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951.
Dietary α‐linolenic acid affects lipid metabolism and tissue fatty acid profile and induces apoptosis in intraperitoneal adipose tissue of juvenile grass carp (Ctenopharyngodon idella) 下载免费PDF全文
A 56‐day feeding trial was conducted to elucidate the effects and mechanism action of dietary α‐linolenic acid (ALA, 18:3n‐3) on lipid accumulation and fatty acid profile of muscle, hepatopancreas and intraperitoneal fat (IPF) in juvenile grass carp using three isonitrogenous and isoenergetic semi‐purified diets containing 0.0% (control group), 1.0% and 2.0% ALA, respectively. The lowest intraperitoneal fat (IPF) ratio was found in 2.0% group. In the muscle, hepatopancreas and IPF, docosahexaenoic acid (DHA, 22:6n‐3) and eicosapentaenoic acid (EPA, 20:5n‐3) contents increased with the increase in dietary ALA. In the IPF, caspase 3, caspase 8 and caspase 9 showed the highest activities in 2.0% group, while the value of Bcl‐2/Bax (B‐cell leukaemia 2/Bcl‐2‐associated X protein) reached the lowest. Meanwhile, swelling of the IPF mitochondria was observed in 2.0% group. The gene expressions of fatty acid desaturase (FAD) and fatty acid elongase (ELO) in the hepatopancreas and muscle showed significantly higher levels in the treatment groups, whereas an opposite trend was existed in the IPF. Fatty acid synthase (FAS), sterol regulatory element binding protein‐1c (SREBP‐1c) in the IPF and hepatopancreas reached the lowest in 2.0% group. Overall, dietary ALA could promote n‐3 highly unsaturated fatty acids (HUFAs) synthesis and suppress the accumulation of lipid by decreasing the expression of related genes and promoting the apoptosis in IPF. 相似文献
952.
工厂化养殖仿刺参营养品质分析与评价 总被引:1,自引:0,他引:1
为了更好地了解工厂化养殖仿刺参的营养成分和品质,本研究采用国标等方法,分别对工厂化养殖、池塘养殖、底播自然生长和海捕野生仿刺参的各项营养成分进行了分析和评价。结果显示,工厂化养殖仿刺参出皮率为62.7%~65.8%,煮后出皮率为24.8%~31.1%,显著高于其他来源仿刺参。工厂化养殖仿刺参的必需氨基酸/非必需氨基酸为0.46~0.50,氨基酸营养价值平均得分为87.89~90.42,均高于其他来源仿刺参,说明其氨基酸营养价值水平较高。在其他营养成分方面,工厂化养殖仿刺参与池塘养殖仿刺参较为相近。自然环境生长仿刺参由于摄食来源广泛、生长周期较长,其蛋白质与脂肪水平普遍高于其他来源仿刺参。综合分析认为,工厂化养殖仿刺参的营养价值与池塘养殖仿刺参相近,在出皮率和氨基酸营养水平上优于池塘养殖和自然环境生长仿刺参,说明工厂化养殖仿刺参具有较好的品质和营养价值。 相似文献
953.
齐口裂腹鱼幼鱼对蛋白质、脂肪和碳水化合物需要量研究 总被引:1,自引:0,他引:1
试验设21个日粮组,蛋白质、脂肪、碳水化合物梯度变化范围分别在33%~51%、3%~18%、23%~32%.在水温(25.25±1.91)℃条件下,在水泥池中对平均体苇为(2.01±0.04)g的360尾齐口裂腹鱼进行了60 d的生长试验,研究日粮中蛋白质、脂肪和碳水化合物对齐口裂腹龟生长、体组成和肝脏组织的影响.结果表明:各组鱼特定生长率随着日粮蛋白质水平的增加而增加;体蛋白含量不受日粮组成的影响,体脂肪随着日粮脂肪水平的增加而增加,体碳水化合物受日粮碳水化合物的影响极显著,而不受日粮蛋白质和脂肪的影响.齐口裂腹鱼幼鱼能耐忍23%~26%的碳水化合物水平,且能利用6%~12%的日粮脂肪.基于齐口裂腹鱼幼鱼对只粮碳水化合物和脂肪含量需求分别为23%~26%和6%~12%时,蛋门质需求量为42%~48%,能蛋比为41.3 kJ/g. 相似文献
954.
955.
Bermudagrass (Cynodon ssp.) germplasm is genetically diverse and widely distributed in the world. The study was conducted to identify and assess the molecular variation and relationship among 24 cultivars developed in China, Australia and the USA. Sequence-Related Amplified Polymorphism (SRAP) was applied to cultivars identification in this study for the first time. Thirty of the 90 SRAP primer combinations generated a total of 274 clearly bands encompassing 249 (91%) polymorphic. Each bermudagrass cultivar has its unique binary code and can be distinguished from the others. Three distinct clusters were obtained by unweighted pair-group method with arithmetic averages based on the polymorphic markers. Coefficients of genetic distance among the genotypes ranged from 0.57 to 0.97. The results demonstrated that SRAP marker is a stable molecular marker technique for the identification of bermudagrass cultivars and their genetic relationships. 相似文献
956.
AIM: To investigate the mechanisms of transdifferentiation of renal tubular epithelial cells by the observation of serial activation of embryonic pax2 and WT1 genes in chronic renal failure model of 5/6 nephrectomized injury. METHODS: Embryo of mice, cultured renal tubule cells and chronic renal failure rat model of 5/6 nephrectomized injury were established. The expressions of paired Box gene (pax2) and Willm’s tumor gene (WT1), as well as α-smooth muscle actin (α-SMA), a phenotype of mesenchymal cells, were detected by RT-PCR and immunohistochemistry techniques. RESULTS: (1) Expressions of pax2 and WT1 mRNA began at 11.5 d and 14 d of embryo and increased gradually, and expression in trace quantity at 2 weeks after birth. Immunohistochemistry analysis revealed that WT1 only expressed in the glomerular podocytes and expressions of pax2 and WT1 in adult renal tubular cells were negative. (2) Deno-expression of pax2 and WT1 in some tubular cells appeared at week 2 and peaked at week 4 in 5/6 nephrectomized rats, both showing a same trend of expression. However, pax2 showed another peak at week 10 afterwards. (3) Re-expressions of pax2 and WT1 in TEC at 0.5 and 24 h after treated with IL-1α (10 μg/L) or AngII (10-9 mol/L) were observed respectively, followed by upregulation of α-SMA expression, and mesenchymal cells characters were shown. The effects were inhibited by Ang II receptor 2 antagonist and WT1 antibody, respectively. CONCLUSION: Adult renal tubule cells acquire re-activation of embryonic pax2 and WT1 genes and phenotypes of mesenchymes when challenge with certain injuries. Deno-expression of pax2 and WT1 genes closely relates with high concentration of bioactive facors, such as AngII and IL-1, which are involved in the mechanisms of renal tubule cells transdifferentiation. 相似文献
957.
958.
河北省委省政府、省农业厅高度重视农产品质量安全问题,多次部署开展我省农业、畜牧业,水产业产品的质量安全整治行动。各地方政府、各有关部门做了大量工作,取得了可喜成绩。我省在农业部抽查的水产品中,合格率接近100%,取得认证的养殖基地、良种场都能够按要求操作,建立了生产用药和饲料记录、销售记录、生产规程等,并对产品定期检测。 相似文献
959.
960.