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91.
Following growth in a chemically defined medium (CDM), five strains of Streptococcus uberis were tested for their ability to survive killing by bovine neutrophils. Strains 0140J, ST10, EF20 and C221 were easily killed, whereas strain C197C was highly resistant. The ability of strain 0140J to resist phagocytosis and killing was increased by supplementation of the growth medium with milk whey, casaminoacids, casein, or, to a lesser extent, bovine serum. Supplementation of the growth medium with yeast extract or bovine serum albumin did not affect the resistance of this strain. Following growth in CDM supplemented with casein, strains ST10 and C221, like strain 0140J, were significantly more resistant to killing by neutrophils. The resistance of strains EF20 and C197C was unaffected by the addition of casein to the medium; strain EF20 remained susceptible and strain C197C highly resistant to killing. The effect of supplementing the growth media with components other than casein was only studied for strain 0140J. Decapsulation of strains C197C, ST10 and 0140J, grown in CDM + casein, with type-X hyaluronidase did not significantly affect their ability to survive in the presence of bovine neutrophils. 相似文献
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Current theories hypothesize that dopamine neuronal firing encodes reward prediction errors. Although studies in nonhuman species provide direct support for this theory, functional magnetic resonance imaging (fMRI) studies in humans have focused on brain areas targeted by dopamine neurons [ventral striatum (VStr)] rather than on brainstem dopaminergic nuclei [ventral tegmental area (VTA) and substantia nigra]. We used fMRI tailored to directly image the brainstem. When primary rewards were used in an experiment, the VTA blood oxygen level-dependent (BOLD) response reflected a positive reward prediction error, whereas the VStr encoded positive and negative reward prediction errors. When monetary gains and losses were used, VTA BOLD responses reflected positive reward prediction errors modulated by the probability of winning. We detected no significant VTA BOLD response to nonrewarding events. 相似文献
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Kate L. Tsai Karen M. Vernau Kathryn Winger Danielle M. Zwueste Beverly K. Sturges Marguerite Knipe D. Colette Williams Kendall J. Anderson Jacquelyn M. Evans Ling T. Guo Leigh Anne Clark G. Diane Shelton 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(1):258-265
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Fiona J. Leigh Ian Mackay Hugo R. Oliveira Nicholas E. Gosman Richard A. Horsnell Huw Jones Jon White Wayne Powell Terence A. Brown 《Genetic Resources and Crop Evolution》2013,60(6):1831-1842
Chloroplast microsatellites (SSRs) are conserved within wheat species, yet are sufficiently polymorphic between and within species to be useful for evolutionary studies. This study describes the relationships among a very large set of accessions of Triticum urartu Thum. ex Gandil., T. dicoccoides (Körn. ex Asch. et Graebn.) Schweinf., T. dicoccon Schrank, T. durum Desf., T. spelta L., and T. aestivum L. s. str. based on their cpSSR genotypes. By characterising the chloroplast diversity in each wheat species in the evolutionary series, the impact on diversity of major evolutionary events such as domestication and polyploidyisation was assessed. We detected bottlenecks associated with domestication, polyploidisation and selection, yet these constrictions were partially offset by mutations in the chloroplast SSR loci that generated new alleles. The discrete cpSSR alleles and haplotypes observed in T. urartu and Aegilops tauschii, combined with other species specific polymorphisms, provide very strong evidence that concur with current opinion that neither species was the maternal and thus cytoplasmic donor for polyploid wheats. Synthetic hexaploid wheats possessed the same chloroplast haplotypes as their tetraploid progenitors demonstrating how the novel synthetic wheat lines have captured chloroplast diversity from the maternal parents, the chloroplast is maternally inherited and novel alleles are not created by genomic rearrangements triggered by the polyploidisation event. 相似文献
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Antimicrobial resistance of fecal Escherichia coli isolated from grow-finish pigs in 20 herds in Alberta and Saskatchewan. 下载免费PDF全文
Leigh B. Rosengren Cheryl L. Waldner Richard J. Reid-Smith Sylvia L. Checkley Margaret E. McFall Andrijana Raji? 《Canadian journal of veterinary research》2008,72(2):160-167
Escherichia coli (n = 1439), isolated from the feces of apparently healthy grow-finish pigs in 20 herds in Alberta and Saskatchewan, were tested for susceptibility to 16 antimicrobials. All isolates were susceptible to amikacin, ceftriaxone, and ciprofloxacin and less than 1% was resistant to amoxicillin-clavulanic acid, cefoxitin, ceftiofur, gentamicin, and nalidixic acid. Resistance was most common to tetracycline (66.8%), sulfamethoxazole (46.0%) and streptomycin (33.4%). Twenty-one percent of the isolates were susceptible to all drugs, while 57% were resistant to 2 or more antimicrobials. Unconditional associations between resistances provided insight into the potential for co-selection. Every resistance-outcome was associated with at least 2 other drug-resistances. These associations illustrate the propensity for resistance phenotypes to occur together and the importance of considering co-selection in antimicrobial use decisions. A 2nd analysis explored the associations between resistance phenotypes in E. coli and Salmonella spp. from the same herd. Only 2 resistances in Salmonella were associated with herd-level E. coli resistance, indicating that E. coli is a poor sentinel for Salmonella AMR within herds. Herd-level management, including antimicrobial use, could affect antimicrobial resistance. The intra-class correlation between isolates within herds ranged from 0.1 to 0.46, which confirmed resistance clustered within herds. This suggests herd-level interventions might mitigate antimicrobial resistance. Overall, these results reflect the on-farm selection pressure for resistance and the potential food-safety risk from near-market animals. These data provide a baseline for comparisons with future on-farm monitoring of antimicrobial resistance in E. coli. 相似文献
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The notion that a trade-off exists between immunity and reproduction is now a central concept in theories of sexual selection. However, whether such a trade-off exists between immunity and gamete viability has not been established. Here we show that genetic variance for high levels of an immune response required to fight bacterial infections is associated with genetic variance for low sperm viability. These data have implications for our understanding of sexual selection mechanisms and of reproductive costs in male longevity. 相似文献