Differentiation of soil fauna in desert agriculture of the Mariut region

Summary The soil fauna of 23 sites in the Mariut region, west of Alexandria, Egypt, was sampled monthly or seasonally for periods of 1,5–2.5 years. These sites range from unutilized littoral sand dunes, to free-grazing pasture, to low- and high-intensity dry farming, to irrigated agriculture. The average population densities of the encountered taxa were compared by corresponding analysis and by ascending hierarchic classification. With intensification of the agricultural practices, true detritivores of the original system, capable of dealing with the available plant and animal litter and adapted to the harsh desert climate, are replaced by other mesic detritivores adapted to the changes in litter quality (less animal and more plant) and also adapted to the new physical and chemical soil conditions. Meanwhile, the increase in plant biomass encourages the appearance and proliferation of cryptic phytophages (agricultural pests), occupying the niche of the removed large (domestic) herbivores. Carnivores (e.g.Carabidae) also change accordingly. Sites can thus be characterized on the basis of their soil faunas, according to the stage of their evolution, and a system of exchange of missing key taxa between sites should be established.     

Diversity of native rice (Oryza Poaceae:) species of Costa Rica

We found several populations of wild Oryza species in the lowlands of Costa Rica. The plants showed extensive morphological variation, suggesting the presence of several species. In the morphologic study, 33 traits were scored for plants of all the species. A principal component analysis revealed the significant morphological separation of the different species. The analyses indicated that there are three species, O. grandi­glumis, O. latifolia and O. glumaepatula. Two putative hybrid types were found, both significantly differing in their morphology from the known species and intermediate at several traits. O. grandi­glumis is a new record for Costa Rican flora. Its main population is located in Caño Negro Wildlife Refuge, Los Chiles, Alajuela. O. latifolia is distributed throughout the lowlands of the country and the plants of the Atlantic slope are significantly bigger in general habit than those of the Guanacaste area. During this study a population of O. glumaepatula of hundreds of thousands of plants was discovered in the Medio Queso River wetland, Los Chiles, Alajuela. This population is the most important source of genes for cultivar's improvement from the primary gene pool of rice in Costa Rica. The small ligule and the wide flag leaf characteristic of the two CCDD species separated them from the AA diploid O. glumaepatula. Seed size, ligule size, number of branches in the panicle, plant height and sterile lemma length are all bigger in O. grandi­glumis, and influenced the second factor that separated the CCDD species in two discrete clusters. The species found offer great possibilities for the improvement of rice cultivars and they should be thoroughly studied and appropriately protected.     

8S globulin of mungbean [Vigna radiata (L.) Wilczek]: cloning and characterization of its cDNA isoforms, expression in Escherichia coli, purification, and crystallization of the major recombinant 8S isoform

Three isoforms of the cDNA of the major 8S globulin of mungbean, 8Salpha, 8Salpha', and 8Sbeta, were isolated, cloned, and characterized. The cDNA sequences of 8Salpha, 8Salpha', and 8Sbeta had open reading frames of 1362, 1359 or 1362, and 1359 bp, respectively, which code for 454, 453 or 454, and 453 amino acids corresponding to molecular weights of 51 973, 51 627 or 51 758, and 51 779, respectively. Homology in terms of cDNA and amino acid sequences was 91-92% between 8Salpha and 8Salpha', 87% between 8Salpha and 8Sbeta, and 86-88% between 8Salpha' and 8Sbeta. The signal peptide was found to be 1-25, 1-24 or 25, and 1-23 for 8Salpha, 8Salpha', and 8Sbeta, respectively, using the signalP website (Nielsen, H.; Engelbrecht, J.; Brunak, S.; von Heijne, G. Protein Eng. 1997, 10, 1-6). The propeptide was determined to be IVHREN. A single site for glycosylation (N-X-S/T) was observed about 90 amino acids from the C terminus. Homology between mungbean 8S isoforms and other 7-8S proteins ranged from 45 to 68% within members of the legume family and 29 to 34% for crops of different species. The major isoform 8Salpha was expressed in Escherichia coli and purified by successive ammonium sulfate fractionation, hydrophobic interaction, and Mono Q column chromatography. The recombinant 8Salpha, but not the native form, was successfully crystallized producing rhombohedral crystals.     

Conservation value of dispersed tree cover threatened by pasture management

Trees dispersed in pastures are a prominent feature of many Central American landscapes, particularly in cattle producing regions where farmers retain trees to serve as shade, fodder, timber and firewood. The presence of dispersed trees in pastures is often considered as important for the conservation of biodiversity by providing habitat and enhancing landscape connectivity. However, despite their critical productive and environmental roles, little is known about tree distribution within pastures or how farmers’ management decisions influence the trees themselves and their impact on farm productivity and biodiversity conservation. Here, we present a synthesis of (a) the abundance, composition, and size of dispersed trees in four important cattle producing regions of Costa Rica (Caňas and Río Frío) and Nicaragua (Rivas and Matiguás), based on inventory of 18,669 trees on 1492 ha of pasture, (b) the local knowledge, management and use of trees by cattle farmers, and (c) opportunities for ensuring sustainable management of dispersed trees in pasture-dominated landscapes. Dispersed trees were common in all four landscapes, with mean frequency ranging from 8.0 trees ha⁻¹ in Caňas to 33.4 trees ha⁻¹ in Matiguás. A total of 255 tree species were found in pastures across the four landscapes. The total number of tree species per landscape varied from 72 in Rivas to 101 in Caňas and Rio Frio, with mean species richness per farm ranging from 22.9 in Rio Frio to 45.9 in Matiguás. In all four landscapes, a handful of tree species dominated the pastures, with the ten most abundant species in each landscape accounting for >70% of all trees recorded. Most of these common tree species provide fruits or foliage eaten by cattle, or are important timber or firewood species, and are deliberately retained by farmers for these uses. In all four landscapes, farmers had a detailed knowledge of tree attributes affecting pasture and animal productivity, and influenced tree cover through pasture management activities and occasional tree cutting. Current farm management practices are gradually decreasing the diversity of trees in pastures, and in some cases also tree density, reducing their contribution to farm productivity and biodiversity conservation. To reverse this trend, incentives are required to encourage cattle farmers to retain and enhance tree cover in pastures, through the adoption of pasture management practices that favor the regeneration and persistence of a diverse range of tree species.