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71.
进一步探讨了制定科研评价体系的原则、方法等,并结合目前我国研究型大学的实际情况初步提出了一套科研评价指标体系。 相似文献
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本文对会计电算化发展中存在的一些问题提出了对策与建议,并针对财会工作的自身特点,从程序控制和制度控制两个方面阐述了电算化会计信息下的内部控制方法。 相似文献
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The effect of concurrent cecal coccidiosis infections on severity of Histomonas meleagridis (blackhead disease) in chickens was investigated in a series of experiments. Cecal lesions from H. meleagridis were severe in all inoculated control groups and did not appear to be affected by the introduction of Eimeria tenella infection. However, the severity of liver lesions and number of birds positive for liver lesions of H. meleagridis increased significantly with the presence of E. tenella. The increase was similar when 10(3) or 10(4) oocysts of E. tenella were given and was the same when oocysts were given at the same time as H. meleagridis or 4 days prior. The liver lesions increased directly as doses of H. meleagridis increased from 7.5 x 10(3) cells to 30, 100, or 300 x 10(3) when E. tenella was given along with H. melelagridis but not when H. meleagridis was given alone. Administration of a live coccidiosis vaccine containing very low levels of E. tenella also gave a significant boost to liver lesions but at a much lower level than that observed with larger doses of E. tenella. The positive relationship between infections of cecal coccidiosis and H. meleagridis in chickens suggests that such dual exposure may contribute to increased clinical outbreaks of blackhead disease in chickens under field conditions. 相似文献
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A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
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两种光周期下矮牵牛‘幻想粉红’生长发育特性的研究 总被引:3,自引:2,他引:3
将矮牵牛‘幻想粉红’分别培养在长日照和短日照条件下, 观测其始花期及株高、株幅、分枝数和开花数的变化; 将短日照下培养的‘幻想粉红’分别于2、4、6、8、10、12、14、16片真叶时移入长日照下进行培养, 观测从长日照处理至第1朵花开放的时间。结果分析表明: 长日照下, ‘幻想粉红’株型松散, 短日照下生长紧凑; ‘幻想粉红’为量性长日照植物, 短日照下要达到较大的营养生长量才能开花; ‘幻想粉红’的幼龄期约在8片真叶期结束。初步建议在8叶期之前采用短日照处理‘幻想粉红’,以得到紧凑的株型, 而在8叶期以后给予长日照, 以促进开花。 相似文献
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杨桃果实生长发育过程中营养品质的变化 总被引:8,自引:0,他引:8
杨桃(Averrhoa arambola L.)为华南地区重要的热带水果。本试材为华南农业大学园艺分场种植的3年生盆栽马来西亚甜杨桃,砧木为酸杨桃。在杨桃的开花期,选择生长势、开花数量和时间较一致45株为试材,再选择受精时间一致的果实做标记。采样时问从开花后(2003年7月15日)至杨桃充分成熟(2003年10月25)。测定杨桃果实中的糖(蒽酮比色法)、有机酸(HPLC法)、维生素C(2,6-二氯靛酚钠滴定法)和单宁(高锰酸钾滴定法)的含量。 相似文献