Excavatoids O and P, new 12-hydroxybriaranes from the octocoral Briareum excavatum

Two new 12-hydroxybriarane diterpenoids, designated as excavatoids O (1) and P (2), were isolated from the octocoral Briareum excavatum. The structures of briaranes 1 and 2 were established on the basis of extensive spectral data analysis. Excavatoid P (2) is the first metabolite which possesses a 6β -chlorine atom in briarane analogues.     

Cloning and tissue expression of the equine transferrin receptor

Background: Characterization of anemia in horses presents a challenge, as they do not release reticulocytes into peripheral blood. Transferrin receptor (TfR) expression is highest on erythroid cells in people and rats, and measurement of a soluble serum form (sTfR) is used to quantify erythropoiesis in these species. We hypothesized that equine TfR (eTfR) expression is similar in quantity and distribution to that in these other species and thus has potential for characterization of the regenerative response in anemic horses. Objective: This study was conducted to clone and sequence the eTfR gene and measure expression levels using quantitative real‐time PCR and immunohistochemical (IHC) staining. Methods: Total RNA from equine bone marrow was used to produce cDNA. The eTfR gene was amplified using pooled gene‐specific primers, and PCR products were sequenced. Rapid amplification of cDNA ends was used to obtain the first 22 nucleotides of the coding sequence. Quantitative PCR was performed using eTfR gene‐specific primers, and IHC staining was used to localize TfR protein expression. Results: The deduced amino acid (aa) sequence (767 aa) of the eTfR was 75–83% identical with sequences of the receptor in several other mammals. As in people and rats, eTfR mRNA expression was highest in the bone marrow, and distribution in other tissues was also similar. Conclusion: The eTfR gene is similar to that of other mammals in structure and expression levels. We hypothesize that it is also similar in function and that, following development of an immunoassay, determining sTfR concentrations will be useful for identifying the regenerative response in anemic horses.     

Growth of Black Currant Infested with Currant-Lettuce Aphid

Young, pot-grown black currant bushes, var. Wellington XXX, were artificially infested with the currant-lettuce aphid (Hyperomyzus lactucae L.) and their growth until mid-July was compared with unsprayed, aphid-free, controls.     

Treatment of a malignant pheochromocytoma in a dog using 131I metaiodobenzylguanidine

A 12 yr old castrated male Yorkshire terrier was presented with a history of an inoperable pheochromocytoma. Physical examination revealed a large, midabdominal mass. Neurologic examination was normal at presentation. An abdominal computed tomography scan revealed a 215 cm(3) mass in the region of the right kidney. Forty-eight hours after IV injection of 370 megabecquerels (MBq, equivalent to10 millicuries [mCi]) of metaiodobenzylguanidine labeled with radioactive iodine ([(131)I]MIBG), standard planar scintigraphy was performed. A diffuse area of moderate uptake was noted in the midabdominal region. The dog experienced stable disease for 1.5 mo after injection based on a follow-up computed tomography (CT) scan; however, 5 mo after injection, repeat CT imaging revealed progression of the tumor, and a second IV injection of 370 MBq (10 mCi) of [(131)I]MIBG was administered. The dog died 3 wk after the second injection as a result of gastrointestinal blood loss that was believed to be caused by compression-induced bowel ischemia by the mass. A full necropsy was not performed, but the mass was removed for histologic evaluation, which confirmed the diagnosis of pheochromocytoma. This report is the first to document the treatment of canine pheochromocytoma using [(131)I]MIBG.     

Does pollarding trees improve the crop yield in a mature alley-cropping agroforestry system?

In agroforestry systems, the canopy of maturing trees progressively reduces the radiation available for the crop. Tree management practices such as pollarding can be used to restore the crop light availability. We monitored durum wheat, barley and pea yields during three consecutive years in an alley-cropping agroforestry system with hybrid walnut trees in Southern France. Trees have been pruned regularly to 4 m since planting (in 1995), and a section of 50 trees was pollarded at 4 m height for the first time in 2013. We measured the yields and yields components in the two different tree management systems and in a sole crop control. The non-pollarded trees reduced significantly the incident light (around 80% of the global radiation left in average) and both wheat (68% of the control) and pea (69% of the control), but not barley mean yields. In pollards inter-row, the average incident light was 98% of the total radiation the first year and the wheat yield 89% of the control. But 3 years after pollarding, the incident light was 79% of the total radiation and the pea yield was only 67% of the control. Pollarding had a transient positive impact on crop yield; after 3 years, pollards were more competitive for light than control trees. Pollarding may also reduce the belowground trees competition, but slightly, as the crop vegetative growth occurs when the trees are leafless.