全文获取类型
收费全文 | 37247篇 |
免费 | 1299篇 |
国内免费 | 2329篇 |
专业分类
林业 | 5569篇 |
农学 | 3575篇 |
基础科学 | 1807篇 |
5745篇 | |
综合类 | 8837篇 |
农作物 | 3372篇 |
水产渔业 | 2624篇 |
畜牧兽医 | 4059篇 |
园艺 | 2178篇 |
植物保护 | 3109篇 |
出版年
2024年 | 84篇 |
2023年 | 355篇 |
2022年 | 922篇 |
2021年 | 1080篇 |
2020年 | 1059篇 |
2019年 | 942篇 |
2018年 | 3399篇 |
2017年 | 3643篇 |
2016年 | 1990篇 |
2015年 | 1177篇 |
2014年 | 1197篇 |
2013年 | 1318篇 |
2012年 | 2473篇 |
2011年 | 3933篇 |
2010年 | 3765篇 |
2009年 | 2722篇 |
2008年 | 2637篇 |
2007年 | 2781篇 |
2006年 | 1013篇 |
2005年 | 875篇 |
2004年 | 539篇 |
2003年 | 423篇 |
2002年 | 348篇 |
2001年 | 280篇 |
2000年 | 298篇 |
1999年 | 244篇 |
1998年 | 180篇 |
1997年 | 178篇 |
1996年 | 187篇 |
1995年 | 142篇 |
1994年 | 115篇 |
1993年 | 125篇 |
1992年 | 114篇 |
1991年 | 69篇 |
1990年 | 53篇 |
1989年 | 51篇 |
1988年 | 60篇 |
1987年 | 31篇 |
1986年 | 10篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 9篇 |
1977年 | 4篇 |
1968年 | 4篇 |
1963年 | 3篇 |
1962年 | 2篇 |
1956年 | 7篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
72.
优氯净对苏云金杆菌伴孢晶体消毒效果及机理的研究 总被引:1,自引:1,他引:0
苏云金杆菌(Bacillus thuringiensis,简称BT菌)的伴孢晶体被优氯净液处理后,在相差显微镜下观察,失去暗蓝色光泽,呈暗黑色,难溶于碱性缓冲液。虽能溶于昆虫的胃液,却失去致病性。用扫描电镜和SDS—聚丙烯酰胺凝胶电泳进一步研究,结果表明:优氯净能破坏伴孢晶体蛋白的一级和立体结构,使晶体蛋白失活,达到消毒的目的。消毒能力测定结果表明:1毫升0.03%有效氯优氯净液在3分钟内能消毒50微升BT菌液(3μg伴孢晶体/μl菌液)。消毒BT菌污染桑叶,用0.01%有效氯优氯净液浸泡3分钟即可。考虑到生产上的安全性,我们建议用0.05%有效氯优氯净消毒3—5分钟。 相似文献
73.
74.
75.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
76.
嫁接对茄子果实中蛋白质、VC、可溶性糖含量及分布的影响 总被引:4,自引:0,他引:4
以托鲁巴姆为砧木、西安绿茄为接穗进行嫁接,研究嫁接对茄子果实中蛋白质、VC、可溶性糖含量及分布的影响。结果表明,嫁接后除可溶性糖含量略低于对照外,蛋白质、VC及果实中的含水量均高于对照。嫁接苗果实中主要营养成分在果实各部位的分布规律基本不变,内果皮中各种养分的含量均高于外果皮,由果实顶部至蒂部各种养分的变化规律不尽相同。随着果实的膨大,嫁接苗果实中主要营养成分的变化规律与对照基本一致。瞪眼期果实中的蛋白质含量较高,进入商品成熟期后,果实中的养分含量基本不变,趋于稳定。 相似文献
77.
苜蓿育种几个问题的探讨 总被引:6,自引:0,他引:6
在对主要苜蓿遗传资源与遗传特性进行陈述的同时, 重点对苜蓿遗传特性与育种进程的关系、目前常见的育种方法以及苜蓿育种的发展现状进行了阐述,并就近年来我国在苜蓿育种领域内存在的主要问题进行了分析,旨在为今后的苜蓿育种工作提供参考依据。 相似文献
78.
本研究采用A ndersen-6级空气微生物样品收集器,选用血-葡萄糖-琼脂培养基为采样介质,对两个不同种兔舍环境空气中需氧菌总数和葡萄球菌总数进行了检测,并对葡萄球菌的菌群组成进行了分析。结果表明,两个兔舍内需氧菌含量分别为1.73~85.8×103CFU/m3、2.71~9.66×103CFU/m3空气,葡萄球菌含量分别为0.94~7.84×103CFU/m3、1.02~6.54×103CFU/m3空气。兔舍空气中葡萄球菌主要包括金黄色葡萄球菌、腐生葡萄球菌、表皮葡萄球菌、科氏葡萄球菌、头状葡萄球菌和马胃葡萄球菌,其中金黄色葡萄球菌的含量占葡萄球菌总数的26.3%~29.6%,其次是腐生葡萄球菌和表皮葡萄球菌。另外,还对需氧菌和金黄色葡萄球菌在A ndersen-6级收集器不同层级上的分布情况进行了统计分析,结果表明,约有56.4%的需氧菌和49%金黄色葡萄球菌分布在3~6层上,空气动力学直径(A erody-nam ic d iam eter,D ae)在6~0.2μm,它们能进入人、畜的气管、支气管,甚至细支气管,对饲养员和动物的呼吸道构成严重危害。 相似文献
79.
AIM: To study the effect of cocaine on caspase-3 in myocardiac cells of male rats in different age. METHODS: Three-week-old(n=16), six-week-old(n=16) and twelve-week-old (n=16) male Sprague-Dawley rats were all divided into control groups and experiment groups randomly, each group had eight animals, experiment groups were given cocaine hydrochloride (15 mg·kg-1 body weight) subcutaneously daily for four weeks. The ratio of heart weight to body weight (HW/BW, mg/g) were measured. DNA fragmentation of myocardia cells was determined by gel electrophoresis, and caspase-3 activity in myocardia cells was tested by flow cytometry (FCM). RESULTS: In three experiment groups, the DNA isolated from myocardial cells displayed clear ladder pattern. The HW/BW and the caspase-3 activity were increased significantly than those of control groups (P<0.01). CONCLUSIONS: Cocaine induced apoptosis in rat myocardial cells. The increase in caspase-3 activity may be the one of important pathways related to the induction of apoptosis. 相似文献
80.
Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献