全文获取类型
收费全文 | 100011篇 |
免费 | 5586篇 |
国内免费 | 91篇 |
专业分类
林业 | 4374篇 |
农学 | 3388篇 |
基础科学 | 657篇 |
12931篇 | |
综合类 | 15394篇 |
农作物 | 3931篇 |
水产渔业 | 5121篇 |
畜牧兽医 | 51868篇 |
园艺 | 1181篇 |
植物保护 | 6843篇 |
出版年
2019年 | 1032篇 |
2018年 | 1526篇 |
2017年 | 1685篇 |
2016年 | 1617篇 |
2015年 | 1301篇 |
2014年 | 1632篇 |
2013年 | 3910篇 |
2012年 | 2963篇 |
2011年 | 3432篇 |
2010年 | 2268篇 |
2009年 | 2313篇 |
2008年 | 3415篇 |
2007年 | 3276篇 |
2006年 | 2965篇 |
2005年 | 2836篇 |
2004年 | 2620篇 |
2003年 | 2780篇 |
2002年 | 2558篇 |
2001年 | 3065篇 |
2000年 | 3001篇 |
1999年 | 2412篇 |
1998年 | 985篇 |
1997年 | 1048篇 |
1995年 | 1106篇 |
1994年 | 948篇 |
1993年 | 969篇 |
1992年 | 1890篇 |
1991年 | 2100篇 |
1990年 | 2005篇 |
1989年 | 1993篇 |
1988年 | 1861篇 |
1987年 | 1808篇 |
1986年 | 1846篇 |
1985年 | 1802篇 |
1984年 | 1564篇 |
1983年 | 1328篇 |
1982年 | 967篇 |
1979年 | 1502篇 |
1978年 | 1148篇 |
1977年 | 1076篇 |
1976年 | 1045篇 |
1975年 | 1101篇 |
1974年 | 1332篇 |
1973年 | 1343篇 |
1972年 | 1266篇 |
1971年 | 1182篇 |
1970年 | 1143篇 |
1969年 | 1141篇 |
1968年 | 991篇 |
1967年 | 1122篇 |
排序方式: 共有10000条查询结果,搜索用时 19 毫秒
51.
52.
53.
54.
55.
Herrick GW 《Science (New York, N.Y.)》1928,68(1759):237-244
56.
57.
58.
59.
Antiserum to a partially purified neuraminidase fromPasteurella multocida, type A:3, was adsorbed with protease-digestedP. multocida type 3 lipopolysaccharide (LPS) to remove LPS immunoreactivity. The LPS-adsorbed antineuraminidase caused a 77% reduction in the neuraminidase activity of homologousP. multocida in anin vitro enzyme neutralization test. All 14 mice passively immunized with the adsorbed antineuraminidase were protected against challenge infection with homologousP. multocida in a mouse protection test. Ten out of 14 mice in one group that received antisera containing antibodies to both neuraminidase and LPS were protected. In contrast, only 1 out of 14 mice that were immunized with pre-immune serum survived the challenge. These results suggest that antiserum toP. multocida neuraminidase was, at least partly, responsible for the protection observed in this study. Neuraminidase may be one of the immunogenic protective proteins present in aqueous extracts ofPasteurella multocida. 相似文献
60.
T Sekizaki Y Nakasato I Nonomura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(3):493-499
Chicken pathogenic Escherichia coli strains were found to autoagglutinate in a static culture of trypticase soy broth (TSB). One strain, designated PDI-386, was further studied for its autoagglutinating property. Acidity in the cultured medium caused by glucose degradation induced the autoagglutination. The bacterial cells grown in a glucose-free L-broth could be aggregated by adding acid, which suggests a potentiality of autoagglutination of the strain grown in the L-broth. The autoagglutinating parent (Agg) formed small colonies with irregular edges like rough colonies on the TS agar, whereas its non-autoagglutinating variant (Nag) formed larger smooth colonies with a perfectly round edge. The Nag colony was easily generated from the Agg colony on the TS agar. The autoagglutinating property was very unstable when the bacteria was passed in the TSB, but rather stable in the L-broth. Under electron microscope, the Agg were found to possess pili of more than 20 microns in length. However, the phenotypic expression of autoagglutination did not correlate with that of mannose-sensitive hemagglutination against guinea pig erythrocytes. Incubation of the Nag in the L-broth at room temperature for more than 10 days provoked the reversion of the autoagglutination. There was no difference between the Agg and the Nag in terms of surface hydrophobicity, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of membrane proteins and LPS, and plasmid profiles. The virulence of the Agg was higher than that of the Nag. The autoagglutination property is, however, so unstable that the pathogenicity of E. coli isolates from chickens should be carefully evaluated. 相似文献