Effects of Rumen-Protected Arginine and Soybean Oil on Intramuscular Fat Content and Fatty Acid Composition in Fine Wool Sheep

本试验旨在研究饲粮添加不同水平的过瘤胃保护性精氨酸( rumen-protected arginine,RP-Arg)和大豆油对细毛羊肌内脂肪和脂肪酸组成的影响.试验选用健康、体重相近的15只细毛羊,随机分为5组,对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加1.5 g/d RP-Arg、2.0 g/d RP-Arg、1.5 g/d RP-Arg+3％大豆油和2.0 g/d RP-Arg+3％大豆油的饲粮.预试期为5d,正试期为45 d.试验结束后每组选2只羊进行屠宰,取背最长肌样用于测定肉质指标和脂肪酸组成.结果表明:试验组肌肉pH、系水率和熟肉率与对照组差异不显著(P＞0.05),而肌内脂肪含量显著高于对照组(P＜0.05);试验组肌肉多不饱和脂肪酸含量与对照组相比显著提高(P＜0.05);添加RP-Arg和大豆油有提高肌肉过氧化物酶体增生物激活受体γ(PPARγ)、脂蛋白脂酶(LPL) mRNA表达量,降低激素敏感性甘油三酯脂肪酶(HSL) mRNA表达量的趋势.综合指标,以1.5 g/d RP-Arg组与1.5 g/d RP-Arg+3％大豆油组添加效果较好.     

抗猫泛白细胞减少症病毒单克隆抗体的制备及其生物学特性鉴定

用 PEG60 0 0沉淀和蔗糖密度梯度离心从细胞培养物中纯化猫泛白细胞减少症病毒 ( FPV) ,以纯化FPV免疫 BALB/c小鼠 ,运用淋巴细胞杂交瘤技术 ,获得了 4株抗 FPV的特异性单克隆抗体 ( Mc Ab)。其腹水 Mc Ab的 ELISA效价在 1 0 - 4～ 1 0 - 5之间 ,其中 1株具有血凝抑制能力。经 ELISA阻断试验及 ELISA交叉反应性试验测定 ,这 4株 Mc Ab可与 FPV、犬细小病毒 ( CPV)和水貂肠炎病毒 ( MEV)呈特异性反应 ,因此可作为检测 FPV、CPV和 MEV共同抗原的通用试剂     

兴安盟地区旱作条件下11个苜蓿品种生产性能和饲用价值比较

为筛选兴安盟地区旱作条件下适宜引进栽培的优良苜蓿品种,在兴安盟乌兰浩特市呼和马场种植11个苜蓿(Medicago sativa)品种,通过连续2年观测参试品种的越冬率、株高、产量、茎叶比及营养成分等指标,对旱作条件下11个苜蓿品种的生产性能和饲用价值进行灰色关联分析.结果表明:图牧2号、敖汉苜蓿和呼伦贝尔杂花苜蓿两年的...     

尼卡巴嗪和氨丙啉单独及配合应用对鸡球虫病的疗效

目的研究尼卡巴嗪和氨丙啉单独及配合应用对鸡球虫病的疗效。方法将15日龄鸡人工感染柔嫩艾美耳球虫,以鸡平均增重率、盲肠病变记分、粪便记分和抗球虫指数等指标为参数,综合评价尼卡巴嗪和氨丙啉单独及配合应用的抗球虫效果。结果尼卡巴嗪左旋咪唑 大蒜素 维生素K组抗球虫指数为189。氨丙啉 乙氧酰胺甲酯 乙胺嘧啶 磺胺喹啉组抗球虫指数为184.5,这两组药均具有良好的抗球虫效果。氨丙啉 乙氧酰胺苯甲酯组,氨丙啉 乙胺嘧啶组及氨丙啉药物对照组抗球虫指数分别为162.8,163.2,和160.5;尼卡巴嗪 乙氧酰胺苯甲酯组和单用尼卡巴嗪组抗球虫疗效较差,AC I分别为142.7和146.4。结论尼卡巴嗪配合左旋咪唑、大蒜和维生素K产生了增效作用;用氨丙啉配合乙氧酰胺苯甲酯、乙胺嘧啶和磺胺喹啉也可达到增效目的,经两药单用提高了疗效。     

稻纵卷叶螟控制方法研究进展

近年来,稻纵卷叶螟已成为害水稻的重要害虫。结合生产实际,介绍稻纵卷叶螟的发生、分布、危害情况,重点讨论了控制稻纵卷叶螟发生的新方法和技术,指出存在的问题及对策。     

塔里木河干流河道生态需水量研究

基于有序聚类法,在对1957-2006年塔里木河干流径流序列受人类活动显著影响突变点判别的基础上,对干流新渠满、乌斯满和恰拉3个水文断面河道逐月最小生态需水量和适宜需水量进行计算。结果表明：① 近50 a来,人类活动对干流水资源影响程度不断增大,1976年前的径流量可以近似看作自然状态;② 新渠满、乌斯满和恰拉河道年最小需水量分别为17.68×10⁸ m³、10.02×10⁸ m³和3.29×10⁸ m³,年适宜需水量分别为35.65×10⁸ m³、18.23×10⁸ m³和7.00×10⁸ m³;③一般用水期（10月至翌年3月）保证新渠满、乌斯满和恰拉河道最小需水量5.33×10⁸ m³、3.77×10⁸ m³和1.32×10⁸ m³,用水敏感期（4-9月）保证适宜需水量28.99×10⁸ m³、13.70×10⁸ m³和5.09×10⁸ m³即可确保干流河道生态系统健康。     

Mechanism of exogenous nitric oxide in attenuating endotoxin-induced increase of rat pulmonary microvascular permeability

AIM and METHODS:The animal model of acute lung injury (ALI) caused by intratracheal instillation of lipopolysaccharides(LPS) in vivo and human peripheral blood polymorphonuclear neutrophil (PMN) in vitro were used to study the effects of sodium nitroprusside (SNP), nitric oxide (NO) donor, on LPS-induced PMN accumulation, microvascular permeability and PMN apoptosis. RESULTS:①In vivo, PMN accumulation in lung, the protein content in bronchoalveolar lavage fluid (BALF) and the Evans blue dye and monastral blue dye extravasation in lung tissue of LPS group were markedly higher than those of both sham operation group and LPS+SNP group. ②In vitro, the apoptotic percentage of SNP group was much higher than that of control group, while compared with LPS group, SNP+LPS group has significantly higher apoptotic percentage. CONCLUSIONS:SNP intratracheal instillation attenuated LPS-induced microvascular permeability and alleviated ALI. PMN apoptosis induced by SNP may be one of the potential mechanisms underlying the decrease of PMN accumulation in lung tissue.     

红壤甜橙园土壤和叶片营养元素相关性分析

 于2010 年和2011 年在江西省安远县和寻乌县这两大赣南甜橙生产县,共采集181 个红壤枳 砧甜橙园的土壤样品和叶片样品,进行营养元素含量测定,并对土壤和叶片相应营养元素含量进行相关 性分析,为评价测土配方施肥是否适宜红壤甜橙园提供依据。结果表明：土壤碱解氮、交换性钙、交换 性镁、有效铁和有效锌的丰缺状况在甜橙叶片对应营养元素丰缺水平上能得到一定程度的反映;土壤有 效钾、有效磷、有效铜和有效硼的状况则难以在甜橙叶片对应营养元素丰缺水平上得到反映。相关性分 析结果显示,只有2010 年安远县甜橙园土壤有效Zn 含量与叶片Zn 含量呈显著正相关（P < 0.05）,其余 土壤有效营养元素含量与叶片对应营养元素含量之间均无显著相关性。表明测土配方施肥不适宜红壤甜 橙园,建议实行以叶片营养诊断为主、土壤诊断为辅的配方施肥策略。     

Role of TLR4-TRPC6 in LPS-induced NF-κB P65 expression and nuclear translocation in airway epithelial 16HBE cells

AIM: To investigate the role of Toll-like receptor 4 (TLR4) and transient receptor potential channel 6 (TRPC6) signaling pathway in lipopolysaccharide (LPS)-induced nuclear factor-κB (NF-κB) P65 expression and nuclear translocation in airway epithelial cells (16HBE) for supplementing the mechanism for airway inflammation. METHODS: After stimulating the 16HBE cells with LPS at 1 mg/L for 0, 0.5, 2, 6, 12 and 24 h, the expression of NF-κB P65 at mRNA and protein levels in the 16HBE cells were determined by RT-PCR and Western blot respectively, and the nuclear translocation of NF-κB P65 was detected by immunocytochemical staining method. The effects of TLR4 inhibitor CLI-095 at 5 μmol/L and TRPC6 agonist Hyp9 at 10 μmol/L on LPS (1 mg/L)-induced NF-κB P65 expression and nuclear translocation in the 16HBE cells were determined by RT-PCR, Western blot and immunocytochemical staining. RESULTS: LPS increased the mRNA and protein expression of NF-κB P65 and nuclear translocation in the 16HBE cells(P<0.05). TLR4 inhibitor CLI-095 reduced the mRNA and protein expression of NF-κB P65 and nuclear translocation induced by LPS, while Hyp9 enhanced the mRNA and protein expression of NF-κB P65 and nuclear translocation induced by LPS in the 16HBE cells(P<0.05). CONCLUSION: LPS induces the expression and nuclear translocation of NF-κB P65 in the 16HBE cells via TLR4-TRPC6 signaling pathway.     

Effect of antifibrotic herb serum on rat hepatic stellate cell proliferation and collagen synthesis

AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[³H]H]proline and [[³H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P＜0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P＜0.05). Portal serum decreasea collagen synthesis (P＜0.05), but circumferential serum had no effect (P＞0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action.