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61.
AbstractWith the deepening of economic reform,China's oil companies have basical y established a modern enterprise financial management system, mode of operation and management have had a fundamental c...  相似文献   
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Biodegradation kinetics of dicofol by selected microorganisms   总被引:1,自引:0,他引:1  
The capacity of some plant growth promoters (seven species of bacteria namely, Azospirillium barasilense, Azotobacter chroococcum, Klebsilense pneumoneae, Pseudomonas cepacia, Bacillus subtilis, Pseudomonas fluorescens and Bacillus polymyxa) and some control agents for plant pathogens (two species of fungi namely, Trichoderma viride and Trichoderma harzianum) to degrade dicofol pesticide (DCF) in liquid culture media was investigated. The recovered amount of DCF was extracted based on the solid phase extraction (SPE) with methylene chloride and then analyzed by HPLC. Contrary to published reports, no intermediate or final degradation metabolites of DCF could be observed. About 26-33% of DCF degradation was observed after 3 days of incubation with the tested bacteria which increased to 61-80%, 74-85%, 77-87% and 75-94% after 7, 14, 21 and 28 days of incubation, respectively. On the other hand, the tested fungi removed roughly 35% of available DCF within the first three days of incubation which increased to 84-87%, 89-95%, 91-95% and 92-96% after 7, 14, 21 and 28 days of incubation, respectively. A biphasic model was assumed to explore the disappearance of DCF from media enriched with either bacteria or fungi, where the rate of disappearance in the first phase was faster than the second. This is clearly reflected in the half-life (t1/2) for the first and second phases, where the t1/2 values of DCF ranged from 2.82-4.42 to 19.32-29.73 days, respectively. The results have implications for the development of a bioremediation strategy.  相似文献   
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Recent advances in next generation sequencing technologies make genotyping-by-sequencing (GBS) more feasible for molecular characterization of plant germplasm with complex and unsequenced genomes. Here we applied the GBS technology to assess the genetic diversity of 275 hexaploid oat wild relative (Avena sterilis) plants collected from 24 natural populations in Jordan. Total genomic DNAs were extracted and digested with restriction enzymes PstI and MspI. Three Illumina MiSeq sequencing runs generated 556 paired-end FASTQ files with 127,128,438 raw sequences. Bioinformatics analysis identified an informative matrix of 275 samples × 12,999 SNP markers. Analysis revealed 52.4% of SNP variation residing among 24 populations and eight major genetic clusters of the samples. Most samples were grouped together within their original populations. A significant association of pairwise population genetic distances was found with latitudinal or longitudinal differences. Two natural populations were highly differentiated from the others, and 30 highly distinct A. sterilis samples were identified from seven populations. These findings are useful for understanding genetic variability and conservation of natural A. sterilis populations, and they demonstrate the advances of the GBS application for germplasm characterization of crop wild relatives with complex genomes.  相似文献   
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Thirty seven cases of bursitis presented to our Veterinary Teaching Hospital from 2001 to 2005. There were 10 adult female buffalos with olecranon bursitis (one had bilateral bursitis) and 26 calves (7 cattle and 19 buffalos, 16 males and 10 females) with presternal bursitis. There were 10 out of 11 cases of olecranon bursitis and 21 out of 26 cases of presternal bursitis with different forms (cystic, proliferative and fibrous) that were removed surgically. The remaining 6 cases, cystic bursitis (olecranon = 1, presternal = 5), were treated by aspiration of their contents and injection of 4% iodine tincture intrabursally. Only 2 cases recovered, 3 cases progressed to fibrosis and required further surgical treatment 2 to 3 weeks later, and 1 case continued to have a cystic lesion. Histopathological examination of tissue specimens from different forms of bursitis revealed that the acquired bursae were generally lined with synovial-like membrane formed from 2-3 cellular layers that covered the connective tissue capsule. The connective tissue capsule differed from one type to another and consisted of fibrous tissues containing numerous small blood vessels, blood capillaries, lymphatics and nerves. There was also evidence for inflammation within the capsule represented by congestion of blood vessels and the presence of perivascular inflammatory cells, mostly mononuclear. In conclusion, surgical treatment was successful and effective for treatment of olecranon and presternal bursitis particularly for the chronic proliferative and fibrous form in cattle and buffalo. The histological structure of the acquired bursae was relatively similar consisting of a synovial-like membrane and a connective tissue capsule with varying degrees of the inflammatory process.  相似文献   
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Tropical Animal Health and Production - The present study was conducted to determine the prevalence of fasciolosis and investigate the associated risk factors (sex, age, and season) in slaughtered...  相似文献   
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Tropical Animal Health and Production - There is scarce data available on helminth infection of poultry in the region of Aswan province, Upper Egypt. A total of 276 poultry (156 baladi chickens and...  相似文献   
69.
The objectives were to evaluate the reproductive indices and survival analysis of pregnancy outcomes in multiparous repeat breeder Holstein cows (n = 557). The cows were synchronized to ovulate by Ovsynch, new controlled internal drug release device (N‐CIDRsynch), and once‐used CIDR device (U‐CIDRsynch). The pregnancy per AI at 28 days post‐insemination (P/AI 28) in the N‐CIDRsynch group (28.75%) was significantly (COR = 1.49; p = 0.011) greater than that reported in the Ovsynch (23.46%) and U‐CIDRsynch (21.73%) groups. Furthermore, the pregnancy per AI at day 75 post‐insemination (P/AI 75) in the N‐CIDRsynch group was significantly greater than the Ovysync group (COR = 1.35; p = 0.050). The repeat breeder cows received a N‐CIDR device had a significantly higher progesterone level on day 2 and day 4 of CIDR insertion (1.38 and 1.67 ng/ml, respectively) than those received a U‐CIDR device or the control group (p = 0.012 and 0.001, respectively). The Cox regression model recorded significant associations for synchronization protocols, THI at the TAI and season of calving with the hazard of P/AI 28 and P/AI 75 (p = 0.044 and 0.046; 0.001 and 0.005; 0.003 and 0.001, respectively). Multiparous repeat breeder cows (>3) had a lower hazard ratio (HR) of P/AI 28 than that reported in the reference (2nd parity) group (HR = 0.74, p = 0.050). The repeat breeder cows inseminated at 76–80 and >80 temperature‐humidity index (THI) had significantly lower HR of P/AI 28 than those inseminated at the baseline (<70) THI value (HR = 0.73 and 0.30, p = 0.036 and 0.001, respectively). The current results indicate that the use of N‐CIDR synch protocol may achieve satisfactory pregnancy outcomes in repeat breeder cows.  相似文献   
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