Deoxynivalenol in hard red winter wheat: relationship between toxin levels and factors that could be used in grading

A study was made of deoxynivalenol (DON) incidences and levels in 1982 hard red winter (HRW) wheat grown in areas of Nebraska and Kansas known to have scabby wheat. Samples of wheat harvested in the areas were collected from elevators and analyzed for DON by gas chromatography with an electron capture detector. Of the 161 samples analyzed, 42% contained less than or equal to 1 ppm; 68% contained less than or equal to 2 ppm; 90% contained less than or equal to 4 ppm. There were differences in the occurrence of DON in the 5 areas identified in eastern Nebraska and Kansas. The mean level of DON decreased from north to south in these areas in the following order: 2.81, 2.73, 2.05, 1.52, and 0.83 ppm. An area in north central Kansas had a mean level of DON of 0.50 ppm. Correlations were made between DON incidences and levels in HRW wheat and factors used in grading wheat. The occurrence of DON was highly correlated with percent total kernels damaged by mold, percent total defects, and percent total scab damage.     

Copper Solubility and Speciation of In Situ Contaminated Soils: Effects of Copper Level,pH and Organic Matter

This study attempts to identify the soil properties controlling the fractionation of copper into various soil pools and determine the influence of pH and metal loading on soil-solution free copper activity (pCu²⁺). The pCu²⁺ was determined in 0.01 M CaCl₂ soil extracts using a copper ion selective electrode. We analyzed a wide variety of soils: urban, agricultural and forest soils from the Province of Québec, New York State and Denmark. The pCu²⁺ ranged from 12.21 to 6.18. The relationships among pCu²⁺, total soil copper, total dissolved copper and soil pH are studied for their variability within and between sites as well as for the whole data set. Regression equations are presented for predicting soluble copper as a function of total soil copper and also for predicting pCu²⁺ as a function of total soil copper and soil pH.     

Estimation of density—Moisture—Stress functions from uniaxial compression of unsaturated, structured soils

A quasi-theoretical soil compaction model is presented which gives good estimates (r²=0.980−0.995, P<0.001) of the complete density-stress compression line for soils of variable initial moisture content under static loads from 0 to 1.0 MPa. The 3 unknown parameter coefficients of the generalized model equation are shown to be highly correlated to several soil properties such as moisture content, pre-compression (initial) void ratio, texture, organic matter content ans the Atterberg consistenct limits. A 3-tier classification for partition consisteny limits. A-tier claasificaton for pationing soils into groupings of response to compressive stress is proposed for soil compaction modeling. This categorization is based on soil plasticity and the existence of a “compaction threshold“ sensitivity threshold” in most soils.     

Effect of Fusarium roseum corn culture containing zearalenone on early pregnancy in swine

A corn culture of Fusarium roseum was added to a standard corn-soybean swine gestation ration. Low, middle, and high dosage mixed feeds contained 7, 38, and 64 mg of zearalenone/kg of feed (7, 38, and 64 ppm) and 0.5, 2.5, and 4.5 mg of deoxynivalenol/kg, respectively. Control feed was the standard ration without added F roseum corn culture. Mature gilts were bred by natural service and fed control or F roseum molded feed from 3 to 34 days after breeding. The main effect of the molded feed was an inhibition of fetal development, with decreased numbers of fetuses present in treated animals at slaughter (38 to 43 days after breeding). Normal litters were present in 7 of 8 control animals, in 2 of 4 gilts given the low-dosage feed, in 1 of 4 gilts given the medium dosage, and in 0 of 4 given the high-dosage feed. Corpora lutea were maintained in all treated animals, as evidenced by serum progesterone concentrations. Serum estradiol concentrations were decreased in gilts in the middle- and high-dosage groups. The genital system of the gilts fed low- and middle-dosage feeds had a gross and microscopic appearance similar to that of the pregnant controls and reflected prolonged progesterone stimulation. Morphologic changes in the genital system of the high-dosage group were intermediate between changes induced by progesterone and those induced by estrogen. Clinical signs of hyperestrogenism and partial feed refusal were noticed in only some of the high-dosage group animals.     

Hematological profiles in dairy cows treated with recombinant bovine somatotropin.

Recombinant bovine somatotropin (bST) was administered at 0, 10.3, or 20.6 mg per cow per day to 32 Holsteins for 38 wk. Fifteen currently treated cows had been treated in the previous lactation. Eighteen hematological variables and blood concentration of insulin-like growth factor I (IGF-I) were measured at five sample periods. The objectives of the study were to test the effects of bST treatments on hematological profiles and to relate blood IGF-I concentration to these variables. Results indicated little influence (P greater than .10) of previous bST treatments on hematological profiles measured in the current lactation. Current bST treatments, however, altered hematocrits (P = .10), mean corpuscular volume (P = .03), mean corpuscular hemoglobin (P = .009), and fraction of blood lymphocytes (P = .09). A week x bST treatment interaction also contributed to variation in hematocrit (P = .09), mean corpuscular hemoglobin (P = .05), fraction of neutrophils (P = .02), and fraction of lymphocytes (P = .04). Blood IGF-I concentration influenced fractions and counts of neutrophils (P = .06, .09), lymphocytes (P = .04, .02), and monocytes (P = .08, .05). Further in vivo and in vitro studies are warranted because this preliminary evidence suggests that bST and(or) IGF-I contribute to regulation of hematopoiesis in mature dairy cows.     

Comparison of three serologic techniques for detection of antibody to Mycoplasma pulmonis

A comparison of three micro-assay detection systems for naturally occurring and experimentally induced rat antibody to Mycoplasma pulmonis was performed. The micro-enzyme linked immunoabsorbent assay was found superior to counter immunoelectrophoresis or complement fixation techniques when comparing sensitivity, duration of antibody response, or technical requirements. The primary immune response was routinely detected at 3 weeks after antigen exposure when using the enzyme linked or complement fixation assays.     

Effect of Fusarium roseum (Gibberella zea) on pregnancy and the estrous cycle in gilts fed molded corn on days 7-17 post-estrus

A laboratory produced corn culture of Fusarium roseum (Gibberella zea) was fed as 10% of the total diet to bred and non-bred gilts on days 7-17 postestrus. The corn culture contained both zearalenone and deoxynivalenol by analysis, and caused considerable feed refusal. Although 6 of 10 bred gilts fed the corn culture appeared to be pregnant by ultrasound testing between days 40-50 post-breeding, none of them farrowed. Progesterone analyses indicated that none of the 10 gilts cycled normally during the 20 week observation period. Three maintained high serum progesterone (greater than 10 ng/ml) during the entire period. Progesterone analyses were similar in 5 non-bred gilts fed the corn culture in that none of them cycled normally in 20 weeks. The retention or loss of corpora lutea could not be attributed to levels of luteinizing hormone, as no relationship was found between concentrations of progesterone and luteinizing hormone. This experiment indicates that limited dietary exposure of female swine to Fusarium roseum infected corn can result in embryonic loss and disruption of normal reproductive cycling for an extended length of time.     

Quantified evaluation of agricultural soil capability at the local scale: a GIS-assisted case study from Ontario, Canada

Abstract. Rural agricultural areas in southern Ontario, Canada, with potential for aggregate extraction have become a focus of conflict over proposed land use change. Geophysical and soil physical field measurements were used to map soil variation for quantitative land evaluation at the farm level. Apparent electrical conductivity of terrain was shown to be strongly correlated with depth to the groundwater table on two separate test sites. A digital terrain model was used to create thematic maps of the predicted pre-growing season soil water regime by contouring irregularly spaced electromagnetic survey and soil inspection points. Overlay analysis with a geographical information system (GIS) was used to produce an agricultural soil capability map for crop production. Adoption of a larger map scale provided significant refinement in detail over the published Canada Land Inventory soil capability ratings for agriculture, but both showed that Class 2 soils are dominant. The approach can improve the reproducibility of land capability assessments.