山西东部土石山立地区立地类型的划分——山西省立地类型划分与造林模式研究（Ⅺ）

分析了山西东部土石山区的自然地理条件,按亚区的区划原则将该立地区分为太行山北段山地立地亚区、晋东土石山立地亚区和中条山土石山立地亚区3个亚区,并根据立地类型小区、立地类型组和立地类型的划分原则将该立地区划分为9个立地类型小区,51个立地类型组和95个立地类型。     

橡胶树高产抗寒材料云研77-2、云研77-4的选育

利用高产、抗寒力强的亲本进行有性杂交，通过抗寒前哨苗圃抗寒性的选择和初级系比、高级系比，以及生产性试验系统资料的鉴定，云研７７—２和云研７７—４在生长、产量、抗性等方面优于ＧＴ１，特别是云研７７—４抗寒能力强。推荐在云南省西部植胶区的重寒区中规模种植；东部植胶区中寒区试种。     

Comparison of the Distribution of PGP9.5 and NPY in Cryptorchidism and Testicular Tumors in Dogs

The purpose of this study was to analyze the distribution and expression of peptidergic neurotransmitters protein gene product 9.5 (PGP9.5) and neuropeptide Y (NPY) in cryptorchidism and testicular tumors of dogs,compare them with normal testicular tissues of the same age,and provide reference for clinical diagnosis of malignant transformation in testicular tumors of dogs.HE staing,Masson trichrome staining,Gomori silver staining and toluidine blue staining were used to observe the tissue characteristics of reticular fibers,collagen fibers and mast cells.Immunohistochemical SP method and immunofluorescence combined with IPP were used to analyze the expression and localization of PGP9.5 and NPY in tissues.The results showed that the seminiferous epithelium of normal dog testis was composed of 4-7 layers of spermatogenic cells and Sertoli cells,and the distribution of collagen fibers and reticular fibers in interstitial tissue was sparse.The thickness of collagen fibers in the basement membrane of cryptorchidism seminiferous tubules increased,the nucleus of Sertoli concentrated at the base of seminiferous tubules,and the interstitial reticular fibers increased.The tissue structure of testicular tumor was unclear,collagen fibers and reticular fibers were irregularly distributed,and mast cells increased significantly compared with normal and cryptorchid groups.The immunofluorescence results showed that PGP9.5 was moderately positive in Leydig cells of normal testis,no significant expression in spermatogenic cells,strongly positive in Leydig cells and spermatogenic cells of cryptorchidism,and occasional expression in testicular tumors.NPY was occasionally expressed in normal testicular Leydig cells,but not in spermatogenic cells,strong positive expression in Leydig cells and seminiferous epithelium of cryptorchidism,high density and strong positive expression in interstitial vessels,and no obvious expression in testicular tumors.Immunohistochemical statistics showed that the expression of PGP9.5 and NPY in testicular tumor tissue were extremely significantly lower than that in normal group (P<0.01),while the expression of PGP9.5 and NPY in cryptorchidism group were significantly or extremely significantly increased (P<0.05;P<0.01).Therefore,the expression of PGP9.5 and NPY in cryptorchidism of dogs was increased suggesting that the cryptorchidism of dogs had a tendency to develop into a tumor,and was related to the degree of malignant transformation of tumor.     

黑龙江东北虎林园管理策略探讨

2006年，黑龙江东北虎林园的东北虎人工饲养种群超过700只，维持其生存的费用巨大，虎林园的管理策略研究应进一步加强。本文从服务管理、营销管理、人力资源管理、科普教育管理、基建管理五个方面进行了探讨。     

Research Progress of Alternative Splicing and Its Application in Livestock and Poultry Breeding

Alternative splicing(AS) refers to the process of selective splicing across exons or splice junctions during individual development or cell differentiation to produce tissue-or development-stage specific mRNA. Through AS process, organisms can produce many different protein isomers from a single gene and finally increase the proteome diversity. Therefore, AS plays an important role in the regulation of animal growth, development and physiological metabolism. This article not only summarized the types and the detection methods of alternative splicing, but also highlighted its impact on various economic traits of livestock and poultry, and prospected its application prospects in livestock and poultry breeding. This article could provide new ideas for the improvement of livestock and poultry breeding.     

黑土坡耕地横坡垄作对减少径流及土壤有机碳流失的作用

东北黑土坡耕地受土壤侵蚀和习惯顺坡耕作措施的影响,水土流失严重,土壤有机碳含量呈逐年下降趋势.针对东北黑土坡耕地不同垄作措施水土流失及土壤有机碳变化特征不明确的问题,采用田间定位试验的方法,探究了顺坡垄作和横坡垄作对坡耕地水土流失及土壤有机碳变化的影响.结果表明:(1)横坡垄作相对于顺坡垄作能显著减少径流总量97.1％...     

虎源流感病毒RT-PCR检测方法的建立及其应用研究

为临床上能快速诊断虎流感,通过对已分离获得的虎源H5N1流感病毒和GenBank中报道的A型H5N1亚型流感病毒的NP、HA、NA序列,设计合成各个基因的特异性PCR扩增引物和A型流感病毒通用反转录引物。通过优化反应体系及条件,建立一步法可同时扩增出3个基因的联合RT-PCR检测方法。将该方法用于临床病料检测,并与电镜负染、HA/HI及病毒分离等方法进行比较。结果,NP、HA、NA基因的扩增片段大小分别为464bp、581bp、173bp,其检测的敏感性约100个TCID50。该方法的检出率和病毒分离结果相符,高于电镜负染和HA/HI试验。结果显示,该方法可用于虎源流感病毒的临床或实验室检测。     

西藏种质资源的保护与可持续利用

通过对西藏种养业种质资源情况、保护利用现状和存在问题进行系统分析,并梳理了西藏种质资源保护与可持续利用的基本思路和重点建设内容,结果表明,西藏种养业种质资源具有自身的特色和优势,良种繁育体系建设取得了明显成效,但存在品种退化、混杂严重,经营体制尚不健全,基础设施薄弱,良种繁育任务重、推广普及难度大等问题;下一步应着重围绕提高种养业良种生产能力、质量水平和增强农牧产品市场竞争力的发展目标,建立具有西藏特色的新型良种繁育体系,重点完善农作物、畜禽、水产三大良种工程;同时提出了保障措施,包括加强领导,提高认识;多元投入,确保建设资金到位;依靠科技进步,提高育种水平;加大扶持重点良种企业,推动良种产业化;深化改革,加强良种管理。     

带棒青贮饲用玉米在奶牛日粮中的增产效果

以带棒青贮饲用玉米代替青贮玉米秸秆饲喂泌乳奶牛,30d内头日平均增产奶量6.62kg,资金投入产出比为1:4.5。以玉米作带棒青贮比用作生产玉米子粒亩增收120元,推广玉米带棒青贮是提高粮农和奶农经济收入的重要途径。     

陕西省兰科植物1个新记录属——蛤兰属

本文报道了属于陕西省兰科植物分布新记录的1属——蛤兰属Conchidium Griffith,和陕西省分布新记录种高山蛤兰Conchidium japonicum(Maxim.)S.C.Chen et J.J.Wood,迄今为止,蛤兰属和高山蛤兰在陕西省只发现于大巴山地区的镇坪县。