Determination of growth-stage-specific crop coefficients (KC) of maize and sorghum

A ratio of crop evapotranspiration (ET_C) to reference evapotranspiration (ET_O) determines a crop coefficient (K_C) value, which is related to specific crop phenological development to improve transferability of the K_C values. Development of K_C can assist in predicting crop irrigation needs using meteorological data from weather stations. The objective of the research was conducted to determine growth-stage-specific K_C and crop water use for maize (Zea Mays) and sorghum (Sorghum bicolor) at Texas AgriLife Research field in Uvalde, TX, USA from 2002 to 2008. Seven lysimeters, weighing about 14 Mg, consisted of undisturbed 1.5 m × 2.0 m × 2.2 m deep soil monoliths. Six lysimeters were located in the center of a 1-ha field beneath a linear-move sprinkler system equipped with low energy precision application (LEPA). A seventh lysimeter was established to measure reference grass ET_O. Crop water requirements, K_C determination, and comparison to existing FAO K_C values were determined over a 3-year period for both maize and sorghum. Accumulated seasonal crop water use ranged between 441 and 641 mm for maize and between 491 and 533 mm for sorghum. The K_C values determined during the growing seasons varied from 0.2 to 1.2 for maize and 0.2 to 1.0 for sorghum. Some of the values corresponded and some did not correspond to those from FAO-56 and from the Texas High Plains and elsewhere in other states. We assume that the development of regionally based and growth-stage-specific K_C helps in irrigation management and provides precise water applications for this region.     

An assessment of the concentrations of PCDDs/Fs in contaminated bottom sediments and their sources and ecological risk

Purpose

The purpose of the study was to determine the levels of polychlorinated dibenzo-p-dioxin and polychlorinated dibenzofuran (PCDD/F), two types of persistent organic pollutant (POP), in an urban retention reservoir located in an industrial zone within a coal-mining region. It also assesses the potential ecological risk of the PCDDs/Fs present in bottom sediments and the relationship between their content and the fraction of organic matter.

Materials and methods

The sediment samples were collected from Rybnik Reservoir, located in the centre of the Rybnik Coal Region, Silesia, one of Poland’s major industrial centres. Seventeen PCDD/F congeners in the surface of the sediments were analysed using high-resolution gas chromatography and high-resolution mass spectrometry (HRGC/HRMS).

Results and discussion

The toxic equivalency (TEQ) of the PCDDs/Fs in the sediments ranged from 1.65 to 32.68 pg TEQ g^?1. PCDDs constituted 59–78% of the total PCDDs/Fs, while the PCDFs accounted for 22–41%. The pattern of PCDD/F congeners in the sediments was dominated by OCDD. However, the second-most prevalent constituents were OCDF and ∑HpCDFs in the low TOC sediment (< 10 g TOC kg^?1), but HpCDD in the rich TOC samples (> 10 g TOC kg^?1). PCDD/F concentrations in the sediment samples were 2- to 38-fold higher than the sediment quality guidelines limit, indicating high ecological risk potential. Although a considerable proportion of PCDDs/Fs in the bottom sediments from the Rybnik Reservoir were derived from combustion processes, they were also obtained via transport, wastewater discharge, high-temperature processes and thermal electricity generation. The PCDD/F concentrations were significantly correlated with all fractions of organic matter; however, the strongest correlation coefficients were found between PCDDs/Fs and humic substances. Besides organic matter, the proportions of silt/clay fractions within sediments played an important role in the transport of PCDDs/Fs in bottom sediments.

Conclusions

The silt/clay fraction of the bottom sediments plays a dominant role in the movement of PCDDs/Fs, while the organic matter fraction affects their sorption. The results indicate that the environmental behaviour of PCDDs/Fs is affected by the quantity and quality of organic matter and the texture of sediments.

     

Root morphological traits of winter wheat under contrasting environments

Roots strongly influence the growth and yield of field crops. We characterized root morphological traits of 10 winter wheat varieties in order to determine the extent they were influenced by the environments and impacted grain yield under two irrigation regimes at Bushland (a cooler, drier site with clay loam soil) and Uvalde (a warmer, wetter site with clay soil) in Texas, USA, from 2015 to 2017. Major root traits, including root diameter, specific root length (SRL), root surface area (SSA), tissue mass density (TMD), root length density (RLD), and root weight density, were measured and related to one another and to grain yield. RLD of wheat decreased but SRL and SSA increased with soil depth. Irrigation was second to environment in affecting root traits. Compared with Uvalde, the environment of Bushland promoted deeper root growth, higher TMD, but reduced SRL and SSA. Water deficit inhibited RLD and root: shoot ratio at Bushland, but moderately promoted them at Uvalde. Both SRL and RLD were positively associated with grain yield, with the former relation stronger under drought. The dichotomy of “conservative” versus “acquisitive” root strategy partially explained the variations of root traits of winter wheat in contrasting environments.     

CORRELATION BETWEEN TISSUE AND SUBSTRATE SILICON CONCENTRATION OF GREENHOUSE PRODUCED ORNAMENTAL SUNFLOWERS

The beneficial effects of the “nonessential” plant nutrient, silicon (Si), are well documented for several agricultural crops. Soilless growth media used in greenhouse production provides only limited amounts of available Si to container grown plants compared to plants grown in mineral-derived soils. Si supplementation is documented to increase resistance to biotic and abiotic stresses in greenhouse crops, which accumulate Si in their tissues. However, optimum Si fertilization rates and acceptable Si levels in tissues and substrate have not been established for floriculture greenhouse production. For this study ornamental sunflower (Helianthus annuus L. ‘Ring of Fire’) was used to investigate the relationship between substrate Si and accumulation of Si in the tissues of plants grown in a peat-based media. Weekly substrate drenches of potassium silicate (KSiO₃), substrate incorporation of KSiO₃ hydrous powder, or rice husk ash were used as Si supplements. Overall, leaf, stem, and flower Si concentrations of Si-supplemented plants increased compared to nonsupplemented controls. A positive correlation was observed between substrate Si concentration and leaf Si concentration for all three Si sources used in this study. Therefore, leaf tissue is the most appropriate tissue to sample in order to determine the availability of Si in a substrate and could be used to establish acceptable Si levels for soilless greenhouse floriculture.     

Perna canaliculus Lipid Complex PCSO-524? Demonstrated Pain Relief for Osteoarthritis Patients Benchmarked against Fish Oil,a Randomized Trial,without Placebo Control

Osteoarthritis (OA) typically generates pain, reduced mobility and reduced quality of life. Most conventional treatments for osteoarthritis, such as non-steroidal anti-inflammatory drugs (NSAIDs) and simple analgesics, have side effects. PCSO-524™, a non polar lipid extract from the New Zealand Green Lipped Mussel, is rich in omega-3 fatty acids and has been shown to reduce inflammation in both animal studies and patient trials. This OA trial examined pain relief changes in relation to quality of life and safety of use for OA patients who took PCSO-524™ compared with patients who took fish oil (containing an industry standard EPA-18% and DHA-12% blend). PCSO-524™ patients showed a statistically significant improvement compared with patients who took fish oil. There was an 89% decrease in their pain symptoms and 91% reported an improved quality of life. Patients treated with fish oil showed significantly less improvement and a greater level of physical discomfort during the study. These results suggest that PCSO-524™ might offer a potential alternative complementary therapy with no side effects for OA patients.     

A Neolithic yew bow in the Polish Carpathians: Evidence of the impact of human activity on mountainous palaeoenvironment from the Kamiennik landslide peat bog

A unique artefact — the fragment of a bow made of yew wood, indicating the hunting activity of prehistoric man — was found within the sediments of the landslide peat bog (at the depth of 330 cm) formed on the Mt. Kamiennik (Polish Flysch Carpathians). The datings of this artefact using ¹⁴C method at ca 3790–3380 cal BC indicate its connection with the activity of the Neolithic man, related to the Funnel Beaker Culture. In the sequence of the peat bog deposits formed since the Atlantic Phase, the palaeoclimatic changes of the Middle- and Late Holocene, as well as traces of human impact in these and subsequent periods were recorded and analysed using palynological, macrofossils, lithological methods, and dated with radiocarbon. Palaeoenvironmental changes identified in the peat horizon bearing the artefact were marked by the delivery of minerogenic, “high energy” deposits (with charcoal) to the peat bog, as well as distinct changes of plant communities. These phenomena confirm a significant human impact caused by the Prehistoric man on landslide areas. On the other hand, the study confirms significant importance of climatic condition for human activity and proves that landslide peat bog deposits are very sensitive records of palaeoenvironmental changes. The archaeological artefact essentially supplements the results of our interdisciplinary palaeoenvironmental study. It is one of the rare Neolithic bows found in the area of Europe, till now.     

Influence of nitric oxide and reactive oxygen species on development of lettuce downy mildew in <Emphasis Type="Italic">Lactuca</Emphasis> spp.

The role of nitric oxide and reactive oxygen species, molecules indispensable for plant-pathogen signalling, was studied in the Lactuca spp.-Bremia lactucae pathosystem. Using a leaf disc model the translaminar effect of various compounds affecting their metabolism was studied by light microscopy. Time course studies revealed a slowdown in the development of B. lactucae (race BL16) infection structures by rutin (scavenger of reactive nitrogen and oxygen species) and SNP (NO donor) within 48 h post inoculation, followed by a retardation of sporulation. Application of the specific NO scavenger, PTIO, accelerated penetration of B. lactucae but had no further effects on the plant-pathogen interaction. Inhibitors of NO synthase (L-NAME) and nitrate reductase (sodium tungstate) did not influence pathogen development. Our results suggest that drastic change in the NO: hydrogen peroxide ratio seems to determine the pathogen’s fate. NO synthase-like activity significantly increased early after B. lactucae challenge in resistant L. virosa. Confocal laser scanning microscopy revealed the accumulation of nitric oxide in the penetrated cells, pointing to a role in the initiation of the hypersensitive reaction. The tips of germ tubes and appressoria of B. lactucae also accumulated NO, suggesting an essential role for this molecule in penetration of the biotrophic pathogen. Additionally, temporal changes in endogenous levels of rutin and quercetin in extracts from Lactuca spp. leaves will be discussed in connection to their role as part of the antioxidative machinery that influences the plants’ susceptibility/resistance to lettuce downy mildew.     

High resistance to pyrethroid insecticides in the Polish pollen beetle (<Emphasis Type="Italic">Meligethes aeneus F.</Emphasis>): the role of oxidative metabolism

Pollen beetle (Meligethes aeneus F.) (PB) causes very serious problems in oilseed rape (Brassica napus var. oleifera L.) cultivations in Poland and other European countries. Pyrethroid insecticides are the most frequently used type for controlling PB in Poland. This chemical group has been used in Poland since the beginning of the 1980’s and its strong selection pressure on PB resulted in some level of resistance of this pest to many active substances. The aim of this investigation was to analyze, with the use of synergists, the main mechanisms responsible for resistance of PB to pyrethroids. The results indicate the main resistance factor in Polish PB populations to be monooxygenase enzymes.     

Perceived predation risk reduces the number of offspring songbirds produce per year

Predator effects on prey demography have traditionally been ascribed solely to direct killing in studies of population ecology and wildlife management. Predators also affect the prey's perception of predation risk, but this has not been thought to meaningfully affect prey demography. We isolated the effects of perceived predation risk in a free-living population of song sparrows by actively eliminating direct predation and used playbacks of predator calls and sounds to manipulate perceived risk. We found that the perception of predation risk alone reduced the number of offspring produced per year by 40%. Our results suggest that the perception of predation risk is itself powerful enough to affect wildlife population dynamics, and should thus be given greater consideration in vertebrate conservation and management.     

Hemoglobins S and C interfere with actin remodeling in Plasmodium falciparum-infected erythrocytes

The hemoglobins S and C protect carriers from severe Plasmodium falciparum malaria. Here, we found that these hemoglobinopathies affected the trafficking system that directs parasite-encoded proteins to the surface of infected erythrocytes. Cryoelectron tomography revealed that the parasite generated a host-derived actin cytoskeleton within the cytoplasm of wild-type red blood cells that connected the Maurer's clefts with the host cell membrane and to which transport vesicles were attached. The actin cytoskeleton and the Maurer's clefts were aberrant in erythrocytes containing hemoglobin S or C. Hemoglobin oxidation products, enriched in hemoglobin S and C erythrocytes, inhibited actin polymerization in vitro and may account for the protective role in malaria.