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201.
Samantha L. Eaton Mary-Jo Anderson Scott Hamilton Lorenzo Gonzlez Jill Sales Martin Jeffrey Hugh W. Reid Mara S. Rocchi Francesca Chianini 《Veterinary immunology and immunopathology》2009,131(1-2):105-109
In order to gain a better understanding of the pathogenesis of scrapie in sheep an experimental model was developed to characterise immune system cells in the minutes following inoculation with scrapie-brain homogenate. Four 1-year-old susceptible (ARQ/ARQ) sheep were inoculated via the subcutaneous route at four different peripheral lymph node (LNs) drainage sites, at specific time points, prior to euthanasia of the sheep. The LNs were removed post-mortem at 30, 90, 180 and 300 min after inoculation. Flow cytometric triple-labelling was carried out on the LN cells and indicated that inoculation of scrapie-brain homogenate adjacent to a lymph node may delay or even inhibit the number of host CD21+ B cells expressed within the first 5 h. Immunohistochemistry was used to attempt detection of the abnormal form of prion protein (PrPsc) in draining LNs adjacent to inoculation sites, with negative results at those time points. 相似文献
202.
Bonacina Cristine Nascimento Andressa Bezerra Barbosa Letícia Neris de Souza Silvia Graciele Hülse Magalhães Hélida Mara 《Journal of Crop Science and Biotechnology》2023,26(2):215-226
Journal of Crop Science and Biotechnology - During cultivation, environmental factors can cause changes in the metabolism of Curcuma longa L. that limit its development. This study evaluated the... 相似文献
203.
Curcuma longa supplementation in the diet of Astyanax aff. bimaculatus in preparation for transport 下载免费PDF全文
Pollyanna de Moraes França Ferreira Juliana Silva Rocha Juliana Rodrigues Gomes Débora Werneck Caldas Maria Tatiana Soares Martins Jerusa Maria de Oliveira Ana Lucia Salaro Jener Alexandre Sampaio Zuanon 《Aquaculture Research》2017,48(8):4524-4532
The physiological state of fish before transportation is an important aspect to be observed when reduction in losses due to mortality is aimed. Various preparative procedures for transport are reported, but little is known about the use of additives in fish diet. In this study, we aimed to evaluate the effect of dietary turmeric (Curcuma longa) supplementation on the tolerance of juvenile Astyanax aff. bimaculatus to transportation stress. The experimental design consisted of six treatments: 0, 20, 40, 60, 80 and 100.0 g kg?1 turmeric. Fish weighing 0.83 ± 0.04 g were distributed in 24 80‐L aquaria at a density of 0.5 fish L?1 and were fed to satiation three times a day for 60 days. After this period, fish were transported for 24 h in plastic bags (15 fish per bag). Using a discontinuous linear response plateau (LRP) model, the lowest estimated concentration of turmeric that maximized the survival rate was 16.67 g kg?1. Quadratic effects of turmeric on blood lactate levels were observed, and the estimated concentration of turmeric required to minimize blood lactate levels was 64.7 g kg?1. We observed quadratic effects of turmeric on superoxide dismutase activity and the concentration of malondialdehyde in fish gills and the turmeric concentrations required to minimize the transport stress effects on these variables were 42.50 and 83.33 g kg?1, respectively. Thus, dietary supplementation with turmeric enhanced the tolerance of juvenile A. aff. bimaculatus to transportation stress. 相似文献
204.
Pollyanna de Moraes França Ferreira Suellen Silva Condessa Juliana Silva Rocha Débora Werneck Caldas Juliana Rodrigues Gomes Maria Tatiana Soares Alex Junio Silva Cardoso Jener Alexandre Sampaio Zuanon 《Aquaculture Research》2017,48(9):4623-4631
With this study, we aimed to assess the safety of using Curcuma longa in fish feed. Juvenile Astyanax aff. bimaculatus (0.83 ± 0.04 g) were kept in 24 80‐L aquaria, at a density of 0.5 fish L?1 for 60 days. Six diets supplemented with 0.0, 20.0, 40.0, 60.0, 80.0 and 100.0 g kg?1 were evaluated. Histopathological analysis of the gills showed that there was an increasing linear effect of turmeric on the frequency of lamellar aneurysm, hypertrophy of mucous cells, oedema and necrosis. There was a quadratic effect of turmeric supplementation on the frequency of fusion and disruption of secondary lamellae, vacuolation and hypertrophy of the epithelial cells, and the estimated values to minimize these variables were 4.63, 25.93 and 24.46 g kg?1 respectively. There was a quadratic effect of turmeric on the gills index (IBRA), and the estimated value to minimize this index was 30.23 g kg?1. No effect of turmeric on the frequency of histopathological changes and organ index in fish liver was observed. Thus, we conclude that turmeric is not hepatotoxic for A. aff. bimaculatus; however, its use is safe at concentrations up to 30.23 g kg?1 diet as a function of its gill toxic effect at higher doses. 相似文献
205.
Pollyanna de Moraes França Ferreira Maria Tatiana Soares Martins Débora Werneck Caldas Juliana Rodrigues Gomes Jerusa Maria de Oliveira Ana Lucia Salaro Juliana Silva Rocha Jener Alexandre Sampaio Zuanon 《Fish physiology and biochemistry》2017,43(3):691-702
The aim of the present study was to evaluate the effect of turmeric (Curcuma longa) as additive in the diet for Astyanax aff. bimaculatus. Fish (0.83 ± 0.04 g) were fed, for 60 days, with six diets containing 0.0, 20.0, 40.0, 60.0, 80.0, and 100.0 g turmeric kg?1 feed. There was an increasing linear effect of turmeric on the thickness of the muscular layer, and height and width of the folds of the intestine. In the liver, a quadratic effect was observed of turmeric on the percentage of hepatocyte cytoplasm and a decreasing linear effect on the percentage of sinusoid capillaries. A quadratic effect was also observed of turmeric on the liver glycogen. There was no effect of turmeric on the antioxidant activity in the liver, carcass composition or productive performance of the fish. Thus, we concluded that Curcuma longa has trophic effects on the epithelium and the muscular layer of the intestine of A. aff. bimaculatus. Additionally, low levels of Curcuma longa cause increased deposition of liver glycogen and high levels cause reduction. 相似文献