笼养鸡舍鸡粪固态处理系统的研究

上海出现多种笼养鸡舍液态鸡粪的处理方法，似均存在投资大、运行费用高、操作管理复杂、技术不过关或不配套等缺点。作者设计了“鸡粪固态处理系统”，其思路是在笼养鸡舍内就使粪与饮水严格分流。本文介绍了该系统的工艺流程、设施改造。日常管理等。实践证明该系统具有投资很少、运行费用甚低。操作管理十分简单、不占用土地和效益相当突出的优点，确是一项简易的实用技术。     

摆动平底从动件盘形凸轮廓解析设计的应用

本文根据具体机器动作协调性要求及其具体空间几何条件,采用了摆动从动件盘形凸轮机构。其摆杆推程在O°～52°及52°～62°范围内分别采用了等加速——等速合理拼接运动规律。回程在62°～153°范围内采用了正弦加速度运动规律。用极坐标矢量分析法建立方程、微分,通过电脑程序计算,设计制造出凸轮轮廓,用于禾苗移植机的擒纵机构处,完全符合生产要求,获得了理想的效果。     

光箨篌竹笋期生长规律初步研究

本文对光箨篌竹笋期的出笋规律、成竹规律以及竹笋的生长发育规律进行了研究。初步认为,光箨篌竹林分的母竹立竹度一般应保持在2500株/亩左右、Ⅰ～Ⅱ级母竹株数保持在40％以上时,才能保证林分的优质高产与稳产;抽枝成竹历期长短受竹笋笋级大小、出土早晚或立地环境条件的影响可相应延长或推迟2～4d左右,一般历时25d,竹笋开始抽枝成竹时的高度一般为5m左右;竹笋在生长发育高峰期明显受到气候因子的影响,尤其是对气温更为敏感。     

仙客来离体叶培养与植株再生

以仙客来叶片、叶柄及其不同部位为外植体进行诱导,结果幼叶基部(叶子基部与叶柄相连处的断口部分)诱导愈伤组织频率最高,可达100％。愈伤组织在N_6+BA2mg/1+2,4-D0.5mg/1培养基上培养,约40d左右可100％分化出不定芽,其不定芽数量一块愈伤组织最多可分化出30个以上。将幼苗移入1/2MS+NAA 0.5mg/1培养基中生根最好。     

环境温度和饲粮营养浓度对生长期母兔代谢能利用率的影响

选用6只9周龄新西兰母兔,评定环境温度和饲粮营养浓度对生长期母兔代谢能利用率的影响。试验在密闭式的小动物呼吸测热柜中进行。分别对处于环境温度为20±2℃和30±2℃,采食两种不同营养浓度的试兔进行测试。饲粮营养水平实测值,分别为12.22kJ ME/g和13.49kJ ME/g;粗蛋白质(CP)分别为17.3％和21.6％;中性洗涤纤维(NDF)分别为23.7％和16.5％。结果表明:环境温度影响试兔的能量平衡,它与采食量间存在着显著的相关。20±2℃时,相关系数r=0.798;30±2℃时,相关系数r=0.875。在本试验设计范围内,饲粮营养浓度并不影响代谢能食入量(ME_I),热增耗(HP)和能量平衡(EB)。当环境温度为20±2℃和30±2℃时,表观代谢能转化率(NE/ME)分别为51％和71％;每天每兔每千克代谢体重代谢能维持需要分别为396 kJ和361 kJ。试验结果认为:环境温度影响营养物质消化率(P相似文献   

不同氮源对红檬实生苗菌根依赖性的影响

将红檬实生苗培养于改良无土基质中，接种泡囊－丛枝菌根Ｇｌｏｍｕｓｃｉｔｒｏｃｏｌｕｍ，以不接种菌根为对照，并分别施用含氮量相等的３种氮肥，即硝酸按（ＮＯ３－Ｎ）、尿素（ＮＨ２－Ｎ）和硫酸铵（ＮＨ４－Ｎ）。接种菌根１６周后，施不同氮肥的红檬实生苗均有效地感染了菌根，并促进了植株的生长。试验证明，红实生苗施硝态氮（ＮＯ３－Ｎ）使植株的菌根依赖性有明显提高。     

单克隆抗体ELISA检测犊牛粪便中冠状病毒抗原

用两株单克隆抗体混合后包被ＥＬＬＩＳＡ微孔板，加牛冠状病毒抗原，加经白陶土处理过的兔抗牛冠状病毒免血清，再加上辣根氧化物酶标记的羊抗兔ＩｇＧ抗体，加底物质显色间接ＥＬＩＳＡ检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液，其敏感度高出血凝试验（ＨＡ）８倍，从武汉市附近３个奶牛场采集犊牛腹泻粪便１０５份，用单抗ＥＬＩＳＡ检测牛冠状病毒抗原，共检出阳性３６份，并与血凝及血凝抑制试验，     

Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.

In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals.     

Inheritance of myotonic discharges in American quarter horses and the relationship to hyperkalemic periodic paralysis.

Electromyography (EMG) was used to detect myotonic discharges in Quarter Horse breeding stock and to follow the results of mating horses with hyperkalemic periodic paralysis (HPP). The studies were performed on two brood mare farms. A total of six breeding stock showed myotonic discharges and 15 were nonmyotonic. Myotonic discharges were seen in five of six horses belonging to the blood line previously implicated as being predisposed to HPP. Two of these horses had shown clinical signs of HPP. Only one of 15 breeding horses unrelated to the HPP predisposed blood line showed myotonic discharges. When both parents were non-myotonic on EMG than the F1 generation (n = 6) were also nonmyotonic. When a stallion with HPP and myotonic discharges was mated to eight nonmyotonic mares over a six year period half the animals of the F1 generation (n = 25) showed myotonic discharges. When both parents showed myotonic discharges four F1 offspring were myotonic and two were nonmyotonic on EMG testing. There was no evidence of sex linkage. The results are consistent with an autosomal dominant mode of inheritance. Hyperkalemic periodic paralysis and myotonic discharges on EMG may be different manifestations of the same underlying defect.