decreased during restraint stress; however, the fluctuations in CRH and AVP were eliminated by NBQX administration. These results suggest that stress-induced ACTH and prolactin release mediated by neurotransmission via AMPA receptors might be partly attributable to hypophysiotropic regulatory factors in the hypothalamus. 相似文献
395.
Simple analytical method for organophosphorus pesticide determination in unpolished rice, using removal of fats by zinc acetate 总被引:1,自引:0,他引:1
K Adachi N Ohokuni T Mitsuhashi 《Journal of the Association of Official Analytical Chemists》1984,67(4):798-800
A rapid and simple method is developed for the determination of organophosphorus pesticides in unpolished rice. The new method incorporated acetonitrile-water (1 + 1) extraction, removal of fats by zinc acetate, and further cleanup on an activated charcoal chromatographic column. The higher fatty acids in the extract react rapidly with zinc acetate to form insoluble zinc carboxylates, which precipitate. Additional interferences were cleaned up on an activated charcoal chromatographic column, and organophosphorus pesticides adsorbed on the activated charcoal were eluted with acetone-hexane. Dimethoate is not retained on the activated charcoal and must be extracted with dichloromethane from the first acetonitrile-water eluate. Pesticides are measured by flame photometric gas chromatography. Recoveries from 50 g unpolished rice samples fortified with 5-50 micrograms diazinon, 6-30 micrograms parathion, 8-40 micrograms fenitrothion and IBP, 10-50 micrograms dimethoate and fenthoate, 20-100 micrograms malathion, or 40-200 micrograms EPN ranged from 75.7 to 95.8%. 相似文献
396.
Ohara Toshimasa Uno Itsushi Wakamatsu Shinji Murano Kentaro 《Water, air, and soil pollution》2001,130(1-4):295-300
Water, Air, &; Soil Pollution - During the spring, the high concentrations of ozone (O3) and aerosols are frequently observed aloft in Japan. Such episodes supposedly are caused by the... 相似文献
397.
Yukiko ITO Shinji TOKI Takashi OMORI Hiroshi IDE Ryuichi TATSUMI Jun-ichi WAKAMATSU Takanori NISHIMURA Akihito HATTORI 《Animal Science Journal》2004,75(1):59-65
The solubility of skeletal muscle myofibrillar proteins in water was examined. The solubility of the proteins was found to be sensitive to ionic strength and pH of the solution. At the ionic strength of less than 12 mM and neutral pH, more than 80% of myofibrillar proteins were solubilized. Heating at a temperature of more than 70°C was required for the proteins to retain their solubility. The solubility of freeze‐dried protein powder prepared from water‐soluble myofibrillar proteins was also examined, and it was found that addition of trehalose and heating were essential for re‐solubilization in water. Amino acid composition of water‐soluble myofibrillar proteins was found to be almost the same as that of myofibrillar proteins. 相似文献
398.
Makrai L Kobayashi A Matsuoka M Sasaki Y Kakuda T Dénes B Hajtós I Révész I Jánosi K Fodor L Varga J Takai S 《Veterinary microbiology》2008,131(3-4):318-323
Rhodococcus equi has been isolated from the submaxillary lymph nodes of domesticated pigs, but little is known about the presence of R. equi in wild boars. The aim of the study was the evaluation of the incidence of R. equi in wild boars and the characterisation of them. Of 482 submaxillary lymph nodes of wild boars shot in 39 settlements throughout Hungary, R. equi was isolated from 60 specimens, and plasmid types of 82 isolates were examined. The isolates were tested for the presence of 15-17-kDa (VapA) and 20-kDa virulence-associated protein antigen (VapB) genes by polymerase chain reaction (PCR). Plasmid DNAs were isolated and analysed by digestion with restriction endonucleases to estimate size and compare their polymorphisms. None of the 82 isolates contained vapA gene but 21 isolates (25.6%) were positive for vapB gene showing 827bp product of the expected size in the PCR amplification. Sixty-one strains (74.4%) did not contain plasmid. The 21 isolates of intermediate virulence contained virulence plasmids that were identified as types 1 (1 isolate), 5 (16 isolates), 21 (1 isolate), and three new distinct plasmid variants (1-1-1 isolate), respectively. On the basis of restriction digestion patterns of plasmid DNAs, we tentatively designated the new variants as types 25-27, respectively. The prevalence of R. equi strains of intermediate virulence among the isolates originated from the submaxillary lymph nodes of wild boars (25.6%) is very similar to those of domestic pigs (26.8%) in Hungary, and plasmid type 5 is the predominating one in both groups. This is the first report of isolation of VapB-positive R. equi from wild boars in the world. 相似文献
399.
Harakawa S Nedachi T Hori T Takahashi K Tochio K Inoue N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):611-613
The aim of the present study was to estimate whether rat sense exogenous electric field (EF) including one used in our previous studies. Employing a conditioned place aversion response paradigm based on an aversive behavior against light environment, alteration in both voluntary behavior of Wistar rat to a 50 Hz sinusoidal EF was examined. Following conditioning without EF, the times spent in white place in rats was significantly shortened (P<0.05). While, such changes were not shown in rats conditioned with EF. Thus, it was considered that the aversion response to light environment was interfered by exposure to EF. An interference in recognition of brightness via EF induced effect to visual system or in learning system via direct effect to central nerve system was considerable as a factor for EF-induced effect. In addition, it was remained that rat possibly sense exposure to EF as preferable. In order to confirm which factor functioned, further studies are needed. 相似文献
400.
Takai H Miyoshi A Yamazaki M Adachi K Katagiri K Arakawa H Katsuyama K Ito T Fujii E Hayashi S Kato A Suzuki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):943-950
Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells, and expected to restore cardiac function for patients with coronary artery diseases as a consequence of progression of atherosclerosis. Safety issues related to the administration of G-CSF to these patients, however, are still under study. The animal model for atherosclerosis was produced by feeding miniature swine a high-cholesterol diet for 3 months. G-CSF (5 or 10 microg/kg/day) was given to the animal model by daily subcutaneous injections for 10 days and 20 main arteries were evaluated pathologically. In addition, the general toxicological effects were studied on clinical signs, body weight, hematology, blood chemistry and pathology. In the G-CSF-treated groups, a variety of changes related to the major pharmacological activity of G-CSF including an increase in white blood cell (WBC) counts were observed. In many arteries, atherosclerotic lesions similar to Type I-V of the proposed classification by the American Heart Association were observed. No effects of the G-CSF treatment were seen on the histopathological findings, incidence, severity or distribution of atherosclerotic lesions. In addition, no infiltration of neutrophils to the lesions was observed. These findings suggest that the administration of G-CSF causes neither exacerbation or modification of atherosclerotic lesions nor adverse changes despite that a sufficient increase in WBC counts could be achieved in the peripheral blood. 相似文献