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71.
Michael Elliott Richard L. Elliott Norman F. Janes Bhupinder P. S. Khambay David A. Pulman 《Pest management science》1986,17(6):691-700
Over 100 benzyl esters of pyrethroidal acids were synthesised and tested for insecticidal activity to establish detailed structure–activity relationships in compounds with side-chains similar to those in the natural pyrethrins. Alkenyl, and corresponding alkynyl, side-chains were effective, both at the 3- and 4-positions, as were side-chains with extended substitution in either E or Z forms. A cyano group at the α-position increases activity if the side-chain is at C-3, but lowers it drastically if the substituent is at C-4. Similarly, methyl groups at C-2 and/or C-6 may increase activity whether the unsaturated side-chain is at C-3 or C-4, but only in the absence of an α-cyano group. 相似文献
72.
Richard L. Bruggers 《Phytoparasitica》1994,22(3):183-187
Crop protection research in the Tropics is always interesting and challenging, and usually professionally rewarding. Once involved, researchers often find it difficult to leave international research settings. However, the wise field scientist, when choosing such a career, will make Barley ’s (3) “The Innocent Anthropologist ” required reading and purchase a very good pair of binoculars and a local bird guide. Even the most resourceful individual is going to have a considerable amount of time to add birds to one ’s life list, improve species distribution maps, or possibly even identify a new species while waiting for the key to be found, the vehicle to show up, or the mud to dry out. 相似文献
73.
Dmitri Andreev Thomas Rocheleau Thomas W. Phillips Richard W. Beeman Richard H. ûrench-Constant 《Pest management science》1994,41(4):345-349
A molecular diagnostic was used to examine the conservation of cyclodiene resistance associated mutations between different strains of Tribolium castaneum (Herbst.). An improved insecticide bioassay for discrimination between resistant genotypes was developed and seven resistant strains were established from five different continents. In order to develop a molecular diagnostic a partial cDNA of the cyclodiene insecticide resistance gene Rdl, a γ-aminobutyric-acid-gated chloride-ion channel, was cloned and sequenced. This cDNA spans exon 7, the region containing the resistance-associated mutation, and part of exon 8. An ‘allele-specific’ oligonucleotide primer, carrying the resistance-associated mutation at its 3′ end, was used in combination with a flanking ‘allele-independent’ primer in the polymerase chain reaction to selectively amplify a single resistance-associated mutation from all seven strains collected worldwide. The implications of these findings for the population genetics of insecticide resistance and its management in pest insects via quarantine are discussed. 相似文献
74.
75.
Qu L Chen Y Wang X Scalzo R Davis JM 《HortScience : a publication of the American Society for Horticultural Science》2005,40(5):1239-1242
We investigated patterns of variation in alkamides and cichoric acid accumulation in the roots and aboveground parts of Echinacea purpurea (L.) Moench. These phytochemicals were extracted from fresh plant parts with 60% ethanol and quantified by high performance liquid chromatography (HPLC) analysis. Concentrations of alkamides and cichoric acid were measured on a dry-weight basis (mg·g(-1)). For total alkamides, concentrations among individual plants varied from 5.02 to 27.67 (mean = 14.4%) in roots, from 0.62 to 3.42 (mean = 1.54) in nearly matured seed heads (NMSH), and 0.22 to 5.25 (mean = 0.77) in young tops (about ? flower heads, ? leaves, and ? stems). For cichoric acid, concentrations among individual plants varied from 2.65 to 37.52 (mean = 8.95), from 2.03 to 31.58 (mean = 10.9), and from 4.79 to 38.55 (mean = 18.88) in the roots, the NMSH, and the tops, respectively. Dodeca-2E, 4E, 8Z, 10E-tetraenoic acid isobutylamide and dodeca-2E, 4E, 8Z, 10Z-tetraenoic acid isobutylamide (alkamides 8/9) accounted for only 9.4% of the total alkamides in roots, but comprised 87.9% in the NMSH, and 76.6% in the young tops. Correlations of concentrations of alkamides or cichoric acid between those of roots and those of the NMSH were not statistically significant, and either within the roots, the NMSH, and the young tops. However, a significant negative correlation was observed between the concentration of cichoric acid in the roots and in young tops, and a significant positive correlation was observed between total alkamide concentration in the roots and cichoric acid concentration in the young tops. These results may be useful in the genetic improvement of E. purpurea for medicinal use. 相似文献
76.
Qu L Wang X Chen Y Scalzo R Widrlechner MP Davis JM Hancock JF 《HortScience : a publication of the American Society for Horticultural Science》2005,40(6):1843-1845
Seed germination patterns were studied in E. purpurea (L.) Moench grouped by seed source, one group of seven lots from commercially cultivated populations and a second group of nine lots regenerated from ex situ conserved wild populations. Germination tests were conducted in a growth chamber in light (40 μmol·m(-2)·s(-1)) or darkness at 25 °C for 20 days after soaking the seeds in water for 10 minutes. Except for two seed lots from wild populations, better germination was observed for commercially cultivated populations in light (90% mean among seed lots, ranging from 82% to 95%) and in darkness (88% mean among seed lots, ranging from 82% to 97%) than for wild populations in light (56% mean among seed lots, ranging from 9% to 92%) or in darkness (37% mean among seed lots, ranging from 4% to 78%). No germination difference was measured between treatments in light and darkness in the commercially cultivated populations, but significant differences were noted for treatments among wild populations. These results suggest that repeated cycles of sowing seeds during cultivation without treatments for dormancy release resulted in reduced seed dormancy in E. purpurea. 相似文献
77.
Peter J. Holloway Wilson W.-C. Wong Hazel J. Partridge David Seaman Richard B. Perry 《Pest management science》1992,34(2):109-118
The influence of a number of commercial nonionic polyoxyethylene surfactants on the foliar penetration and movement of two systemic fungicides, ethirimol and diclobutrazol, was studied in outdoor-grown wheat plants at different growth stages and post-treatment temperatures in two consecutive growing seasons. Both fungicides were applied as ca 0·2 μl droplets of aqueous suspension formulations containing 0·5 g litre?1 of 14C-labelled active ingredient; surfactants were added to these suspensions at concentrations ranging from 0·2-10 g litre?1. To achieve optimum uptake of each fungicide the use of surfactants with different physicochemical properties was required. For diclobutrazol, a lipophilic compound, uptake of radiolabel was best with surfactants of low mean molar ethylene oxide (E) content (5-6) but it was necessary to use concentrations of ca 5 g litre?1 to attain this. The surfactant threshold concentration for uptake enhancement of radiolabel from ethirimol formulations (< 2 g litre?1) was much lower than that for diclobutrazol but surfactants with E contents > 10 induced the greatest amount of uptake. For both fungicides, surfactants with an aliphatic alcohol hydrophobe were generally more efficient in promoting their uptake than those with a nonylphenol moiety. The sorbitan-based surfactant ‘Tween 20’ proved to be an effective adjuvant only for the ethirimol formulation; the uptake enhancing properties of the block copolymer ‘Synperonic PE/F68’ were weak. Uptake performance could not be related to the spreading properties of the respective formulations on the wheat leaf surface or to differences in solubilisation of the two fungicides by the surfactants. Although surfactants could substantially increase the amount of acropetal transport of radiolabel from both fungicides, none of those tested specifically promoted it; a constant proportion of the radioactive dose absorbed by a treated leaf was usually exported away from the site of application. The results are discussed in the light of current theories about the mode of action of surfactants as spray adjuvants. 相似文献
78.
Alan R. McCaffery Jonathan W. Holloway Richard T. Gladwell 《Pest management science》1995,44(3):237-247
Spontaneous neuronal activity was recorded from the peripheral nerves of third-instar larvae of strains of Heliothis virescens (F.) obtained directly from cotton fields in the USA. Following a control period the preparations were exposed to increasing concentrations of cis-cypermethrin in a cumulative dose-response assay. A positive response was defined as an increase of at least five-fold in the rate of neuronal activity over that seen during the control period. Up to 35 individuals of each strain were assayed and the responses used to construct a phenotypic profile categorising the individuals from nerve-susceptible to highly nerve-insensitive. An EC50 for the action of cis-cypermethrin was also obtained. There was a positive, significant correlation between non-synergisable resistance to cypermethrin and nerve insensitivity as defined in the neurophysiological assay. It was shown that nerve insensitivity to cypermethrin increased throughout the cotton-growing season. 相似文献
79.
The dose-response curve was quantified for the avian repellency of cinnamamide, a derivative of the plant secondary compound cinnamic acid, using a three-day, ‘short-term no-choice’ test. This test was designed to resemble more closely the availability of alternative food in the birds' natural environment than in previous no-choice studies. Cinnamamide reduced food consumption by feral pigeons (Columba livia Gmelin) at concentrations as low as 6.17 μmole g?1 food (0.09% w/w) and this reduction increased with concentration. An R50 index, the concentration at which food consumption was reduced by 50% was interpolated from the dose-response curve as 18.4 (±0.38) μmole g?1 food 10.26% w/w). This index can now be matched with those of related compounds in future structure-activity studies. The birds' response to cinnamamide changed over the three-day trial with respect to concentration. Repellency declined at concentrations < R50 and increased at concentrations > R50. This suggests that pigeons become habituated to cinnamamide at low concentrations but at high concentrations they may develop a conditioned aversion. This study provides quantitative confirmation of previous laboratory and field demonstrations of cinnamamide's effectiveness as a non-lethal chemical repellent suitable for reducing avian pest damage. 相似文献
80.
The effect of pyrimethanil on the levels of cell wall degrading enzymes secreted by Botrytis cinerea Pers. was investigated in diseased plant tissues and in liquid B. cinerea cultures. Total proteinase activity isolated from infected carrot slices which were treated with 5.0 μM pyrimethanil was decreased by 76%, 3 d after inoculation. Polygalacturonase, cellulase, proteinase and laccase activities were all decreased in the medium of three day-old cultures grown in the presence of pyrimethanil. The pyrimethanil concentrations resulting in 50% reduction in total enzyme activities (IC50) were approximately 0.25 μM for polygalacturonase, cellulase and proteinase, and approximately 1.0 μM for laccase. No significant growth inhibition was observed at these pyrimethanil concentrations. Pyrimethanil did not inhibit the enzymes directly, nor did it inhibit the synthesis of cytosolic proteins. Therefore, it was proposed that the fungicide inhibits protein secretion at a post-translational stage in the secretory pathway. Large differences were found in the effects of pyrimethanil on the growth of B. cinerea in liquid cultures and on agar plates, depending on the composition of the medium. In liquid media containing cellulose and protein as carbon and nitrogen sources, growth inhibition occurred at 5.0 μM pyrimethanil, whilst no growth inhibition was observed with 50 μM pyrimethanil in malt extract. Similarly, growth occurred on potato/dextrose agar (PDA) at 0.5 μM pyrimethanil, but no growth was seen at this concentration on agars containing cellulose and protein. Thus it appears that pyrimethanil is most active in media where the fungus has to utilise extracellular enzymes to mobilise the nutrients it requires for growth. 相似文献