叶菜型甘薯大棚越冬栽培薯苗存活率及其产量性状表现

为提高叶菜型甘薯的繁殖系数,保证种植种源的持续供应,采用薯苗大棚栽培越冬留种,研究不同品种和节位薯苗越冬栽培的薯苗地上和地下部性状。结果表明:叶菜型甘薯福菜薯18和鄂菜薯1号的茎尖薯苗和二节薯苗越冬存活率最高为77.50%,最低为66.25%;鄂菜薯1号茎尖薯苗越冬苗株高最高为29.3cm,茎尖薯苗株高均与二节薯苗的株高差异显著;二节薯苗分枝数、薯苗产量最高分别为62.77个/m2、493.34kg/667m2,均显著高于茎尖薯苗;越冬地下茎存活率以二节薯苗优于茎尖薯苗,存活率在73.75%~82.50%;茎尖薯苗的柴根产量和数量均优于二节薯苗,分别达128.89kg/667m2和16.1个/m2。结论:以二节薯苗栽培越冬,其来年薯苗分枝、产量、地下茎性状均优于茎尖薯苗,薯苗直接栽插繁殖、地下茎萌发繁殖速度均快于柴根。     

两个新的黄瓜SnRK2基因的鉴定及特征分析

植物SnRK2基因在非生物逆境应答中具有重要功能,但有关黄瓜SnRK2基因的研究尚缺乏报道。笔者通过对黄瓜基因组的重新挖掘,发现了2个新的SnRK2基因,分别与拟南芥的SnRK2.4和SnRK2.10同源。本试验对2个新的黄瓜SnRK2基因的结构特征及顺式调控元件进行了全面分析,为进一步研究其在黄瓜逆境应答中的功能奠定了基础。     

基于加权Voronoi图的林木竞争指数

以福寿山林场25年生的杉木生态公益林为研究对象,通过分析林木竞争指数与胸径生长因子的相关关系,得出二者为负相关,从而确定加权Voronoi图的权重为胸径倒数(W),借助Arcgis软件里的加权Voronoi图工具,通过加权Voronoi图的方法来确定竞争单元,提出基于加权Voronoi图的W_V_Hegyi竞争指数,最后将W_V_Hegyi竞争指数与Hegyi、V_Hegyi竞争指数进行对比分析,结果表明:1) 3种竞争指数与胸径相关性强弱排序为:W_V_HegyiV_HegyiHegyi;2) 3种竞争指数与胸径服从幂函数关系,曲线拟合度排序为:W_V_HegyiV_HegyiHegyi;3) 样地竞争指数平均值排序均为:V_HegyiW_V_HegyiHegyi。通过以上比较分析证明,W_V_Hegyi竞争指数比Hegyi、V_Hegyi竞争指数能更准确地反映林木间的竞争关系。      

以彝人古镇为例论我国新兴旅游形式的发展

旅游是社会生产力发展到一定阶段的产物,是人们在满足了基本生活需要的基础上逐渐所形成的更高层次的消费需求,随着经济的发展以及社会不断的进步,人们思维方式的不断转变对于旅游的要求也逐渐发生了转变。     

响应面优化丁烷-亚临界法制取桐油技术的研究

桐油是一种重要用途的干性油脂,不同工艺会对油品质量产生重要影响。为了克服传统工艺在制油中的缺陷,本研究采用丁烷-亚临界法工艺进行桐油的制备,并基于Design-Expert软件进行响应面优化实验,获得最佳工艺参数:萃取温度40.64℃,萃取时间45.38 min,原料颗粒度39.71目,在此条件下桐油得油率达到66.12%。采用气质联用仪分析了桐油脂肪酸组成为α-桐酸74.93%,亚油酸8.62%,油酸7.12%,棕榈酸2.72%,硬脂酸3.85%,通过分析所得油样的5项指标,可知该工艺所得油品满足国标要求。     

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.     

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.     

不同种源软枣猕猴桃幼苗光合特性的比较及变异分析

以5个种源的软枣猕猴桃2年生幼苗为试材,采用LI-6400便携式光合测定系统测定其光合指标,分析不同种源软枣猕猴桃的光合日变化,揭示种源间光合特性的差异。结果表明:不同种源软枣猕猴桃幼苗净光合速率、气孔导度和水分利用效率日变化呈"双峰"曲线;蒸腾速率有"单峰型"和"双峰型"2种形式;胞间CO_2浓度呈‘W’型趋势;不同种源间存在一定的差异,各时间段的光合指标大小、峰值的大小及出现的时间有所不同。不同种源间的净光合速率、蒸腾速率、气孔导度、胞间CO_2浓度和水分利用效率存在极显著差异,通过隶属函数值综合评价,敦化和林口种源的光合能力强,属于较高光效种源。     

不同播期对鲁原502小麦产量的影响

为实现良种良法配套推广应用、大面积高产,以目前莒县优选的鲁原502小麦作供试材料,对该品种的播期进行研究。结果表明,播期对鲁原502产量的影响显著,其在莒县的适宜播期为10月5—15日。     

不同滴灌频率对大田覆膜小西瓜生长特性及品质的影响

在灌溉定额一定的情况下,研究了不同滴灌频率对大田膜下小西瓜在果实膨大期以及成熟期内有关特性的影响,以期为豫北平原地区大田膜下滴灌小西瓜生长中后期阶段确定最佳的滴灌频率。结果表明:不同灌溉频率对小西瓜生长过程中根粗、茎粗和蔓长的影响不是特别明显。从试验数据来看,以果实膨大期中频(T3),果实成熟期低频(T5)处理效果较好。此灌溉频率组合下不仅小西瓜长势好,而且甜度较大,省工省时又节水。